Journal of Food Science and Biotechnology
Abstract
[Objective ] This study aims to investigate the in vitro antioxidant and anti-inflammatory activities of Yunnan tea flower extract (TFE ) and its Inhibitory effect on cell aging.[Method ] The main active components in TFE were determined by ultraviolet (UV)-visible spectrophotometry and high performance liquid chromatography (HPLC ).The antioxidant and anti-inflammatory activities of TFE were determined by the free radical scavenging assay and UV-induced photoaging model of human keratinocytes (HaCaT ),respectively.After the aging model of fibroblasts was established,the aging level of cells was assessed by the β-galactosidase (β-gal) staining assay,and the expression of matrix metalloproteinase- 1 (MMP- 1),matrix metalloproteinase- 3 (MMP- 3),and type Ⅰ procollagen (PC-1) were determined by enzyme-linked immunosorbent assay.[Result ] The mass fractions of total flavonoids,tea polyphenols,total proteins,and total sugars in TFE were 14.51%,12.83%,9.57%,and 50.34%,respectively.Ester-type catechins were the main tea polyphenols.The IC 50 in scavenging DPPH and ABTS free radicals were 34.32 µg/mL and 227.79 µg/mL,respectively.TFE significantly inhibited reactive oxygen species (ROS ) production-induced by UV in HaCaT cells,down-regulated the expression of IL-6 and TNF-α,up-regulated the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase- 1 (HO- 1).In addition,TFE inhibited the aging of fibroblasts,down-regulated the expression of MMP- 1 and MMP- 3,and up-regulated the expression of PC-1.[Conclusion ] TFE had strong antioxidant and anti-inflammatory activities.It can down-regulated the expression of MMP- 1 and MMP- 3 and up-regulated the expression of PC- 1 to prevent cell aging,demonstrating potential anti-aging activity.
Publication Date
7-15-2025
First Page
74
Last Page
84
DOI
10.12441/spyswjs.20241017002
Recommended Citation
YU, Shishuai; HAO, Yining; PENG, Bo; LIU, Youyun; and QU, Liping
(2025)
"In Vitro Antioxidant and Anti-inflammatory Activities of Tea Flower Extract and Its Inhibitory Effect on Cell Aging,"
Journal of Food Science and Biotechnology: Vol. 44:
Iss.
7, Article 8.
DOI: 10.12441/spyswjs.20241017002
Available at:
https://spsw.spyswjs.cnjournals.com/journal/vol44/iss7/8
References
［1］ 师梦楠，彭云，张杰，等.云南大叶种茶树花生化成分及体外抗氧化活性研究 ［J］.食品工业科技，2022，43（16）：298-306.SHI M N，PENG Y，ZHANG J，et al.Biochemical components and in vitro antioxidant activities of large-leaf species tea flowers in Yunnan ［J］.Science and Technology of Food Industry，2022，43（16）：298-306.（in Chinese ）
［2］ 黄阿根，董瑞建，韦红.茶树花活性成分的分析与鉴定 ［J］.食品科学，2007，28（7）：400-403.HUANG A G，DONG R J，WEI H.Identification and analysis of major bioactive compounds in tea flower ［J］.Food Science，2007，28（7）：400-403.（in Chinese ）
［3］ 李红蝶，肖田，李亦龙，等.茶树花的功能成分及相关产品研究进展 ［J］.食品安全质量检测学报，2024，15（6）：117-123.LI H D，XIAO T，LI Y L，et al.Research progress on functional components of Camellia sinensis and related products［J］.Journal of Food Safety & Quality，2024，15（6）：117-123.（in Chinese ）
［4］ 生吉萍，宿文凡，石家豪，等.茶树花黄酮类化合物研究进展［J］.食品安全质量检测学报，2022，13（4）：1019 -1026.SHENG J P，SU W F，SHI J H，et al.Research progress of flavonoids in tea flower ［J］.Journal of Food Safety & Quality，2022，13（4）：1019 -1026.（in Chinese ）
［5］ MATSUDA H，NAKAMURA S，MORIKAWA T，et al.New biofunctional effects of the flower buds of Camellia sinensis and its bioactive acylated oleanane-type triterpene oligoglycosides ［J］.Journal of Natural Medicines，2016，70（4）：689-701.
