Health Risk Assessment of Multiple Mycotoxins in Rice , Maize , Soybean , and Wheat Flour Marketed in Shanghai

Authors

Zuoyin ZHU, The Institute for Agro-food Standards and Testing Technology , Shanghai Academy of Agricultural Sciences , Shanghai 201403 , China
Jie WANG, The Institute for Agro-food Standards and Testing Technology , Shanghai Academy of Agricultural Sciences , Shanghai 201403 , China
Junmei MA, Xining Agricultural Product Quality and Safety Testing Center , Xining 810003 , China
Hanke ZHAO, The Institute for Agro-food Standards and Testing Technology , Shanghai Academy of Agricultural Sciences , Shanghai 201403 , China
Haisheng CHENG, The Institute for Agro-food Standards and Testing Technology , Shanghai Academy of Agricultural Sciences , Shanghai 201403 , China
Chengbao HUANG, The Institute for Agro-food Standards and Testing Technology , Shanghai Academy of Agricultural Sciences , Shanghai 201403 , China
Junhua YANG, The Institute for Agro-food Standards and Testing Technology , Shanghai Academy of Agricultural Sciences , Shanghai 201403 , ChinaFollow

Corresponding Author(s)

杨俊花（1980—），女，研究员，硕士研究生导师，主要从事真菌毒素毒理学研究。E-mail：yangjunhua@saas.sh.cn

Abstract

[Objective ] This study aims to evaluate the potential health risks to consumers from exposure to multiple mycotoxins in grains.[Method ] Point and probabilistic assessment models were adopted to analyze the dietary exposure risks of adult residents in Shanghai to aflatoxins (AFs),ochratoxin A (OTA ),zearalenone (ZEN ),deoxynivalenols (DONs ),and fumonisins (FBs) through consumption of rice,maize,soybeans,and wheat flour.[Result ] The margin of exposure values (MoE ) for AFs in maize and aflatoxin G 1 (AFG 1) in soybeans exceeded 10 000,indicating no dietary exposure risk.In contrast,AFs in rice and wheat flour,as well as aflatoxin B 1 (AFB 1),aflatoxin B 2 (AFB 2),and aflatoxin G 2 (AFG 2) in soybeans,showed MoE below 10 000,suggesting a minor health risk.The estimated daily intake (EDI) values of ZEN,DONs,and FBs from the four grains were below the provisional maximum tolerable daily intake (PMTDI ),indicating no dietary risk.At the 95th percentile (P95) exposure level,the EDI of OTA from rice exceeded 100 ng/kg,while the risk quotient (RQ) of OTA in soybeans and DONs in wheat flour surpassed 100% under certain exposure scenarios,indicating a potential minor health risk.[Conclusion ] Overall,the exposure to AFs,OTA,ZEN,DONs,and FBs through consumption of four grains among adult residents in Shanghai was within safe limits.However,certain mycotoxins posed potential health risks under high-exposure conditions.These findings provide scientific basis for the risk management of mycotoxins in grains in Shanghai.

Publication Date

7-15-2025

First Page

125

Last Page

136

DOI

10.12441/spyswjs.20241202002

Recommended Citation

ZHU, Zuoyin; WANG, Jie; MA, Junmei; ZHAO, Hanke; CHENG, Haisheng; HUANG, Chengbao; and YANG, Junhua (2025) "Health Risk Assessment of Multiple Mycotoxins in Rice , Maize , Soybean , and Wheat Flour Marketed in Shanghai," Journal of Food Science and Biotechnology: Vol. 44: Iss. 7, Article 13.
DOI: 10.12441/spyswjs.20241202002
Available at: https://spsw.spyswjs.cnjournals.com/journal/vol44/iss7/13

