Comparison of the Bioactivities of Ziziphus jujuba Polysaccharide and Its Degradation Products

Authors

Chunling ZHOU, College of Bioscience and Engineering , Hebei University of Economics and Business , Shijiazhuang 050061 , China
Xiaoyan HUANG, College of Bioscience and Engineering , Hebei University of Economics and Business , Shijiazhuang 050061 , China
Xuan ZHENG, College of Bioscience and Engineering , Hebei University of Economics and Business , Shijiazhuang 050061 , China
Ju ZHANG, College of Bioscience and Engineering , Hebei University of Economics and Business , Shijiazhuang 050061 , China;Institute of Cash Crops , Hebei Academy of Agriculture and Forestry Sciences , Shijiazhuang 050041 , ChinaFollow

Corresponding Author(s)

张菊（1987—），女，博士，副教授，硕士研究生导师，主要从事食品营养与健康研究。E-mail：juzi437163804@163.com

Abstract

[Objective ] This study aims to compare Ziziphus jujuba polysaccharide (ZJP) and its degradation products in terms of their chemical composition and bioactivities.[Method ] ZJP was degraded by α-L-rhamnosidase,cellulase,snail enzyme,and trifluoroacetic acid to prepare different degradation products (ZJP-R,ZJP-C,ZJP-S,and ZJP-T ),and the chemical composition and degradation effects were compared.On the basis of in vitro antioxidant and probiotic activities,the in vivo antioxidant,anti-aging,anti-stress,and neuroprotective activities of ZJP and its degradation products were compared with Caenorhabditis elegans as a model system.[Result ] The mass fraction of reducing sugar and average degree of polymerization of ZJP showed significant variations among different degradation treatments.In addition,Sephadex G- 50 gel chromatography elution profile showed that ZJP-S had more oligosaccharide fragments with different relative molecular mass.In vitro assays demonstrated that degradation treatments significantly enhanced the free radical scavenging ability and total reducing power of ZJP and the growth activity of probiotics exposed to ZJP.In vivo assays in C.elegans revealed that the treatments with ZJP and its degradation products significantly reduced the reactive oxygen species (ROS ) accumulation,enhanced the superoxide dismutase (SOD ) activity,and decreased the malondialdehyde (MDA ) molality of C.elegans under oxidative stress.Furthermore,the treatments prolonged the lifespan,reduced the lipofuscin accumulation,and enhanced the resistance to heat stress and acute oxidative stress.ZJP demonstrated improved bioactivity after degradation,with ZJP-S exhibiting the most significant effects across multiple indicators.Additionally,both ZJP-S and ZJP-C showed significant neuroprotective activity in the C.elegans models of Huntington disease and Alzheimer ’s disease.[Conclusion ] Degradation treatment enhanced the bioactivity of ZJP.The greater bioactivity of ZJP-S might be associated with the more abundant oligosaccharides and reducing sugars from snail enzyme degradation.The findings provide a reference for the development and utilization of Z.jujuba resources.

Publication Date

11-15-2025

First Page

78

Last Page

89

DOI

10.12441/spyswjs.20240819002

Recommended Citation

ZHOU, Chunling; HUANG, Xiaoyan; ZHENG, Xuan; and ZHANG, Ju (2025) "Comparison of the Bioactivities of Ziziphus jujuba Polysaccharide and Its Degradation Products," Journal of Food Science and Biotechnology: Vol. 44: Iss. 11, Article 9.
DOI: 10.12441/spyswjs.20240819002
Available at: https://spsw.spyswjs.cnjournals.com/journal/vol44/iss11/9

