Goat Milk Formula Powder Ameliorates Lipopolysaccharide-induced Intestinal Inflammation

Authors

Yi SHAN, School of Food Engineering , Harbin University , Harbin 150086 , China
Xiaoling LIU, Heilongjiang Institute of Quality Supervision and Inspection , Harbin 150001 , China
Shumei WANG, School of Food Engineering , Harbin University , Harbin 150086 , ChinaFollow

Corresponding Author(s)

王淑梅（1979—），女，博士，教授，硕士研究生导师，主要从事食品微生物研究。E-mail：wangshumei24@126.com

Abstract

[Objective ] This study aimed to investigate the effects of goat milk formula powder on lipopolysaccharide (LPS)-induced intestinal inflammation and barrier impairment in mice.[Method ] Histopathological observation,real-time quantitative PCR,and enzyme-linked immunosorbent assay were used to assess changes in intestinal inflammation levels and barrier function-related indicators in mice following intervention with goat milk formula powder.Additionally,the composition of the gut microbiota was analyzed to elucidate its potential mechanisms of action.[Result ] Goat milk formula powder alleviated intestinal histopathology and increased the number of goblet cells.Compared with the model group,the goat milk formula powder intervention reduced the mass fractions of pro-inflammatory cytokines TNF- α,IL- 1β,and IL- 6 by 21.88%,28.30%,and 36.67%,respectively,while it increased the mass fraction of the anti-inflammatory cytokine IL-10 by 53.80%.Regarding intestinal permeability markers,it reduced the mass concentrations of D-lactate and enterotoxin by 16.14% and 32.52%,respectively,and decreased the activity of diamine oxidase (DAO ) by 8.74%.Meanwhile,it enhanced the mRNA expression levels of tight junction proteins ZO-1 and Claudin- 1 by 2.50- and 2.89-fold.Furthermore,the intervention increased the relative abundance of beneficial gut bacteria,including Akkermansia,Alistipes,Alloprevotella,and Muribaculum,and these microbial changes were correlated with the aforementioned inflammation and intestinal barrier indicators.[Conclusion ] Goat milk formula powder alleviated LPS-induced intestinal inflammation and enhanced barrier function in mice,with its mechanism of action potentially related to modulation of the gut microbiota structure.

Publication Date

12-15-2025

First Page

146

Last Page

155

DOI

10.12441/spyswjs.20241216001

Recommended Citation

SHAN, Yi; LIU, Xiaoling; and WANG, Shumei (2025) "Goat Milk Formula Powder Ameliorates Lipopolysaccharide-induced Intestinal Inflammation," Journal of Food Science and Biotechnology: Vol. 44: Iss. 12, Article 15.
DOI: 10.12441/spyswjs.20241216001
Available at: https://spsw.spyswjs.cnjournals.com/journal/vol44/iss12/15

