Engineering Escherichia coli Tolerance to Improve Succinic Acid Production

Authors

Jingyu PAN, School of Biotechnology , Jiangnan University , Wuxi 214122 , China
Yongsheng TANG, School of Biotechnology , Jiangnan University , Wuxi 214122 , China
Jia LIU, School of Biotechnology , Jiangnan University , Wuxi 214122 , China
Cong GAO, School of Biotechnology , Jiangnan University , Wuxi 214122 , China
Xiaomin LI, School of Biotechnology , Jiangnan University , Wuxi 214122 , China
Wei SONG, School of Life Sciences and Health Engineering , Jiangnan University , Wuxi 214122 , China
Wanqing WEI, School of Biotechnology , Jiangnan University , Wuxi 214122 , China
Guipeng HU, School of Life Sciences and Health Engineering , Jiangnan University , Wuxi 214122 , China
Jing WU, School of Life Sciences and Health Engineering , Jiangnan University , Wuxi 214122 , China
Liming LIU, School of Biotechnology , Jiangnan University , Wuxi 214122 , China
Xiulai CHEN, School of Biotechnology , Jiangnan University , Wuxi 214122 , ChinaFollow

Corresponding Author(s)

陈修来（1985—），男，博士，教授，博士研究生导师，主要从事系统代谢工程与合成生物学研究。E-mail：xlchen@jiangnan.edu.cn

Abstract

[Objective ] This study aims to enhance the high osmotic pressure tolerance of Escherichia coli (E.coli ) for the efficient production of succinic acid.[Method ] Adaptive evolution,transcriptomic analysis,reverse metabolic engineering,and fermentation optimization were employed for improving E.coli tolerance to high osmotic pressure.[Result ] By analyzing the key factors influencing succinic acid production by fermentation,osmotic pressure was identified as the critical factor reducing succinic acid production efficiency.A mutant strain,E.coli FN-6,with tolerance to high osmotic pressure was obtained through adaptive evolution.The key gene cusC responsible for osmotic pressure tolerance was identified by transcriptomic analysis.After optimization of the expression level of cusC,the engineered strain E.coli FN- 20 showed strong tolerance to high osmotic pressure.In a 5 L fermenter with E.coli FN- 20,the succinic acid titer,yield,and productivity were up to 145.27 g/L,90.0%,and 2.01 g/(L·h),respectively.[Conclusion ] The obtained E.coli FN-20 strain with tolerance to high osmotic pressure enabled efficient production of succinic acid.This provides a new approach for the industrial production of succinic acid and provides the reference for producing other high value-added chemicals by fermentation.

Publication Date

6-15-2025

First Page

66

Last Page

77

DOI

10.12441/spyswjs.20240223004

Recommended Citation

PAN, Jingyu; TANG, Yongsheng; LIU, Jia; GAO, Cong; LI, Xiaomin; SONG, Wei; WEI, Wanqing; HU, Guipeng; WU, Jing; LIU, Liming; and CHEN, Xiulai (2025) "Engineering Escherichia coli Tolerance to Improve Succinic Acid Production," Journal of Food Science and Biotechnology: Vol. 44: Iss. 6, Article 26.
DOI: 10.12441/spyswjs.20240223004
Available at: https://spsw.spyswjs.cnjournals.com/journal/vol44/iss6/26

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