Construction and Antibacterial Activity Evaluation of a Lipopeptide Delivery System

Authors

• Rong CHEN, Sichuan Baoning Vinegar Co ., Ltd., Langzhong 637400 , ChinaFollow
• Mingye GUO, Sichuan Baoning Vinegar Co ., Ltd., Langzhong 637400 , China
• Ge HOU, Sichuan Baoning Vinegar Co ., Ltd., Langzhong 637400 , China
• Yan CHEN, Sichuan Baoning Vinegar Co ., Ltd., Langzhong 637400 , China

Corresponding Author(s)

陈蓉（1982—），女，学士，主要从事调味品、肉制品研究。E-mail：18188530@qq.com

Abstract

[Objective ] This study constructed a stable lipopeptide (LP) delivery system to improve the stability and dispersibility of LP,thereby enhancing their antibacterial efficacy.[Method ] LP particles were constructed with chitosan (CS) and sodium alginate (SA).Assembly property changes were evaluated by means of different construction and single factor experiments.Interactions among CS,LP,and SA were investigated by Fourier transform-infrared spectroscopy (FT-IR ),and the rheological properties of the system were examined by a rheometer.Antibacterial activity of LP particles was evaluated with Escherichia coli (E.coli) and Staphylococcus aureus (S.aureus ) as model microorganisms.[Result ] LP particles prepared by dripping CS with the relative molecular mass of 300 000 into a mixed solution of SA (low viscosity ) and LP at the CS∶LP∶SA mass ratio of 1∶1∶1,150 r/min,and pH 4 achieved the best encapsulation performance.The LP particles prepared under the conditions above showed the encapsulation rate of (92.95±1.31)%,loading content of (41.14±1.63)%,particle size of 420~489 nm,and Zeta potential of (–21.56±3.54) mV.FT-IR results indicated that LP was encapsulated inside the particles,with SA forming the outer shell.Rheological data confirmed a hydrogel-like structure.In addition,LP particles showed strong inhibitory effects against both E.coli and S.aureus and resistance to thermal and UV stress.[Conclusion ] LP particles constructed in this study not only reduce LP aggregation but also provides effective protection against environmental stress,offering a promising strategy for the delivery of LP-based antimicrobials in fields such as food preservation.

Publication Date

1-15-2026

First Page

154

Last Page

164

DOI

10.12441/spyswjs.20251028001

Recommended Citation

CHEN, Rong; GUO, Mingye; HOU, Ge; and CHEN, Yan (2026) "Construction and Antibacterial Activity Evaluation of a Lipopeptide Delivery System," Journal of Food Science and Biotechnology: Vol. 45: Iss. 1, Article 15.
DOI: 10.12441/spyswjs.20251028001
Available at: https://spsw.spyswjs.cnjournals.com/journal/vol45/iss1/15

