Rapid Determination of Vitamin B 1 in Infant Formula Milk Powder by Fluorescent Microsphere Immunochromatographic Assay

Authors

Qianqian LU, Wuxi Food Safety Inspection and Test Center , Wuxi 214142 , China ;Technology Innovation Center of Special Food for State Market Regulation , Wuxi 214142 , China ;School of Food Science and Technology , Jiangnan University , Wuxi 214122 , China
Ting YANG, Wuxi Food Safety Inspection and Test Center , Wuxi 214142 , China ;Technology Innovation Center of Special Food for State Market Regulation , Wuxi 214142 , China
Yongwei FENG, Wuxi Food Safety Inspection and Test Center , Wuxi 214142 , China ;Technology Innovation Center of Special Food for State Market Regulation , Wuxi 214142 , China
Jialin HU, School of Food Science and Technology , Jiangnan University , Wuxi 214122 , China
Hua KUANG, School of Food Science and Technology , Jiangnan University , Wuxi 214122 , China ;State Key Laboratory of Food Science and Resources , Jiangnan University , Wuxi 214122 , China
Chuanlai XU, School of Food Science and Technology , Jiangnan University , Wuxi 214122 , China
Lingling GUO, School of Food Science and Technology , Jiangnan University , Wuxi 214122 , ChinaFollow

Corresponding Author(s)

郭玲玲（1988—），女，博士，助理研究员，主要从事食品安全快速检测研究。E-mail：gling0329@jiangnan.edu.cn

Abstract

[Objective ] A fluorescent immunochromatographic assay was established based on vitamin B 1 monoclonal antibody to measure the vitamin B 1 in infant formula milk powder.[Method ] The performance of vitamin B 1 monoclonal antibody was evaluated.On this basis,a highly sensitive fluorescent immunochromatographic test strip was developed.[Result ] The vitamin B 1 monoclonal antibody showed high sensitivity,affinity,and specificity.The fluorescent immunochromatographic test strip showed a visual limit of detection (vLOD ) of 10 μg/kg,a cut-off value of 200 μg/kg,and a calculated limit of detection (cLOD ) of 2.056 μg/kg.In addition,the recovery rate of this method was 97.3%~115.4%.The test results of the established method were consistent with the liquid chromatography-tandem mass spectrometry (LC-MS/MS ) results,with the relative standard deviation (RSD ) less than 10%,which indicated good accuracy and stability.[Conclusion ] The fluorescent immunochromatographic assay can be used for the rapid detection of actual infant formula milk powder samples.It does not require large-scale instruments or equipment and is portable,thus being of great significance for the rapid screening of a large number of samples.

Publication Date

6-15-2025

First Page

106

Last Page

114

DOI

10.12441/spyswjs.20240409004

Recommended Citation

LU, Qianqian; YANG, Ting; FENG, Yongwei; HU, Jialin; KUANG, Hua; XU, Chuanlai; and GUO, Lingling (2025) "Rapid Determination of Vitamin B 1 in Infant Formula Milk Powder by Fluorescent Microsphere Immunochromatographic Assay," Journal of Food Science and Biotechnology: Vol. 44: Iss. 6, Article 30.
DOI: 10.12441/spyswjs.20240409004
Available at: https://spsw.spyswjs.cnjournals.com/journal/vol44/iss6/30

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