PhrG-RapG-DegU Quorum Sensing Regulates Menaquinone- 7 Production in Bacillus subtilis

Authors

Junbao HUANG, College of Biological and Food Engineering , Anhui Polytechnic University , Wuhu 241000 , China
Xilin HUANG, College of Biological and Food Engineering , Anhui Polytechnic University , Wuhu 241000 , China
Yu CHEN, College of Biological and Food Engineering , Anhui Polytechnic University , Wuhu 241000 , China
Xuli GAO, College of Biological and Food Engineering , Anhui Polytechnic University , Wuhu 241000 , China
Yani LUO, College of Biological and Food Engineering , Anhui Polytechnic University , Wuhu 241000 , China
Wei TAO, College of Biological and Food Engineering , Anhui Polytechnic University , Wuhu 241000 , China
Yongyuan LIU, College of Biological and Food Engineering , Anhui Polytechnic University , Wuhu 241000 , China
Mingyu GUO, College of Biological and Food Engineering , Anhui Polytechnic University , Wuhu 241000 , China
Jing WU, College of Biological and Food Engineering , Anhui Polytechnic University , Wuhu 241000 , China
Yan LIU, College of Biological and Food Engineering , Anhui Polytechnic University , Wuhu 241000 , ChinaFollow

Corresponding Author(s)

刘艳（1981—），女，博士，教授，博士研究生导师，主要从事工业微生物代谢调控研究。E-mail：liuyan@ahpu.edu.cn

Abstract

[Objective ] The effects of the transcription regulation system on menaquinone- 7 (MK- 7) production in Bacillus subtilis (B.subtilis ) 168 were studied by excavating transcription regulators.[Method ] With the signal molecule PhrG pentapeptide,RapG,and the transcription regulator DegU,a PhrG-RapG-DegU regulatory system was successfully constructed in B.subtilis 168 to fine-tune the expression of key genes,so that the expression of key genes in the MK- 7 synthesis pathway can respond to the changes in cell density.[Result ] The production of MK- 7 in strain BS 06 reached 102.47 mg/L through this system.[Conclusion ] Fine tuning of quorum sensing can effectively improve the MK- 7 production in B.subtilis 168.

Publication Date

5-15-2025

First Page

50

Last Page

62

DOI

10.12441/spyswjs.20231130002

Recommended Citation

HUANG, Junbao; HUANG, Xilin; CHEN, Yu; GAO, Xuli; LUO, Yani; TAO, Wei; LIU, Yongyuan; GUO, Mingyu; WU, Jing; and LIU, Yan (2025) "PhrG-RapG-DegU Quorum Sensing Regulates Menaquinone- 7 Production in Bacillus subtilis," Journal of Food Science and Biotechnology: Vol. 44: Iss. 5, Article 6.
DOI: 10.12441/spyswjs.20231130002
Available at: https://spsw.spyswjs.cnjournals.com/journal/vol44/iss5/6

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