References

［1］ 徐炜，张玉磊，施妍，等.真菌毒素降解酶及其在饲料与食品行业中的研究现状 ［J］.食品与生物技术学报，2023，42（1）：1-17.XU W，ZHANG Y L，SHI Y，et al.Studies on mycotoxin degrading enzymes and their applications in feed and food industries［J］.Journal of Food Science and Biotechnology，2023，42（1）：1-17.（in Chinese ）
［2］ LIM C W，YOSHINARI T，LAYNE J，et al.Multi-mycotoxin screening reveals separate occurrence of aflatoxins and ochratoxin a in Asian rice ［J］.Journal of Agricultural and Food Chemistry，2015，63（12）：3104 -3113.
［3］ SCHATZMAYR G，STREIT E.Global occurrence of mycotoxins in the food and feed chain：facts and figures［J］.World Mycotoxin Journal，2013，6（3）：213-222.
［4］ GRUBER-DORNINGER C，JENKINS T，SCHATZMAYR G.Global mycotoxin occurrence in feed：a ten-year survey［J］.Toxins，2019，11（7）：375.
［5］ 崔杰，王志强，李卫东.安徽省 2017—2021年谷物及其制品中真菌毒素污染现状研究 ［J］.安徽预防医学杂志，2024，30（3）：195-199.CUI J，WANG Z Q，LI W D.Study on mycotoxins pollution in cereals and their products in Anhui province from 2017 to 2021［J］.Anhui Journal of Preventive Medicine，2024，30（3）：195-199.（in Chinese ）
［6］ 李晓龙，吴丹，李英军，等.粮食中主要真菌毒素的调查分析与研究 ［J］.食品安全导刊，2022（32）：106-109.LI X L，WU D，LI Y J，et al.Analysis and research of main mycotoxins in grain ［J］.China Food Safety Magazine，2022（32）：106-109.（in Chinese ）
［7］ 朱佐银，赵含珂，程海生，等.2021—2022年上海地区大米、玉米、大豆和面粉中多种真菌毒素混合污染特征和交互作用分析 ［J］.中国农业科学，2024，57（12）：2454 -2466.ZHU Z Y，ZHAO H K，CHENG H S，et al.Study on characterization and interaction analysis of co-contamination of multi-mycotoxins in the flours of rice，maize，soybean and wheat flour in Shanghai from 2021 to 2022［J］.Scientia Agricultura Sinica，2024，57（12）：2454 -2466.（in Chinese ）
［8］ SCHÖNEBERG T，JENNY E，WETTSTEIN F E，et al.Occurrence of Fusarium species and mycotoxins in Swiss oats：impact of cropping factors ［J］.European Journal of Agronomy，2018，92：123-132.
［9］ TAVARES A，MENDONÇA I，LOURO H，et al.A contribution to hazard assessment of combined exposure to mycotoxins using in vitro toxicity testing ［J］.Toxicology Letters，2015，238（2）：S352-S353.
［10］ SCHOEMAN A，FLETT B C，JANSE VAN RENSBURG B.Evaluating three commonly used growth media for assessing fumonisin analogues FB 1，FB 2 and FB 3production by nine Fusarium verticillioides isolates［J］.Food Additives & Contaminants：Part A，2017，34（2）：291-298.
［11］ 叶永丽，张晓娟，唐莉莉，等.基于氧化应激和线粒体功能障碍的伏马毒素 B1诱导线虫神经毒性机制 ［J］.食品与生物技术学报，2024，43（3）：26-35.YE Y L，ZHANG X J，TANG L L，et al.Mechanism investigation of neurotoxicity of Caenorhabditis elegans induced by fumonaxin B 1-mediated oxidative stress and mitochondrial dysfunction ［J］.Journal of Food Science and Biotechnology，2024，43（3）：26-35.（in Chinese ）
［12］ 王向未，仇厚援，张志恒，等.食品中膳食暴露评估模型研究进展 ［J］.浙江农业学报，2012，24（4）：733-738.WANG X W，QIU H Y，ZHANG Z H，et al.Diet exposure assessment methods in food ［J］.Acta Agriculturae Zhejiangensis，2012，24（4）：733-738.（in Chinese ）