References

［1］ 包云丽，汪哲，唐海茹，等.1990—2019年中国炎症性肠病疾病负担及变化趋势分析 ［J］.中国全科医学，2023，26（36）：4581 -4586.BAO Y L，WANG Z，TANG H R，et al.Disease burden and variation tendency of inflammatory bowel disease in China from 1990 to 2019［J］.Chinese General Practice，2023，26（36）：4581 -4586.（in Chinese ）
［2］ GUAN Q D.A comprehensive review and update on the pathogenesis of inflammatory bowel disease ［J］.Journal of Immunology Research，2019，2019（1）：7247238.
［3］ POPPE J，BOESMANS L，VIEIRA-SILVA S，et al.Differential contributions of the gut microbiota and metabolome to pathomechanisms in ulcerative colitis：an in vitro analysis［J］.Gut Microbes，2024，16（1）：2424913.
［4］ 李鸾，王丹丹，青格乐，等.植物乳植杆菌 IMAU 30043胞外多糖对脂多糖诱导的 RAW 264.7巨噬细胞氧化损伤的保护作用 ［J］.食品与生物技术学报，2024，43（9）：80-89.LI L，WANG D D，QING G L，et al.Protective of exopolysaccharide from Lactiplantibacillus plantarum IMAU 30043 against oxidative damage in RAW 264.7 macrophages ［J］.Journal of Food Science and Biotechnology，2024，43（9）：80-89.（in Chinese ）
［5］ 徐美余，罗成莹，辛维岗，等.复合益生菌对染菌白羽肉鸡生长性能及肠道形态、菌群的影响 ［J］.食品与生物技术学报，2024，43（2）：97-106.XU M Y，LUO C Y，XIN W G，et al.Effects of compound probiotics on growth performance，intestinal morphology and intestinal microbial composition of white feather broilers infected with Clostridium perfringens ［J］.Journal of Food Science and Biotechnology，2024，43（2）：97-106.（in Chinese ）
［6］ SOUSA Y R F，MEDEIROS L B，PINTADO M M E，et al.Goat milk oligosaccharides：composition，analytical methods and bioactive and nutritional properties ［J］.Trends in Food Science & Technology，2019，92：152-161.
［7］ VAN DER TOORN M V，CHATZIIOANNOU A C，PELLIS L，et al.Biological relevance of goat milk oligosaccharides to infant health ［J］.Journal of Agricultural and Food Chemistry，2023，71（38）：13935 -13949.
［8］ CHEN Q X，YIN Q L，XIE Q G，et al.Elucidating the physicochemical properties and surface composition of goat milk-based infant formula powders ［J］.Food Chemistry，2022，377：131936.
［9］ ZHANG K，ZHANG L N，ZHOU R Y，et al.Cow’s milk αS1-casein is more sensitizing than goat ’s milk αS1-casein in a mouse model ［J］.Food & Function，2022，13（12）：6484 -6497.
［10］ 张雷，王文利，程智美，等.乳铁蛋白生理活性及作用机理研究进展 ［J］.食品工业科技，2021，42（9）：388-395.ZHANG L，WANG W L，CHENG Z M，et al.Research progress on physiological activity and mechanism of lactoferrin ［J］.Science and Technology of Food Industry，2021，42（9）：388-395.（in Chinese ）
［11］ LE PARC A，DALLAS D C，DUAUT S，et al.Characterization of goat milk lactoferrin N-glycans and comparison with the N-glycomes of human and bovine milk［J］.Electrophoresis，2014，35（11）：1560 -1570.
［12］ LI X，GUI R，WANG X F，et al.Oligosaccharides isolated from Rehmannia glutinosa protect LPS-induced intestinal inflammation and barrier injury in mice ［J］.Frontiers in Nutrition，2023，10：1139006.
［13］ NING E J，SUN C W，WANG X F，et al.Artemisia argyi polysaccharide alleviates intestinal inflammation and intestinal flora dysbiosis in lipopolysaccharide-treated mice ［J］.Food & Med icine Homology，2024，1（1）：9420008.
［14］ 洪宝怡，吴桐，卢素云，等.幼儿配方羊奶粉对抗生素所致腹泻小鼠的影响 ［J］.中国乳品工业，2024，52（9）：14-21.HONG B Y，WU T，LU S Y，et al.Effect of infant sheep formula milk powder on antibiotic induced diarrhea in mice［J］.China Dairy Industry，2024，52（9）：14-21.（in Chinese）
［15］ 姜冰.肝硬化患者 DAO、内毒素、D-乳酸变化及在肠黏膜屏障功能评估中的作用 ［J］.中国实用医药，2017，12（33）：33-34.JIANG B.Changes in DAO，endotoxin，D-lactate acid and intestinal mucosa in patients with cirrhosis role in the assessment of barrier function ［J］.China Practical Medicine，2017，12（33）：33-34.（in Chinese ）
［16］ MARKOV A G.Claudins as tight junction proteins：the molecular element of paracellular transport ［J］.Rossiiskii Fiziologicheskii Zhurnal Imeni I.M.Sechenova，2013，99（2）：175-195.
［17］ CHEN Q X，CAO T，TANG H W，et al.Goat milk-based infant formula regulates intestinal barrier function and promotes the production of short-chain fatty acids ［J］.Food Science and Human Wellness，2024，13（6）：3150 -3158.
［18］ SEGATA N，IZARD J，WALDRON L，et al.Metagenomic biomarker discovery and explanation ［J］.Genome Biology，2011，12（6）：R60.
［19］ CANI P D，DEPOMMIER C，DERRIEN M，et al.Akkermansia muciniphila：paradigm for next-generation beneficial microorganisms ［J］.Nature Reviews Gastroenterology & Hepatology，2022，19（10）：625-637.
［20］ 杨夕缘，杨波，赵建新，等.动物双歧杆菌乳亚种对便秘的改善研究 ［J］.食品与生物技术学报，2024，43（7）：22-34.YANG X Y，YANG B，ZHAO J X，et al.Exploration of the Effect of Bifidobacterium animalis subsp.lactis on improving constipation ［J］.Journal of Food Science and Biotechnology，2024，43（7）：22-34.（in Chinese ）
［21］ RAU M，REHMAN A，DITTRICH M，et al.Fecal SCFAs and SCFA-producing bacteria in gut microbiome of human NAFLD as a putative link to systemic T-cell activation and advanced disease ［J］.United European Gastroenterology Journal，2018，6（10）：1496 -1507.
［22］ CHEN Q X，YIN Q L，XIE Q G，et al.Elucidating gut microbiota and metabolite patterns shaped by goat milk-based infant formula feeding in mice colonized by healthy infant feces ［J］.Food Chemistry，2023，410：135413.
［23］ HAN B，SHI L，BAO M Y，et al.Dietary ellagic acid therapy for CNS autoimmunity：targeting on Alloprevotella rava and propionate metabolism ［J］.Microbiome，2024，12（1）：114.
［24］ HUANG J M，LIU D，WANG Y W，et al.Ginseng polysaccharides alter the gut microbiota and kynurenine/tryptophan ratio，potentiating the antitumour effect of antiprogrammed cell death 1/programmed cell death ligand 1 （anti-PD- 1/PD-L 1） immunotherapy ［J］.Gut，2022，71（4）：734-745.