［6］ 罗珍，宁初光，李祚禛，等.番茄、野樱莓提取物抗光老化功效及机制研究 ［J］.食品工业科技，2023，44（16）：395-402.LUO Z，NING C G，LI Z Z，et al.Anti-photoaging effect and mechanism of tomato and Aronia melanocarpa extract［J］.Science and Technology of Food Industry，2023，44（16）：395-402.（in Chinese ）
［7］ 常子怡，王广莲，汤雯珺，等.光老化对皮肤角质形成细胞中转录组学相关基因表达的影响 ［J］.中国皮肤性病学杂志，2024，38（6）：612-619.CHANG Z Y，WANG G L，TANG W J，et al.Effect of photoaging on transcriptomic-related gene expression in skin keratinocytes ［J］.The Chinese Journal of Dermatovenereology，2024，38（6）：612-619.（in Chinese ）
［8］ 任倩倩，袁永雷，曲丽萍.影响皮肤光老化信号通路的植物提取物 ［J］.日用化学品科学，2023，46（3）：34-41.REN Q Q，YUAN Y L，QU L P.Plant extracts affecting skin photoaging signaling pathways ［J］.Detergent & Cosmetics，2023，46（3）：34-41.（in Chinese ）
［9］ OH J H，JOO Y H，KARADENIZ F，et al.Syringaresinol inhibits UVA-induced MMP- 1 expression by suppression of MAPK/AP- 1 signaling in HaCaT keratinocytes and human dermal fibroblasts ［J］.International Journal of Molecular Sciences，2020，21（11）：3981.
［10］ 陈誉，罗磊，王硕，等.咖啡果壳多酚的提取及抗氧化和抗炎特性 ［J］.食品与生物技术学报，2022，41（8）：104-111.CHEN Y，LUO L，WANG S，et al.Antioxidant and anti-inflammatory properties of polyphenols extracted from coffee cherry husks ［J］.Journal of Food Science and Biotechnology，2022，41（8）：104-111.（in Chinese ）
［11］ 张慧娥，杜连云，初孟瑶，等.人参叶通过抑制细胞活性氧的积累延缓皮肤衰老 ［J］.食品工业科技，2024，45（3）：328-337.ZHANG H E，DU L Y，CHU M Y，et al.Ginseng folium attenuates skin aging by limiting the accumulation of cellular reactive oxygen species ［J］.Science and Technology of Food Industry，2024，45（3）：328-337.（in Chinese ）
［12］ 蔡春梅，黄玉叶，李丽燕，等.黄芩提取物抗 UVA诱导NIH 3T3细胞光老化的作用 ［J］.食品工业科技，2024，45（12）：327-333.CAI C M，HUANG Y Y，LI L Y，et al.Scutellaria baicalensis extract inhibits the UVA-induced photoaging in NIH 3T3 cells［J］.Science and Technology of Food Industry，2024，45（12）：327-333.（in Chinese ）
［13］ 陈建芳，刘思思，张彤赫，等.银耳多糖的抗氧化活性及对成纤维细胞蛋白基因表达的影响 ［J］.现代食品科技，2024，40（7）：64-71.CHEN J F，LIU S S，ZHANG T H，et al.Antioxidant activity of Tremella polysaccharide and its effect on fibroblast protein gene expression ［J］.Modern Food Science and Technology，2024，40（7）：64-71.（in Chinese ）
［14］ 黄艳，商虎，朱嘉威，等.加工工艺对茶树花品质及抗氧化活性的影响 ［J］.食品科学，2020，41（11）：165-170.HUANG Y，SHANG H，ZHU J W，et al.Effects of processing treatments on quality and antioxidant activity of tea plant flower ［J］.Food Science，2020，41（11）：165-170.（in Chinese ）
［15］ QU L P，WANG F F，CHEN Y Y.Protective effect and mechanism research of Phyllanthus emblica Linn.fruit extract on UV-induced photodamage in keratinocytes ［J］.Photochemical & Photobiological Sciences，2023，22（8）：1945 -1959.
