Construction of Staphylococcus aureus Detection Method Based on

Authors

Silver Nanoclusters DNA, School of Food Science and Engineering , Tianjin University of Science and Technology , Tianjin 300457 , China
Xiaotong LU, School of Food Science and Engineering , Tianjin University of Science and Technology , Tianjin 300457 , China
Jinzhao LI, School of Food Science and Engineering , Tianjin University of Science and Technology , Tianjin 300457 , China
Xinjun DU, School of Food Science and Engineering , Tianjin University of Science and Technology , Tianjin 300457 , ChinaFollow

Corresponding Author(s)

康青（1990—），女，博士，讲师，硕士研究生导师，主要从事食品安全相关研究。E-mail：kangqing@tust.edu.cn

Abstract

[Objective ] A simple and sensitive detection method for Staphylococcus aureus is established for the efficient detection of this pathogen in food.[Method ] Targeting Staphylococcus aureus,a simple and sensitive fluorescent detection method for Staphylococcus aureus,EXPAR-P-AgNCs,was developed based on exponential amplification reaction (EXPAR ) combined with DNA silver nanoclusters (P-AgNCs ).A recognition probe was designed to initiate EXPAR in the presence of Staphylococcus aureus,generating a large amount of target DNA(T).The binding of T to hairpin P displaced the G-rich strand from P-AgNCs,resulting in a decrease in the relative fluorescence intensity of P-AgNCs and enabling the detection of Staphylococc us aureus.[Result ] The relative fluorescence intensity showed a good linear relationship with Staphylococc us aureus concentration in the range of 1×10² and 1×10⁶ CFU/mL.The limit of detection was as low as 10 CFU/mL.The method shows high specificity without cross-reactions.The recovery rates for Staphylococcus aureus spiked in milk and pork samples ranged from 94.5% to 115.0%.[Conclusion ] The developed detection method demonstrates high sensitivity and excellent specificity,offering a promising approach for the detection of microbial pathogens or other contaminants threatening food safety.

Publication Date

6-15-2025

First Page

144

Last Page

152

DOI

10.12441/spyswjs.20240905001

Recommended Citation

DNA, Silver Nanoclusters; LU, Xiaotong; LI, Jinzhao; and DU, Xinjun (2025) "Construction of Staphylococcus aureus Detection Method Based on," Journal of Food Science and Biotechnology: Vol. 44: Iss. 6, Article 34.
DOI: 10.12441/spyswjs.20240905001
Available at: https://spsw.spyswjs.cnjournals.com/journal/vol44/iss6/34

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