Journal of Food Science and Biotechnology
Abstract
[Objective ] This study aims to establish the biosynthesis process of N-acetyl- trans -4-hydroxyproline (N-ATHOP ).[Method ] Escherichia coli (E.coli ) BL21 (DE3) was selected as the host to heterologously express the acyltransferase (MsAcT ) derived from Mycobacterium smegmatis.A semi-rational design was employed to construct the mutant MsAcT (S11C) capable of catalyzing the synthesis of N-ATHOP.[Result ] The catalytic reaction conditions of the mutant MsAcT (S11C) were optimized as pH 6.9,reaction temperature 49 ℃,initial trans -4-hydroxyproline (THOP ) concentration of 315.5 mmol/L,and vinyl acetate volume fraction of 10%.Under the optimized conditions,the yield of N-ATHOP synthesized under the catalysis of MsAcT (S11C) reached 4.71 g/L,with a conversion rate of 10.95% after 12 h of reaction.[Conclusion ] The mutant MsAcT (S11C) successfully achieved microbial enzymatic biosynthesis from THOP to N-ATHOP.
Publication Date
7-15-2025
First Page
85
Last Page
93
DOI
10.12441/spyswjs.20240320002
Recommended Citation
WEI, Chuanxiang; CHEN, Keru; HU, Yun; SUN, Chao; XUE, Rukang; SUN, Fubao; and CHENG, Jingfang
(2025)
"Synthesis of N-Acetyl- trans -4-hydroxyproline Catalyzed by Acyltransferase,"
Journal of Food Science and Biotechnology: Vol. 44:
Iss.
7, Article 9.
DOI: 10.12441/spyswjs.20240320002
Available at:
https://spsw.spyswjs.cnjournals.com/journal/vol44/iss7/9
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