［13］ 丁小霞，李培武，白艺珍，等.中国花生黄曲霉毒素风险评估中膳食暴露非参数概率评估方法 ［J］.中国油料作物学报，2011，33（4）：402-408.DING X X，LI P W，BAI Y Z，et al.Non-parametric probability assessment of risk evaluation on dietary exposure to Chinese peanut aflatoxins ［J］.Chinese Journal of Oil Crop Sciences，2011，33（4）：402-408.（in Chinese）
［14］ 尹青春，陈小妹，周凌聿，等.龙眼及其制品中 44种真菌毒素高通量检测方法的建立及其膳食暴露风险评估［J］.现代食品科技，2024，40（7）：254-266.YIN Q C，CHEN X M，ZHOU L Y，et al.Development of a high-throughput detection assay and dietary exposure risk assessment for 44 mycotoxins in longan and its products［J］.Modern Food Science and Technology，2024，40（7）：254-266.（in Chinese ）
［15］ 郭耀东，陈璐，袁亚宏，等.基于概率分析的玉米食品中黄曲霉毒素 B1膳食暴露及风险评估 ［J］.食品科学，2013，34（11）：24-27.GUO Y D，CHEN L，YUAN Y H，et al.Dietary exposure and risk assessment of aflatoxin B 1 in corn-based foods in China using probabilistic approach ［J］.Food Science，2013，34（11）：24-27.（in Chinese ）
［16］ 李依玲，刘佳琳，黄娇，等.中国不同地区居民经小麦及其制品摄入脱氧雪腐镰刀菌烯醇暴露评估 ［J］.卫生研究，2022，51（5）：815-822，843.LI Y L，LIU J L，HUANG J，et al.Exposure assessment of deoxynivalenol in wheat and its products among residents in different regions of China ［J］.Journal of Hygiene Research，2022，51（5）：815-822，843.（in Chinese）
［17］ ZHU Z Y，GUO W B，CHENG H S，et al.Co-contamination and interactions of multiple mycotoxins and heavy metals in rice，maize，soybeans，and wheat flour marketed in Shanghai city ［J］.Journal of Hazardous Materials，2024，474：134695.
［18］ HAN Z，NIE D X，EDIAGE E N，et al.Cumulative health risk assessment of co-occurring mycotoxins of deoxynivalenol and its acetyl derivatives in wheat and maize：case study，Shanghai，China［J］.Food and Chemical Toxicology，2014，74：334-342.
［19］ 吴永宁，赵云峰，李敬光.第五次中国总膳食研究 ［M］.北京：科学出版社，2018：31-32.
［20］ 李莉，李硕，王海燕，等.食品中致癌性生物毒素检测标准概述［J］.食品工业科技，2019，40（13）：310-315，321.LI L，LI S，WANG H Y，et al.Review of standard dectection methods of carcinogenic biotoxins in foods ［J］.Science and Technology of Food Industry，2019，40（13）：310-315，321.（in Chinese ）
［21］ BENFORD D，BOLGER P M，CARTHEW P，et al.Application of the margin of exposure （MoE） approach to substances in food that are genotoxic and carcinogenic［J］.Food and Chemical Toxicology，2010，48：S2-S24.
［22］ 贺月.上海市干湿沉降中多环芳烃赋存特征、溯源和健康风险研究 ［D］.上海：华东师范大学，2022.
［23］ 李丽娜.上海市多介质环境中持久性毒害污染物的健康风险评价 ［D］.上海：华东师范大学，2007.
［24］ BOOBIS A，FLARI V，GOSLING J P，et al.Interpretation of the margin of exposure for genotoxic carcinogens：elicitation of expert knowledge about the form of the dose response curve at human relevant exposures ［J］.Food and Chemical Toxicology，2013，57：106-118.
［25］ 贾韶蔚，王丰舟，过治勇，等.开封市 2015—2020年部分市售食品中黄曲霉毒素污染状况调查及膳食暴露风险评估［J］.保健医学研究与实践，2022，19（10）：6-10，24.JIA S W，WANG F Z，GUO Z Y，et al.Investigation of aflatoxin contamination status and dietary exposure risk assessment in some commercially available foods in Kaifeng from 2015 to 2020［J］.Health Medicine Research and Practice，2022，19（10）：6-10，24.（in Chinese ）
