Establishment and Application of a UPLC-MS/MS Method for Simultaneous Determination of 39 Veterinary Drugs and Their Metabolites in Black Bone Chicken

Authors

Jiawei DU, School of Animal Science and Technology , Ningxia University , Yinchuan 750021 , China
Yongqin LI, School of Animal Science and Technology , Ningxia University , Yinchuan 750021 , China ;Ningxia Veterinary Drugs and Fodder Inspection Institute , Yinchuan 750011 , China
Shuangyuan JIN, School of Animal Science and Technology , Ningxia University , Yinchuan 750021 , China
Xueyan WANG, School of Animal Science and Technology , Ningxia University , Yinchuan 750021 , China
Hongwu WEN, Husbandry Technology Extension Service Center of Yanchi Bureau of Agriculture and Rural Affairs , Wuzhong 751500 , China
Xuelian GUO, School of Animal Science and Technology , Ningxia University , Yinchuan 750021 , China
Hao LI, School of Animal Science and Technology , Ningxia University , Yinchuan 750021 , China
Lihua XU, School of Animal Science and Technology , Ningxia University , Yinchuan 750021 , ChinaFollow

Corresponding Author(s)

许立华（1972—），男，博士，教授，博士研究生导师，主要从事微生物学与免疫学、动物疫病诊断与防控技术研究。E-mail：littlezhe99@163.com

Abstract

[Objective ] This study aims to comprehensively investigate the risk of veterinary drug residues in black bone chicken.[Method ] A method for rapid and simultaneous analysis of 39 veterinary drugs and their metabolites in three categories was established based on low-temperature ultrasonic extraction,solid-phase extraction pretreatment,and ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS ).The samples were extracted by Mcllvaine-Na 2EDTA and phosphate buffer via ultrasonic treatment at low temperatures,purified by Oasis HLB solid-phase extraction column,separated by T 3 chromatographic column,and quantified by the matrix matching external standard method.[Result ] The 39 veterinary drugs and their metabolites showed good linear relationships in the matrix matching external standard solutions with the mass concentration range of 2.0~500.0 μg/L,with the correlation coefficients greater than 0.990.The limits of detection and quantification were 2.0 μg/kg and 10.0 μg/kg,respectively.The spike recovery rates of the method ranged from 68% to 111% at fortified mass fractions of 10.0~100.0 μg/kg,and the relative standard deviation (n=5) ranged from 0.65% to 15.12%.The method had high sensitivity and reliable qualitative and quantitative results,meeting the requirements for the detection and analysis of veterinary drug residues in black bone chicken.This method was then used to test 60 samples of black bone chicken sold on the market.The results showed that fluoroquinolones,tetracyclines,and sulfonamides were detected in commercial black bone chicken.The mass fraction of enrofloxacin,trimethoprim,and diaveridine exceeded the maximum residue limits in two (153.187,174.979 μg/kg ),two (55.007,111.391 μg/kg ),and two (153.779,189.801 μg/kg ) samples,respectively.[Conclusion ] There is a certain risk of the residues of sulfonamides in black bone chicken on the market,and it is necessary to strengthen the supervision on use of antibiotics in black bone chicken breeding.

Publication Date

7-15-2025

First Page

161

Last Page

172

DOI

10.12441/spyswjs.20240221001

Recommended Citation

DU, Jiawei; LI, Yongqin; JIN, Shuangyuan; WANG, Xueyan; WEN, Hongwu; GUO, Xuelian; LI, Hao; and XU, Lihua (2025) "Establishment and Application of a UPLC-MS/MS Method for Simultaneous Determination of 39 Veterinary Drugs and Their Metabolites in Black Bone Chicken," Journal of Food Science and Biotechnology: Vol. 44: Iss. 7, Article 16.
DOI: 10.12441/spyswjs.20240221001
Available at: https://spsw.spyswjs.cnjournals.com/journal/vol44/iss7/16

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