［16］ 朱恒杏，邵孟茹，洪天畅，等.黑枸杞花青素、EGCG对中波紫外线诱导 HaCaT细胞氧化损伤的协同保护作用［J］.现代食品科技，2024，40（8）：106-115.ZHU H X，SHAO M R，HONG T C，et al.Synergistic protective effects of the anthocyanins from black wolfberry and EGCG against the UVB-induced oxidative damage in HaCaT cells ［J］.Modern Food Science and Technology，2024，40（8）：106-115.（in Chinese ）
［17］ 武思敏，袁梅，满佳旭，等.绿原酸对 UVB损伤人永生化表皮角质细胞的保护作用 ［J］.食品与生物技术学报，2023，42（5）：96-101.WU S M，YUAN M，MAN J X，et al.Protective effect of chlorogenic acid on UVB damaged HaCaT cells ［J］.Journal of Food Science and Biotechnology，2023，42（5）：96-101.（in Chinese ）
［18］ 吴海英，黄倩倩，李诗华，等.金花茶花活性组分成分分析及抗炎作用 ［J］.食品与发酵工业，2024，50（8）：197-203.WU H Y，HUANG Q Q，LI S H，et al.Component analysis and anti-inflammatory activity of active ingredients from Camellia nitidissima Chi flower ［J］.Food and Fermentation Industries，2024，50（8）：197-203.（in Chinese ）
［19］ FIORELLI S，PORRO B，COSENTINO N，et al.Activation of Nrf 2/HO- 1 pathway and human atherosclerotic plaque vulnerability：an in vitro and in vivo study［J］.Cells，2019，8（4）：356.
［20］ NITURE S K，KHATRI R，JAISWAL A K.Regulation of Nrf2：an update ［J］.Free Radical Biology and Medicine，2014，66：36-44.
［21］ HU D，PAWLIKOWSKA L，KANAYA A，et al.Serum insulin -like growth factor- 1 binding proteins 1 and 2 and mortality in older adults：the health，aging，and body composition study ［J］.Journal of the American Geriatrics Society，2009，57（7）：1213 -1218.
［22］ CHAIPRASONGSUK A，PANICH U.Role of phytochemicals in skin photoprotection via regulation of Nrf2［J］.Frontiers in Pharmacology，2022，13：823881.
［23］ ZHOU J Z，CHEN F H，YAN A M，et al.Madecassoside protects retinal pigment epithelial cells against hydrogen peroxide-induced oxidative stress and apoptosis through the activation of Nrf 2/HO- 1 pathway ［J］.Bioscience Reports，2020，40（10）：BSR 20194347.
［24］ FULOP G A，KISS T，TARANTINI S，et al.Nrf2 deficiency in aged mice exacerbates cellular senescence promoting cerebrovascular inflammation ［J］.GeroScience，2018，40（5/6）：513-521.
［25］ SUN J M，LIU Y X，LIU Y D，et al.Salvianolic acid B protects against UVB-induced skin aging via activation of NRF 2［J］.Phytomedicine，2024，130：155676.