［26］ 韩德娟，李凤玲，李晋.2021年泰安市粮油食品中黄曲霉毒素 B1污染状况及膳食暴露风险评估 ［J］.中国油脂，2023，48（7）：73-78.HAN D J，LI F L，LI J.Risk assessment of aflatoxin B 1 pollution status and dietary exposure in grain and oil food in Tai’an in 2021［J］.China Oils and Fats，2023，48（7）：73-78.（in Chinese ）
［27］ 石萌萌，梁江，赵鹏，等.广西居民膳食中黄曲霉毒素暴露风险评估 ［J］.中国食品卫生杂志，2020，32（4）：432-436.SHI M M，LIANG J，ZHAO P，et al.Dietary exposure assessment of aflatoxins of residents in Guangxi ［J］.Chinese Journal of Food Hygiene，2020，32（4）：432-436.（in Chinese ）
［28］ ZHANG W W，LIU Y F，LIANG B H，et al.Probabilistic risk assessment of dietary exposure to aflatoxin B 1 in Guangzhou，China［J］.Scientific Reports，2020，10：7973.
［29］ NUGRAHA A，KHOTIMAH K，RIETJENS I M C M.Risk assessment of aflatoxin B 1 exposure from maize and peanut consumption in Indonesia using the margin of exposure and liver cancer risk estimation approaches ［J］.Food and Chemical Toxicology，2018，113：134-144.
［30］ HUONG B T M，TUYEN L D，MADSEN H，et al.Total dietary intake and health risks associated with exposure to aflatoxin B 1，ochratoxin a and fuminisins of children in Lao Cai province，Vietnam［J］.Toxins，2019，11（11）：638.
［31］ 李文廷，申颖，李洁，等.2019年云南市售大米真菌毒素污染水平及风险评估 ［J］.食品安全质量检测学报，2021，12（14）：5861 -5869.LI W T，SHEN Y，LI J，et al.Contamination level and risk assessment of mycotoxin in rice sold in Yunnan province in 2019［J］.Journal of Food Safety & Quality，2021，12（14）：5861 -5869.（in Chinese ）
［32］ 赵毕，吴平谷，章荣华，等.浙江省 5大类食物中赭曲霉毒素 A暴露评估 ［J］.中国食品卫生杂志，2022，34（2）：315-321.ZHAO B，WU P G，ZHANG R H，et al.Ochratoxin A dietary exposure assessment in five food categories from Zhejiang province ［J］.Chinese Journal of Food Hygiene，2022，34（2）：315-321.（in Chinese ）
［33］ 孟繁磊，范宏，谭莉，等.吉林省玉米真菌毒素污染状况及其膳食风险评估研究 ［J］.玉米科学，2021，29（5）：88-94.MENG F L，FAN H，TAN L，et al.Contamination status and dietary risk assessment of corn mycotoxins in Jilin province［J］.Journal of Maize Sciences，2021，29（5）：88-94.（in Chinese ）
［34］ 殷芳媛，田明胜，王李伟，等.上海市居民小麦粉中脱氧雪腐镰刀菌烯醇及其乙酰衍生物的暴露评估 ［J］.卫生研究，2015，44（4）：661-665.YIN F Y，TIAN M S，WANG L W，et al.Dietary exposure assessment of deoxynivalenol and its acetylated derivatives in wheat flour in Shanghai ［J］.Journal of Hygiene Research，2015，44（4）：661-665.（in Chinese ）
［35］ 王伟，邵兵，朱江辉，等.中国谷物制品中重要镰刀菌毒素膳食暴露评估研究 ［J］.卫生研究，2010，39（6）：709-714.WANG W，SHAO B，ZHU J H，et al.Dietary exposure assessment of some important Fusarium toxins in cereal-based products in China ［J］.Journal of Hygiene Research，2010，39（6）：709-714.（in Chinese ）
［36］ 郭耀东，刘艺茹，袁亚宏，等.我国主要食品中伏马菌素污染水平分析与风险评估 ［J］.西北农林科技大学学报（自然科学版 ），2014，42（1）：78-82，88.GUO Y D，LIU Y R，YUAN Y H，et al.Fumonisins contamination in major food products and its dietary risk assessment in China ［J］.Journal of Northwest A & F University （Natural Science Edition ），2014，42（1）：78-82，88.（in Chinese ）
［37］ 胡海生，高洁，杨厚兵，等.粮食中真菌毒素及其代谢物 的 产 生 和 影 响 ［J］.食 品 安 全 导 刊，2024（8）：167-169.HU H S，GAO J，YANG H B，et al.Production and influence of mycotoxins and their metabolites in grain ［J］.China Food Safety Magazine，2024（8）：167-169.（in Chinese）