Journal of Food Science and Biotechnology
Abstract
[Objective ] This study aimed to investigate the feasibility of the fermentation process for L-lysine production by immobilized Corynebacterium glutamicum.[Method ] Firstly,the effects of different volume fractions of seed medium on the fermentation stability of immobilized Corynebacterium glutamicum were examined.Subsequently,the impacts of immobilization carriers with different specifications on the fermentation performance were analyzed.Finally,a comparative analysis was conducted on the differences between the fermentation processes with free and immobilized Corynebacterium glutamicum.[Result ] The addition of 5% volume fraction of seed medium adequately sustained the growth and metabolic activities of Corynebacterium glutamicum.Higher volume fractions diverted carbon sources towards bacterial growth,thereby limiting product synthesis.The most favorable fermentation metrics were achieved with a specification of 0.061 g/cm2.Compared with free Corynebacterium glutamicum fermentation,immobilized Corynebacterium glutamicum exhibited a significantly shortened fermentation cycle and an increase of 16.24% in the acid production rate,which was attributable to the high cell density and protective effects of immobilization.Furthermore,the reduced biomass redirected more carbon sources towards product synthesis,resulting in higher conversion rates.[Conclusion ] The novel fermentation process employing immobilized Corynebacterium glutamicum demonstrates strong application feasibility,providing important insights for its future industrial implementation.
Publication Date
12-15-2025
First Page
62
Last Page
71
DOI
10.12441/spyswjs.20241206002
Recommended Citation
LIU, Qingguo; YING, Hanjie; and SUN, Wenjun
(2025)
"Fermentation Technology of L-Lysine Production by Immobilized Corynebacterium glutamicum,"
Journal of Food Science and Biotechnology: Vol. 44:
Iss.
12, Article 7.
DOI: 10.12441/spyswjs.20241206002
Available at:
https://spsw.spyswjs.cnjournals.com/journal/vol44/iss12/7
References
［1］ 李俊霖，郭传庄，王松江，等.赖氨酸的生物学功能及其在饲料中的应用 ［J］.中国饲料，2020（21）：63-66.LI J L，GUO C Z，WANG S J，et al.Research advance on biological activities and application of lysine ［J］.China Feed，2020（21）：63-66.（in Chinese ）
［2］ ALBREKTSEN S，KORTET R，SKOV P V，et al.Future feed resources in sustainable salmonid production：a review［J］.Reviews in Aquaculture，2022，14（4）：1790 -1812.
［3］ WENDISCH V F.Metabolic engineering advances and prospects for amino acid production ［J］.Metabolic Engineering，2020，58：17-34.
［4］ LIU J，XU J Z，RAO Z M，et al.Industrial production of L-lysine in Corynebacterium glutamicum：progress and prospects ［J］.Microbiological Research，2022，262：127101.
［5］ SCHILLING B，BASISTY N，CHRISTENSEN D G，et al.Global lysine acetylation in Escherichia coli results from growth conditions that favor acetate fermentation ［J］.Journal of Bacteriology，2019，201（9）：e00768 -18.
［6］ CHENG J，CHEN P，SONG A D，et al.Expanding lysine industry：industrial biomanufacturing of lysine and its derivatives ［J］.Journal of Industrial Microbiology and Biotechnology，2018，45（8）：719-734.
［7］ DO CARMO FÉLIX F K，LETTI L A J，DE MELO PEREIRA G V，et al.L-Lysine production improvement：a review of the state of the art and patent landscape focusing on strain development and fermentation technologies ［J］.Critical Reviews in Biotechnology，2019，39（8）：1031 -1055.
［8］ IKEDA M.Lysine fermentation：history and genome breeding［J］.Advances in Biochemical Engineering/Biotechnology，2017，159：73-102.
［9］ BECHER J，ZELDER O，HAFNER S，et al.From zero to hero design-based systems metabolic engineering of Corynebacterium glutamicum for L-lysine production ［J］.Metabolic Engineering，2011，13：159-168.
［10］ LIU J，OU Y，XU J Z，et al.L-lysine production by systems metabolic engineering of an NADPH auto-regulated Corynebacterium glutamicum ［J］.Bioresource Technology，2023，387：129701.
［11］ RAY D，ANAND U，JHA N K，et al.The soil bacterium，Corynebacterium glutamicum，from biosynthesis of value-added products to bioremediation：a master of many trades［J］.Environmental Research，2022，213：113622.
［12］ ZHAO Z Q，CAI M M，LIU Y R，et al.Genomics and transcriptomics-guided metabolic engineering Corynebacterium glutamicum for L-arginine production ［J］.Bioresource Technology，2022，364：128054.
［13］ 王金多，李澜潇，徐庆阳.L-赖氨酸全营养流加发酵工艺的研究 ［J］.中国调味品，2022，47（10）：93-97.WANG J D，LI L X，XU Q Y.Study on technology of total nutrient feeding fermentation of L-lysine［J］.China Condiment，2022，47（10）：93-97.（in Chinese ）
［14］ CHACÓN-NAVARRETE H，MARTÍN C，MORENO-GARCÍA J.Yeast immobilization systems for second-generation ethanol production：actual trends and future perspectives ［J］.Biofuels，Bioproducts and Biorefining，2021，15（5）：1549 -1565.