［26］ 张颖杰，王绫欢，马焱，等.EGCG通过 Nrf2/HO- 1信号通路延缓内皮细胞衰老的实验研究 ［J］.中国心血管病研究，2024，22（6）：571-576.ZHANG Y J，WANG L H，MA Y，et al.Experimental study on EGCG delaying endothelial cell senescence via Nrf2/HO- 1 signaling pathway ［J］.Chinese Journal of Cardiovascular Research，2024，22（6）：571-576.（in Chinese ）
［27］ 梅早菊，吴信浪，曲丽萍.云南腾冲牛至精油成分及其抗炎和抗衰老活性特征 ［J］.中国食品添加剂，2024，35（4）：192-200.MEI Z J，WU X L，QU L P.Components analysis of Origanum vulgare L.essential oil from Tengchong，Yunnan and its anti-inflammatory and anti-aging activities ［J］.China Food Additives，2024，35（4）：192-200.（in Chinese ）
［28］ HE M X，ZHOU P Y，CHEN H K，et al.（-）-α-Bisabolol inhibits D-Gal-induced HSF cellular senescence in vitro and prevents skin aging in vivo by reducing SASP ［J］.Iranian Journal of Basic Medical Sciences，2024，27（9）：1105 -1114.
［29］ BAEERI M，DIDARI T，NAVAEI-NIGJEH M，et al.Regulation of cellular aging in rat embryonic fibroblast cells using gallic acid ［J］.International Pharmacy Acta，2018，1（1），56-56.
［30］ LOO Y C，HU H C，YU S Y，et al.Development on potential skin anti-aging agents of Cosmos caudatus Kunth via inhibition of collagenase，MMP- 1 and MMP- 3 activities［J］.Phytomedicine，2023，110：154643.
［31］ MIRASTSCHIJSKI U，LUPŠE B，MAEDLER K，et al.Matrix metalloproteinase- 3 is key effector of TNF -α-induced collagen degradation in skin ［J］.International Journal of Molecular Sciences，2019，20（20）：5234.
［32］ ÅGREN M S，SCHNABEL R，CHRISTENSEN L H，et al.Tumor necrosis factor- α-accelerated degradation of type I collagen in human skin is associated with elevated matrix metalloproteinase （MMP）-1 and MMP- 3 ex vivo［J］.European Journal of Cell Biology，2015，94（1）：12-21.
［33］ OH J H，Nam G B，Karadeniz F，et al.Evaluation and enzyme-aided enhancement of anti-photoaging properties of Camellia japonica in UVA-irradiated keratinocytes ［J］.Zeitschrift für Naturforschung C，2022，77（7/8），287-296.
［34］ PARK H M，MOON E，KIM A J，et al.Extract of Punica granatum inhibits skin photoaging induced by UVB irradiation ［J］.International Journal of Dermatology，2010，49（3）：276-282.
［35］ LIU S W，YOU L，ZHAO Y X，et al.Hawthorn polyphenol extract inhibits UVB-induced skin photoaging by regulating MMP expression and type I procollagen production in mice ［J］.Journal of Agricultural and Food Chemistry，2018，66（32）：8537 -8546.
［36］ WLASCHEK M，MAITY P，MAKRANTONAKI E，et al.Connective tissue and fibroblast senescence in skin aging ［J］.Journal of Investigative Dermatology，2021，141（4）：985-992.
［37］ KIM M K，SHIN H S，SHIN M H，et al.Dual role of enhancer of zeste homolog 2 in the regulation of ultraviolet radiation-induced matrix metalloproteinase- 1 and type I procollagen expression in human dermal fibroblasts ［J］.Matrix Biology，2023，119：112-124.
［38］ HUANG Y H，WU P Y，WEN K C，et al.Protective effects and mechanisms of Terminalia catappa L.methenolic extract on hydrogen-peroxide-induced oxidative stress in human skin fibroblasts ［J］.BMC Complementary and Alternative Medicine，2018，18（1）：266.
［39］ BRAVO K，DUQUE L，FERRERES F，et al.Passiflora tarminiana fruits reduce UVB-induced photoaging in human skin fibroblasts ［J］.Journal of Photochemistry and Photobiology B：Biology，2017，168：78-88.