Journal of Food Science and Biotechnology
Abstract
[Objective ] The aim of this study is to synthesize aminated glycogen (NG) for loading of the photosensitizer TTVBA and construct a nano drug delivery system (NGT NPs ) for photodynamic therapy (PDT ) of tumors.[Method ] Positively charged NG was synthesized through modification of glycogen with diethylenetriamine (DETA ).Then,TTVBA was encapsulated by NG via electrostatic interaction to prepare NGT NPs.The prepared NGT NPs were characterized by infrared spectroscopy,dynamic light scattering (DLS ),and transmission electron microscopy (TEM ).The hemolysis assay was performed to evaluate the biocompatibility of NGT NPs.The reactive oxygen species (ROS ) generation capability of cells treated with NGT NPs was detected with the DCFH-DA probe.The in vitro antitumor activity of NGT NPs was assessed by the CCK- 8 assay and AM/PI staining.[Result ] NGT NPs were spherical,with the diameter of 104.8 nm and the surface potential of 23.83 mV.The ROS generated by the cells treated with NGT NPs (20 μg/mL ) under light was 12 folds of that in the dark.The NGT NPs group possessed a hemolysis rate below 0.41%,which indicated excellent biocompatibility.In B16 cells under light,NGT NPs raised the ROS level (P<0.001) while reducing the cell survival rate in a mass concentration-dependent manner.AM/PI staining showed more cell death in the light group than in the control group.[Conclusion ] NGT NPs possessed good biocompatibility,light-responsive induction of ROS generation,and antitumor activity.This work provides a novel and efficient nanocarrier for the PDT of tumors.
Publication Date
6-15-2025
First Page
50
Last Page
58
DOI
10.12441/spyswjs.20230319003
Recommended Citation
MA, Keke; FANG, Jiurui; ZHANG, Huijie; and CHEN, Jinghua
(2025)
"Photosensitizer-loaded Glycogen for Photodynamic Therapy of Tumor,"
Journal of Food Science and Biotechnology: Vol. 44:
Iss.
6, Article 24.
DOI: 10.12441/spyswjs.20230319003
Available at:
https://spsw.spyswjs.cnjournals.com/journal/vol44/iss6/24
References
［1］ SHELEG S V，ZHAVRID E A，KHODINA T V，et al.Photodynamic therapy with chlorin e 6 for skin metastases of melanoma ［J］.Photodermatology，Photoimmunology & Photomedicine，2004，20（1）：21-26.
［2］ DAI J，WU M，WANG Q，et al.Red blood cell membrane-camouflaged nanoparticles loaded with AIEgen and Poly （I∶C） for enhanced tumoral photodynamic-immunotherapy ［J］.National Science Review，2021，8（6）：nwab 039.
［3］ CORREIA J H，RODRIGUES J A，PIMENTA S，et al.Photodynamic therapy review：principles，photosensitizers，applications，and future directions ［J］.Pharmaceutics，2021，13（9）：1332.
［4］ KWIATKOWSKI S，KNAP B，PRZYSTUPSKI D，et al.Photodynamic therapy-mechanisms，photosensitizers and combinations ［J］.Biomedicine & Pharmacotherapy，2018，106：1098 -1107.
［5］ GAI S L，YANG G X，YANG P P，et al.Recent advances in functional nanomaterials for light-triggered cancer therapy［J］.Nano Today，2018，19：146-187.
［6］ MASTER A，LIVINGSTON M，SEN GUPTA A.Photodynamic nanomedicine in the treatment of solid tumors：perspectives and challenges ［J］.Journal of Controlled Release，2013，168（1）：88-102.
［7］ XIAO Q Q，LI X T，LI Y，et al.Biological drug and drug delivery-mediated immunotherapy ［J］.Acta Pharmaceutica Sinica B，2021，11（4）：941-960.
［8］ SAHDEV P，OCHYL L J，MOON J J.Biomaterials for nanoparticle vaccine delivery systems ［J］.Pharmaceutical Research，2014，31（10）：2563 -2582.
［9］ HU X K，YANG G Y，CHEN S，et al.Biomimetic and bioinspired strategies for oral drug delivery ［J］.Biomaterials Science，2020，8（4）：1020 -1044.
［10］ HAN Y N，HU B，WANG M Y，et al.pH-Sensitive tumor-targeted hyperbranched system based on glycogen nanoparticles for liver cancer therapy ［J］.Applied Materials Today，2020，18：100521.
［11］ MELÉNDEZ R，MELÉNDEZ-HEVIA E，CANELA E I.The fractal structure of glycogen：a clever solution to optimize cell metabolism ［J］.Biophysical Journal，1999，77（3）：1327 -1332.
［12］ MORRIS G A，ANG S，HILL S E，et al.Molar mass and solution conformation of branched α（1→4），α（1→6） glucans.Part I：glycogens in water ［J］.Carbohydrate Polymers，2008，71（1）：101-108.
［13］ 张成，吕雪芹，金柯，等.改造细胞质膜促进途径酶组装及N-乙酰氨基葡萄糖合成 ［J］.食品与生物技术学报，2021，40（10）：7-15.ZHANG C，LYU X Q，JIN K，et al.Engineering plasma membrane to promote enzyme assembly and N-acetylglucosamine synthesis ［J］.Journal of Food Science and Biotechnology，2021，40（10）：7-15.（in Chinese）
［14］ BESFORD Q A，CAVALIERI F，CARUSO F.Glycogen as a building block for advanced biological materials ［J］.Advanced Materials，2020，32（18）：1904625.
［15］ PERRONE M，LOPALCO A，LOPEDOTA A，et al.Preactivated thiolated glycogen as mucoadhesive polymer for drug delivery ［J］.European Journal of Pharmaceutics and Biopharmaceutics，2017，119：161-169.
［16］ ENGELBERTH S A，HEMPEL N，BERGKVIST M.Cationic dendritic starch as a vehicle for photodynamic therapy and siRNA co-delivery ［J］.Journal of Photochemistry and Photobiology B-Biology，2017，168：185-192.
［17］ SULLIVAN M A，VILAPLANA F，CAVE R A，et al.Nature of α and β particles in glycogen using molecular size distributions ［J］.Biomacromolecules，2010，11（4）：1094 -1100.
［18］ 邓淑豪，赖莉，王志清，等.糖原作为新型基因载体的 研 究［J］.食 品 与 生 物 技 术 学 报，2022，41（5）：38-45.DENG S H，LAI L，WANG Z Q，et al.Glycogen as novel gene vector ［J］.Journal of Food Science and Biotechnology，2022，41（5）：38-45.（in Chinese ）
［19］ 孙嘉笛，高璐，张银志，等.基于毒性效应通路荧光细胞传感模型的 T-2毒素毒性筛查 ［J］.食品与生物技术学报，2021，40（12）：35-43.SUN J D，GAO L，ZHANG Y Z，et al.T-2 toxicity screening based on fluorescence cell sensing model via toxic effect pathway ［J］.Journal of Food Science and Biotechnology，2021，40（12）：35-43.（in Chinese ）
［20］ WANG H H，PAN X H，WANG Y Q，et al.A new class of nitrobenzoic acid-based AIE photosensitizers for highly efficient photodynamic antibacterial therapy ［J］.Science China Materials，2021，64（10）：2601 -2612.