Journal of Food Science and Biotechnology
Abstract
[Objective ] This study aims to improve the detection specificity and sensitivity for Escherichia coli (E.coli ) O157:H7.[Method ] A high ly specific and sensitive method for the detection of E.coli O157:H7 was established based on magnetic separation and signal amplification strategies combined with surface-enhanced Raman spectroscopy (SERS ).Aptamers were used as recognition elements and combined with magnetic beads and polystyrene (PS) microspheres to construct a separation probe (MB@Apt ) and a signal amplification Raman tag (4-MBN@PS@Apt ),respectively.[Result ] In the presence of E.coli O157:H7,MB@Apt and 4-MBN@PS@Apt formed a “sandwich ” structured composite (MB@Apt/ E.coli O157:H7/4-MBN@PS@Apt ),which could be rapidly separated and enriched by an external magnet.After tetrahydrofuran (THF ) was added,the low biological background Raman reporter 4-MBN in the Raman tag was quickly released.The relative Raman intensity of 4-MBN at 1 073.9 cm-1 showed a good linear relationship with the logarithm of E.coli O157:H7 concentration in the range of 1×103 to 1×108 CFU/mL,with a determination coefficient (R2) of 0.98.The established method showed a limit of detection (LOD ) of 312 CFU/mL for E.coli O157:H7,the recovery rates ranging from 61.87% to 135.55% in tap water and milk samples,and relative standard deviations (RSD ) between 0.27% and 5.28%.[Conclusion ] This method exhibited high detection sensitivity for E.coli O157:H7 and provided a new approach for rapid detection of foodborne pathogenic bacteria.
Publication Date
6-15-2025
First Page
97
Last Page
105
DOI
10.12441/spyswjs.20230705003
Recommended Citation
WANG, Panxue; LI, Cen; LI, Xiang; LIU, Ying; and LI, Guoliang
(2025)
"Establishment of Surface-enhanced Raman Spectroscopy for Detection of Escherichia coli O157:H7,"
Journal of Food Science and Biotechnology: Vol. 44:
Iss.
6, Article 11.
DOI: 10.12441/spyswjs.20230705003
Available at:
https://spsw.spyswjs.cnjournals.com/journal/vol44/iss6/11
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