References

［1］ 翟世玉.枯草芽孢杆菌 LF17鉴定及对苹果树腐烂病生防作用效果研究 ［D］.太谷：山西农业大学，2019.
［2］ 李紫英，沙帅帅，唐御忻，等.贝莱斯芽孢杆菌 SY01诱导苹果树抗性基因表达及其脂肽类抑菌物质分析 ［J］.山东农业大学学报 （自然科学版 ），2024，55（5）：711-719.LI Z Y，SHA S S，TANG Y X，et al.Expression of resistance genes induced by Bacillus velezensis SY 01 in apple trees and analysis of its lipopeptide inhibitory substances ［J］.Journal of Shandong Agricultural University （Natural Science Edition ），2024，55（5）：711-719.（in Chinese ）
［3］ YANG L X，CHEN C X，LIANG T T，et al.Disassembling ability of lipopeptide promotes the antibacterial activity ［J］.Journal of Colloid and Interface Science，2023，649：535-546.
［4］ SABERI RISEH R，VATANKHAH M，HASSANISAADI M，et al.A review of chitosan nanoparticles：nature’s gift for transforming agriculture through smart and effective delivery mechanisms ［J］.International Journal of Biological Macromolecules，2024，260：129522.
［5］ BERNKOP-SCHNÜRCH A，DÜNNHAUPT S.Chitosan-based drug delivery systems ［J］.European Journal of Pharmaceutics and Biopharmaceutics，2012，81（3）：463-469.
［6］ XU Y S，LIU M M，FAISAL M，et al.Selective protein complexation and coacervation by polyelectrolytes ［J］.Advances in Colloid and Interface Science，2017，239：158-167.
［7］ CHEN B Y，AI C，HE Y J，et al.Preparation and structural characterization of chitosan-sodium alginate nanocapsules and their effects on the stability and antioxidant activity of blueberry anthocyanins ［J］.Food Chemistry，2024，23：101744.
［8］ NIKOLOVA D，SIMEONOV M，TZACHEV C，et al.Polyelectrolyte complexes of chitosan and sodium alginate as a drug delivery system for diclofenac sodium ［J］.Polymer International，2022，71（6）：668-678.
［9］ 井勇慧，李桥，康丽，等.响应面法优化山楂多酚微粒制备工艺［J］.食品工业科技，2024，45（20）：187-195.JING Y H，LI Q，KANG L，et al.Optimization of preparation process for hawthorn polyphenol microparticles based on response surface methodology ［J］.Science and Technology of Food Industry，2024，45（20）：187-195.（in Chinese）
［10］ SAE THER H V，HOLME H K，MAURSTAD G，et al.Polyelectrolyte complex formation using alginate and chitosan［J］.Carbohydrate Polymers，2008，74（4）：813-821.
［11］ 李光月，李雪玲，祁姣姣，等.响应面法优化枯草芽孢杆菌表面活性素的发酵工艺 ［J］.食品工业科技，2022，43（12）：146-154.LI G Y，LI X L，QI J J，et al.Optimization of fermentation conditions of surfactin from Bacillus subtilis by response surface methodology ［J］.Science and Technology of Food Industry，2022，43（12）：146-154.（in Chinese）
［12］ 戴照琪，赵见营，张一凡，等.壳聚糖缓释抑菌纳米涂膜材料制备及对采后草莓品质的影响 ［J］.食品科技，2024，49（11）：37-45，55.DAI Z Q，ZHAO J Y，ZHANG Y F，et al.Preparation of chitosan sustained release antibacterial nanofilm material and its effect on postharvest strawberry quality ［J］.Food Science and Technology，2024，49（11）：37-45，55.（in Chinese）
［13］ 施佳利.海藻酸钠基水凝胶药物载体的制备及性能研究［D］.淮南：安徽理工大学，2024.
［14］ STETEFELD J，MCKENNA S A，PATEL T R.Dynamic light scattering：a practical guide and applications in biomedical sciences ［J］.Biophysical Reviews，2016，8（4）：409-427.
［15］ LIU W，LIU Y M，ZHU R，et al.Structure characterization，chemical and enzymatic degradation，and chain conformation of an acidic polysaccharide from Lycium barbarum L.［J］.Carbohydrate Polymers，2016，147：114-124.
［16］ SU C Y，LI D，WANG L J，et al.Green double crosslinked starch-alginate hydrogel regulated by sustained calcium ion-gluconolactone release for human motion monitoring ［J］.Chemical Engineering Journal，2023，455：140653.
［17］ GIUNTOLI A，PUOSI F，LEPORINI D，et al.Predictive relation for the α relaxation time of a coarse-grained polymer melt under steady shear ［J］.Science Advances，2020，6（17）：eaaz 0777.
［18］ 纪帅奇，乌日娜，张淘崴，等.芽孢杆菌脂肽对金黄色葡萄球菌的抑制机理 ［J］.中国食品学报，2024，24（8）：197-205.JI S Q，WU R N，ZHANG T W，et al.The antimicrobial mechanism of Bacillus lipopeptide against Staphylococcus aureus［J］.Journal of Chinese Institute of Food Science and Technology，2024，24（8）：197-205.（in Chinese ）
［19］ MIHAI M，GHIORGHITA C A，STOICA I，et al.New polyelectrolyte complex particles as colloidal dispersions based on weak synthetic and/or natural polyelectrolytes［J］.Express Polymer Letters，2011，5（6）：506-515.
［20］ HU X Y，WANG Y M，ZHANG L L，et al.Formation of self-assembled polyelectrolyte complex hydrogel derived from salecan and chitosan for sustained release of vitamin C ［J］.Carbohydrate Polymers，2020，234：115920.
［21］ TAN C，XIE J H，ZHANG X M，et al.Polysaccharide-based nanoparticles by chitosan and gum Arabic polyelectrolyte complexation as carriers for curcumin ［J］.Food Hydrocolloids，2016，57：236-245.
［22］ SIMSEK-EGE F A，BOND G M，STRINGER J.Polyelectrolyte complex formation between alginate and chitosan as a function of pH ［J］.Journal of Applied Polymer Science，2003，88（2）：346-351.
［23］ POTAŚ J，SZYMAŃSKA E，WINNICKA K.Challenges in developing of chitosan：based polyelectrolyte complexes as a platform for mucosal and skin drug delivery［J］.European Polymer Journal，2020，140：110020.
［24］ KANG L，LI Q，JING Y H，et al.Auricularia auricula anionic polysaccharide nanoparticles for gastrointestinal delivery of Pinus koraiensis polyphenol used in bone protection under weightlessness ［J］.Molecules，2024，29（1）：245.
［25］ JIN M L，WANG Y M，XU C L，et al.Preparation and biological activities of an exopolysaccharide produced by Enterobacter cloacae Z0206［J］.Carbohydrate Polymers，2010，81（3）：607-611.
［26］ GERICKE M，GABRIEL L，GEITEL K，et al.Synthesis of xylan carbonates：an approach towards reactive polysaccharide derivatives showing self-assembling into nanoparticles ［J］.Carbohydrate Polymers，2018，193：45-53.
［27］ TAN S L，LEE H Y，MAHYUDIN N A.Antimicrobial resistance of Escherichia coli and Staphylococcus aureus isolated from food handler ’s hands［J］.Food Control，2014，44：203-207.
［28］ MEKA V S，SING M K G，PICHIKA M R，et al.A comprehensive review on polyelectrolyte complexes ［J］.Drug Discovery Today，2017，22（11）：1697 -1706.