Ultrasound-assisted Dual-enzyme Extraction Condition Optimization and Antioxidant Activity Evaluation of Anthocyanins from Perilla Leaves

Authors

• Wenting ZHU, School of Environmental and Biological Engineering , Wuhan Technology and Business University , Wuhan 430065 , ChinaFollow
• Shiyun WANG, School of Environmental and Biological Engineering , Wuhan Technology and Business University , Wuhan 430065 , China
• Qiaolin ZHU, School of Environmental and Biological Engineering , Wuhan Technology and Business University , Wuhan 430065 , China
• Qi CAO, School of Environmental and Biological Engineering , Wuhan Technology and Business University , Wuhan 430065 , China
• Yixin ZHANG, School of Environmental and Biological Engineering , Wuhan Technology and Business University , Wuhan 430065 , China
• Tianran HONG, School of Environmental and Biological Engineering , Wuhan Technology and Business University , Wuhan 430065 , China

Corresponding Author(s)

朱文婷（1983—），女，硕士，副教授，主要从事生物化学与分子生物学研究。E-mail：251558801@qq.com

Abstract

[Objective ] This study aims to comprehensively develop perilla and improve its utilization value.[Method ] Using perilla leaves as raw material,the effects of solid-liquid ratio,ethanol volume fraction,ultrasonic time,and enzymatic hydrolysis time on the mass fraction of anthocyanins in perilla leaves were studied.The ultrasound-assisted dual-enzyme extraction conditions for anthocyanins from perilla leaves were optimized by the response surface method.The antioxidant activity and phenol mass fraction of anthocyanins from perilla leaves were determined,and the main phenols were analyzed by chromatography.[Result ] The optimal extraction conditions of anthocyanins from perilla leaves were solid-liquid ratio of 1 g∶16 mL,ethanol volume fraction of 74%,ultrasonic time of 23 min,and enzymatic hydrolysis time of 92 min.Under these conditions,the mass fraction of anthocyanins from perilla leaf was 252.87 mg/ 100 g,with a DPPH radical scavenging rate of 48.88%,a total antioxidant capacity of 51.12%,and a hydroxyl radical scavenging rate of 34.99%.The phenols in perilla leaf anthocyanins showed the mass fraction of 4.81 mg/g,containing (0.43±0.04) mg/g delphinidin- 3-O-glucoside,(0.86±0.07) mg/g cyanidin,and (1.41±0.03) mg/g paeoniflorin- 3,5-O-diglucoside.The mass fraction of phenols in different batches of the same variety had little difference.In addition,perilla leaf anthocyanins had a strong ability to scavenge DPPH and hydroxyl free radicals.Paeoniflorin- 3,5-O-diglucoside bound to the Nrf 2 pocket of KEAP 1,inhibited KEAP 1-Nrf2 binding,and promoted the nuclear translocation of Nrf 2 and the expression of hemeoxygenase- 1 (HO- 1) and quinone oxidoreductase 1 (NQO 1).[Conclusion ] Factors such as the mass ratio of dual enzymes,total enzyme dosage,temperature,solid-liquid ratio,ethanol volume fraction,ultrasonic time,and enzymatic hydrolysis time have different effects on the mass fraction of anthocyanins in perilla leaves,and the mass fraction of phenols in perilla leaves is related to the raw materials and storage conditions.Perilla leaf anthocyanins can exert antioxidant effects by directly scavenging free radicals and activating the KEAP 1-Nrf2/antioxidant response element (ARE ) pathway.Ultrasound-assisted dual-enzyme method enables efficient extraction of high-purity anthocyanins from perilla leaves.This study provides a theoretical basis for the application of perilla leaf anthocyanins in food processing.

Publication Date

1-15-2026

First Page

130

Last Page

140

DOI

10.12441/spyswjs.20241213001

Recommended Citation

ZHU, Wenting; WANG, Shiyun; ZHU, Qiaolin; CAO, Qi; ZHANG, Yixin; and HONG, Tianran (2026) "Ultrasound-assisted Dual-enzyme Extraction Condition Optimization and Antioxidant Activity Evaluation of Anthocyanins from Perilla Leaves," Journal of Food Science and Biotechnology: Vol. 45: Iss. 1, Article 13.
DOI: 10.12441/spyswjs.20241213001
Available at: https://spsw.spyswjs.cnjournals.com/journal/vol45/iss1/13

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