［15］ 张强，嵇冶.固定化细胞技术应用于酒精发酵中的研究进展［J］.化工进展，2017，36（4）：1404 -1409.ZHANG Q，JI Y.Research progress on cell immobilization technology in ethanol production ［J］.Chemical Industry and Engineering Progress，2017，36（4）：1404 -1409.（in Chinese ）
［16］ 刘芳，于建生.细胞固定化及其在酿酒工业中的应用研究进展［J］.酿酒科技，2013（8）：85-87.LIU F，YU J S.Research progress in cell immobilization and its application in liquor-making ［J］.Liquor-Making Science & Technology，2013（8）：85-87.（in Chinese ）
［17］ 周康熙，吴铮，冯哲瀚，等.凝胶珠固定化细胞发酵研究进展［J］.中国酿造，2021，40（3）：27-32.ZHOU K X，WU Z，FENG Z H，et al.Research progress in immobilized cell fermentation with gel beads ［J］.China Brewing，2021，40（3）：27-32.（in Chinese ）
［18］ 陈耕，倪育军，钟敏，等.包埋提高异常威克汉姆酵母醛脱氢酶的稳定性 ［J］.食品与机械，2022，38（5）：8-11，18.CHEN G，NI Y J，ZHONG M，et al.Preliminary study of encapsulation to improve stability of ALDH of Wickerhamomyces anomalus ［J］.Food & Machinery，2022，38（5）：8-11，18.（in Chinese ）
［19］ 段昌园，姜雪，曾宏威，等.表面偶极离子亲水磁珠固定化异柠檬酸裂解酶及其表征 ［J］.食品与生物技术学报，2024，43（1）：20-28.DUAN C Y，JIANG X，ZENG H W，et al.Immobilization of isocitrate lyase on amphoteric ion-rich hydrophilic magnetic beads and its characterization ［J］.Journal of Food Science and Biotechnology，2024，43（1）：20-28.（in Chinese ）
［20］ 张华伟，樊帅，杨兆勇，等.来源于 Anoxybacillus sp.SK3-4的普鲁兰酶 PulASK 固定化及酶学性质研究 ［J］.食品与生物技术学报，2023，42（4）：24-30.ZHANG H W，FAN S，YANG Z Y，et al.Immobilization of pullulanase PulASK from Anoxybacillus sp.SK3-4 and its enzymatic properties［J］.Journal of Food Science and Biotechnology，2023，42（4）：24-30.（in Chinese ）
［21］ LIU Q G，ZHAO N，ZOU Y N，et al.Feasibility of ethanol production from expired rice by surface immobilization technology in a new type of packed bed pilot reactor ［J］.Renewable Energy，2020，149：321-328.
［22］ PENG X W，ZHANG D，YUAN J Y，et al.Engineering bacterium for biofilm formation and L-lysine production in continuous fermentation ［J］.Bioresource Technology，2024，414：131567.
［23］ LIU Q G，CHENG H，WU J L，et al.Long-term production of fuel ethanol by immobilized yeast in repeated-batch simultaneous saccharification and fermentation of cassava ［J］.Energy & Fuels，2015，29（1）：185-190.
［24］ CHEN H Y，KHUMSUPAN D，PATEL A K，et al.Immobilization of Kluyveromyces marxianus K21 via coaxial electrospinning of PVA and sugarcane bagasse composite for bioethanol production ［J］.Applied Energy，2024，356：122405.
［25］ KUPRYASHINA M A，PONOMAREVA E G，PYLAEV T E.Immobilization of Azospirillum bacteria on various carriers［J］.Microbiology，2024，93（1）：104-111.
［26］ CADIZ J P R，AGCAOILI R P，MAMUAD R Y，et al.Fermentation of sweet sorghum （Sorghum bicolor L.Moench） using immobilized yeast （Saccharomyces cerevisiae ） entrapped in calcium alginate beads ［J］.Fermentation，2023，9（3）：272.
［27］ 高豪，陆家声，章文明，等.材料介导细胞固定化技术在生物发酵中的应用 ［J］.化工进展，2021，40（7）：3923 -3931.GAO H，LU J S，ZHANG W M，et al.Application of material-mediated cell immobilization technology in biological fermentation ［J］.Chemical Industry and Engineering Progress，2021，40（7）：3923 -3931.（in Chinese）
［28］ YANG Z Y.Immobilization of Enterococcus faecalis cells with chitosan：a new process for the industrial production of L-citrulline ［J］.Process Biochemistry，2015，50（7）：1056 -1060.
［29］ FENG S J，SRINIVASAN S，LIN Y H.Redox potential-driven repeated batch ethanol fermentation under very-high-gravity conditions ［J］.Process Biochemistry，2012，47（3）：523-527.