Reconstruction and Analysis of Genome-scale Metabolic Model of Aspergillus oryzae FMME-S- 38

Authors

Cong GAO, School of Biotechnology , Jiangnan University , Wuxi 214122 , China
Weijia YUAN, School of Biotechnology , Jiangnan University , Wuxi 214122 , China
Chao YE, School of Food Science and Pharmaceutical Engineering , Nanjing Normal University , Nanjing 210023 , China
Jia LIU, School of Biotechnology , Jiangnan University , Wuxi 214122 , China
Liming LIU, School of Biotechnology , Jiangnan University , Wuxi 214122 , ChinaFollow

Corresponding Author(s)

刘立明（1976—），男，博士，教授，博士研究生导师，主要从事微生物制造工程研究。E-mail：mingll@jiangnan.edu.cn

Abstract

[Objective ] A genome-scale metabolic model (GSMM ) of the high L-malic acid-producing strain Aspergillus oryzae (A.oryzae ) FMME-S- 38 was constructed and analyzed.[Method ] The new GSMM iJW1333 was established for A.oryzae FMME-S- 38 through a two-step process of draft reconstruction and model refinement.[Result ] The model integrated 1 723 biochemical reactions,1 723 metabolites,and 1 333 functional genes,achieving the prediction accuracy of 94.6% for substrate metabolism and 92.0% for cell growth.Comparative genomic analysis revealed that mutations in the genes involved in the hexose monophosphate pathway (HMP ) and oxidative phosphorylation underpinned the enhanced L-malic acid production of A.oryzae FMME-S- 38.Nitrogen source and cofactors (NADH and ATP ) were identified as key determinants of efficient L-malic acid biosynthesis.Reducing the mass concentration of nitrogen source and the level of dissolved oxygen (DO) attenuated HMP while strengthening the reductive tricarboxylic acid (rTCA ) branch,enabling A.oryzae FMME-S- 38 to achieve an L-malic acid titer of (154.2±1.8) g/L.[Conclusion ] This study clarifies the critical pathways and physiological mechanisms responsible for high L-malic acid production,providing a solid foundation for employing A.oryzae FMME-S- 38 in the biosynthesis of other valuable metabolites.

Publication Date

7-15-2025

First Page

21

Last Page

30

DOI

10.12441/spyswjs.20230706004

Recommended Citation

GAO, Cong; YUAN, Weijia; YE, Chao; LIU, Jia; and LIU, Liming (2025) "Reconstruction and Analysis of Genome-scale Metabolic Model of Aspergillus oryzae FMME-S- 38," Journal of Food Science and Biotechnology: Vol. 44: Iss. 7, Article 3.
DOI: 10.12441/spyswjs.20230706004
Available at: https://spsw.spyswjs.cnjournals.com/journal/vol44/iss7/3

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