Preparation , Performance , and Application of a High-performance Affinity Chromatography Medium Based on Staphylococcus aureus Protein A

Authors

Zihao GUO, National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing , Jiangnan University , Wuxi 214122 , China
Chaoqing YAN, National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing , Jiangnan University , Wuxi 214122 , China
Haifeng XIA, National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing , Jiangnan University , Wuxi 214122 , ChinaFollow

Corresponding Author(s)

夏海锋（1979—），男，博士，副教授，硕士研究生导师，主要从事发酵工程、生化工程、生物分离工程以及学科交叉研究。E-mail：hfxia@jiangnan.edu.cn

Abstract

[Objective ] Staphylococcus aureus protein A (SPA ) is widely used for the purification of monoclonal antibodies due to its high specificity for immunoglobulin G (IgG).However,the high costs of commercial media have limited the further development of SPA.To address this issue,this paper aims to prepare a high-performance affinity chromatography medium based on SPA as the substitute of introduced affinity chromatography media.[Method ] SPA expressed by the recombinant strain was purified via Ni2+ affinity chromatography and then coupled to the Rigose HF matrix to prepare an self-prepared affinity chromatography medium.[Result ] The self-prepared medium had a static saturated adsorption capacity of 115.5 mg/mL (based on the volume of the medium ) and a dynamic adsorption capacity of 58.6 mg/mL (based on the volume of the medium ).When being used for the separation and purification of monoclonal antibody samples,the self-prepared affinity chromatography medium achieved the purity of over 95%,with a recovery rate of no less than 90%.The medium demonstrated comparable or superior alkali tolerance,reusability,and purification performance for monoclonal antibody samples to commercial media.[Conclusion ] A recombinant strain expressing SPA is successfully constructed,and the optimal preparation conditions of the self-prepared affinity chromatography medium are determined by optimization of the coupling conditions.The self-prepared affinity chromatography medium demonstrates research significance and commercial application value to a certain extent.

Publication Date

5-15-2025

First Page

137

Last Page

145

DOI

10.12441/spyswjs.20221220004

Recommended Citation

GUO, Zihao; YAN, Chaoqing; and XIA, Haifeng (2025) "Preparation , Performance , and Application of a High-performance Affinity Chromatography Medium Based on Staphylococcus aureus Protein A," Journal of Food Science and Biotechnology: Vol. 44: Iss. 5, Article 15.
DOI: 10.12441/spyswjs.20221220004
Available at: https://spsw.spyswjs.cnjournals.com/journal/vol44/iss5/15

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