Nutritional Components and Immunoregulatory Mechanisms of Lactoferrin Formula

Authors

Mengying ZHOU, College of Biotechnology and Bioengineering , Zhejiang University of Technology , Hangzhou 310014 , China
Liujie ZHENG, College of Biotechnology and Bioengineering , Zhejiang University of Technology , Hangzhou 310014 , China
Ruiqing YAO, Hangzhou Qiandaohu Canobank Health Products Co ., Ltd., Hangzhou 311701 , China
Hongyu WU, Hangzhou Qiandaohu Canobank Health Products Co ., Ltd., Hangzhou 311701 , China
Gangliang HU, Hangzhou Qiandaohu Canobank Health Products Co ., Ltd., Hangzhou 311701 , China
Zhengquan HUO, Zhejiang Zh ongmengchang Health Technology Co ., Ltd., Hangzhou 310015 , China
Yinhua NI, College of Biotechnology and Bioengineering , Zhejiang University of Technology , Hangzhou 310014 , ChinaFollow

Corresponding Author(s)

倪银华（1985—），男，博士，副教授，博士研究生导师，主要从事生物活性物质开发和慢病防治研究。E-mail：shali0145@zjut.edu.cn

Abstract

[Objective ] This study aims to investigate the immunoregulatory mechanisms of lactoferrin formula and its four nutritional components (α-lactalbumin,N-acetylneuraminic acid,yeast β-glucan,and lactoferrin ) in RAW 264.7 macrophages.[Method ] M1 and M 2 macrophage polarization were induced by lipopolysaccharides (LPS) and interleukin (IL)-4,respectively.Quantitative real-time PCR,flow cytometry,and Western blot were employed to analyze macrophage polarization and related signaling pathways.[Result ] Lactoferrin formula enhanced the phagocytic activity of macrophages and dose-dependently inhibited M 1 polarization while promoting M 2 polarization within the range of 10~50 µg/mL.A combination of four nutritional components exhibited similar effects,with greater effects than the three-component combination.Additionally,the lactoferrin formula upregulated antigen-presenting molecules and antioxidant enzyme activity.It modulated macrophage polarization by inhibiting the mitogen-activated protein kinase (MAPK )/nuclear factor (NF)-κB pathway and activating the protein kinase B (Akt)/signal transducer and activator of transcription 6 (STAT 6) pathway,thereby alleviating inflammation.[Conclusion ] Lactoferrin formula exerts immunoregulatory effects by modulating macrophage polarization.

Publication Date

5-15-2025

First Page

146

Last Page

160

DOI

10.12441/spyswjs.20250225003

Recommended Citation

ZHOU, Mengying; ZHENG, Liujie; YAO, Ruiqing; WU, Hongyu; HU, Gangliang; HUO, Zhengquan; and NI, Yinhua (2025) "Nutritional Components and Immunoregulatory Mechanisms of Lactoferrin Formula," Journal of Food Science and Biotechnology: Vol. 44: Iss. 5, Article 16.
DOI: 10.12441/spyswjs.20250225003
Available at: https://spsw.spyswjs.cnjournals.com/journal/vol44/iss5/16

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