Effects of Palmitic Acid-rich Diet on Tumor Growth and Epithelial-mesenchymal Transition in the Mouse Model of Prostate Cancer

Authors

Jiayao CHEN, Wuxi School of Medicine , Jiangnan University , Wuxi 214122 , China
Fengjiao HUANG, Shantou Inspection and Testing Center , Shantou 515031 , China
Bingqian SUN, Wuxi School of Medicine , Jiangnan University , Wuxi 214122 , China
Yongquan CHEN, Wuxi School of Medicine , Jiangnan University , Wuxi 214122 , China
Xiaoying WANG, Wuxi School of Medicine , Jiangnan University , Wuxi 214122 , ChinaFollow

Corresponding Author(s)

王小英（1982—），女，博士，副教授，硕士研究生导师，主要从事脂质代谢与肿瘤发生、肿瘤免疫微环境的关系及相关机制研究。E-mail：xiaoyingwang@jiangnan.edu.cn

Abstract

[Objective ] This study aims to explore the effect of palmitic acid (PA)-rich diet on prostate cancer (PCa) in mice.[Method ] The TCGA database and immunohistochemistry (IHC) were used to analyze the differential expression of fatty acid synthase (FASN ) between the PCa tissue and adjacent normal tissue.In addition,twelve nude mice were subcutaneously implanted with LNCaP cells and assigned into two groups.One group was fed a PA-rich diet,while the other group was fed a normal control (NC) diet.Twelve mice overexpressing the C-myc gene (C-mycT) were assigned into two groups and fed the same diets as the nude mice.The growth of PCa was analyzed by tumor mass measurement,HE staining,and Ki 67 staining.Furthermore,the expression of epithelial-mesenchymal transition (EMT )-related proteins,such as E-cadherin,N-cadherin,and transforming growth factor (TGF )-β1,was determined by IHC and Western blot.Finally,relevant gene data were retrieved from the TCGA database and analyzed to explore the relationship among CD 36,TGF- β1,and PCa.[Result ] The expression of FASN was markedly higher in the PCa tissue than in the adjacent normal tissue,and there was a positive correlation between FASN expression and the progression of PCa.In nude mice and C-mycT mice,a PA-rich diet significantly promoted the progression of PCa,induced the EMT process,and activated the TGF- β1 signal.The expression of TGF- β1 was significantly associated with the decreased survival rate of individuals diagnosed with PCa.The expression of CD 36 was positively correlated with TGF- β1.Highly expressed TGF- β1,TGF- βR1,and CD 36 were risk factors for PCa,and TGF- β1 was an independent risk factor for PCa.[Conclusion ] The PA-rich diet promoted the tumor growth,activated the TGF- β1 signal pathway,and induced EMT in the mouse model of PCa.

Publication Date

5-15-2025

First Page

78

Last Page

86

DOI

10.12441/spyswjs.20230316001

Recommended Citation

CHEN, Jiayao; HUANG, Fengjiao; SUN, Bingqian; CHEN, Yongquan; and WANG, Xiaoying (2025) "Effects of Palmitic Acid-rich Diet on Tumor Growth and Epithelial-mesenchymal Transition in the Mouse Model of Prostate Cancer," Journal of Food Science and Biotechnology: Vol. 44: Iss. 5, Article 9.
DOI: 10.12441/spyswjs.20230316001
Available at: https://spsw.spyswjs.cnjournals.com/journal/vol44/iss5/9

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