References

［1］ 解玉军，李泽，崔小芳，等.酸枣化学成分及药理作用研究进展［J］.中成药，2021，43（5）：1269 -1275.XIE Y J，LI Z，CUI X F，et al.Research progress on chemical constituents and pharmacological effects of Ziziphus jujuba Mill.［J］.Chinese Traditional Patent Medicine，2021，43（5）：1269 -1275.（in Chinese ）
［2］ 段金廒，郭盛，严辉，等.药材生产过程副产物的价值发现和资源化利用是中药材产业扶贫的重要途径 ［J］.中国中药杂志，2020，45（2）：285-289.DUAN J A，GUO S，YAN H，et al.Value discovery and resource utilization of by-products in production process of medicinal materials are important ways for poverty alleviation with Chinese herbal medicine industry ［J］.China Journal of Chinese Materia Medica，2020，45（2）：285-289.（in Chinese ）
［3］ 杨冲，李宪松，刘孟军.酸枣的营养成分及开发利用研究进展［J］.北方园艺，2017（5）：184-188.YANG C，LI X S，LIU M J.Research progress on chemical constituents and utilization of sour jujube （Z.acidojujuba Cheng et Liu ） ［J］.Northern Horticulture，2017（5）：184-188.（in Chinese ）
［4］ JI X L，HOU C Y，YAN Y Z，et al.Comparison of structural characterization and antioxidant activity of polysaccharides from jujube （Ziziphus jujuba Mill.） fruit［J］.International Journal of Biological Macromolecules，2020，149：1008 -1018.
［5］ 孙延芳，梁宗锁，单长卷，等.野生酸枣果硒多糖纯化与光谱分析 ［J］.农业机械学报，2011，42（6）：148-151，147.SUN Y F，LIANG Z S，SHAN C J，et al.Purification and corresponding spectroscopic analysis of selenium polysaccharide in sour jujube fruits ［J］.Transactions of the Chinese Society for Agricultural Machinery，2011，42（6）：148-151，147.（in Chinese ）
［6］ YUE Y，WU S C，LI Z K，et al.Wild jujube polysaccharides protect against experimental inflammatory bowel disease by enabling enhanced intestinal barrier function［J］.Food & Function，2015，6（8）：2568 -2577.
［7］ 郎杰，崔娜，张立斌.酸枣果肉多糖保健功能的初步研究［J］.西北农林科技大学学报 （自然科学版 ），2014，42（2）：162-166.LANG J，CUI N，ZHANG L B.Preliminary study on healthcare effects of wild jujube pulp polysaccharides ［J］.Journal of Northwest A & F University （Natural Science Edition），2014，42（2）：162-166.（in Chinese ）
［8］ 朱玥明，陈朋，刘德川，等.海藻寡糖的生理活性与酶法转化研究进展 ［J］.食品与生物技术学报，2022，41（8）：22-34.ZHU Y M，CHEN P，LIU D C，et al.Progress on physiological activities and enzymatic conversion of algal oligosaccharides ［J］.Journal of Food Science and Biotechnology，2022，41（8）：22-34.（in Chinese ）
［9］ 刘昊，崔波，于滨.枸杞低聚糖体外抗氧化活性及增殖益生 菌 能 力 研 究 ［J］.食 品 与 机 械，2022，38（5）：138-142，148.LIU H，CUI B，YU B.Study on antioxidant activity and probiotic proliferation of Lycium barbarum oligosaccharides in vitro［J］.Food & Machinery，2022，38（5）：138-142，148.（in Chinese ）
［10］ 季珂，李恒，龚劲松，等.酶法制备壳寡糖及其抗肿瘤活性评价［J］.食品与生物技术学报，2021，40（6）：93-99.JI K，LI H，GONG J S，et al.Preparation of chitosan oligosaccharides by enzymatic hydrolysis and evaluation of antitumor activities ［J］.Journal of Food Science and Biotechnology，2021，40（6）：93-99.（in Chinese ）
［11］ 胡腾根.桑叶低聚糖的酶法制备及其通过肠道微生物介导调控糖脂代谢的作用机制研究 ［D］.广州：华南理工大学，2021.
［12］ 邓丽莉，潘晓倩，生吉萍，等.考马斯亮蓝法测定苹果组织微量可溶性蛋白含量的条件优化 ［J］.食品科学，2012，33（24）：185-189.DENG L L，PAN X Q，SHENG J P，et al.Optimization of experimental conditions for the determination of water soluble protein in apple pulp using coomassie brilliant blue method ［J］.Food Science，2012，33（24）：185-189.（in Chinese ）
［13］ 余菲，赵晓燕，周昌艳，等.不同品种生菜的多酚、黄酮差异比较与抗氧化活性 ［J］.食品科学，2024，45（13）：17-25.YU F，ZHAO X Y，ZHOU C Y，et al.Comparison of polyphenols，flavonoids and antioxidant activities of different varieties of lettuce ［J］.Food Science，2024，45（13）：17-25.（in Chinese ）
［14］ 刘宇，戴沅霖，马越，等.金银花粗多糖提取工艺优化及其抗氧化活性评价 ［J］.食品工业科技，2023，44（7）：188-196.LIU Y，DAI Y L，MA Y，et al.Extraction process optimization and antioxidant activity evaluation of crude polysaccharides from Lonicera japonica ［J］.Science and Technology of Food Industry，2023，44（7）：188-196.（in Chinese）
［15］ 吴雨烔，周礼红，方月月，等.小叶苦丁枝条提取物的植物乳杆菌发酵代谢组分析 ［J］.食品与生物技术学报，2023，42（6）：104-111.WU Y T，ZHOU L H，FANG Y Y，et al.Analysis of extracts of Ligustrum robustum （Rxob.） blume twigs fermented by Lactobacillus plantarum based on metabolomics ［J］.Journal of Food Science and Biotechnology，2023，42（6）：104-111.（in Chinese ）
［16］ 董婷，周志江，韩烨.乳酸片球菌 PA003发酵培养基及发酵条件的优化 ［J］.食品工业科技，2014，35（14）：192-196.DONG T，ZHOU Z J，HAN Y.Optimization of fermentation medium and fermentation conditions for Pediococcus acidilactici PA003［J］.Science and Technology of Food Industry，2014，35（14）：192-196.（in Chinese ）
［17］ LI W，LI Z Y，PENG M J，et al.Oenothein B boosts antioxidant capacity and supports metabolic pathways that regulate antioxidant defense in Caenorhabditis elegans［J］.Food & Function，2020，11（10）：9157 -9167.
［18］ WANG Q Q，ZHANG J，JIANG Y Y，et al.Caenorhabditis elegans as a model system for discovering bioactive compounds against polyglutamine-mediated neurotoxicity ［J］.Journal of Visualized Experiments，2021（175）：e63081.
［19］ ZHANG J，SHI R N，LI H F，et al.Antioxidant and neuroprotective effects of Dictyophora indusiata polysaccharide in Caenorhabditis elegans ［J］.Journal of Ethnopharmacology，2016，192：413-422.
［20］ LI N，LI Q C，HE X Y，et al.Antioxidant and anti-aging activities of Laminaria japonica polysaccharide in Caenorhabditis elegans based on metabonomic analysis［J］.International Journal of Biological Macromolecules，2022，221：346-354.
［21］ GHOSH T S，SHANAHAN F，O'TOOLE P W.The gut microbiome as a modulator of healthy ageing ［J］.Nature Reviews Gastroenterology & Hepatology，2022，19（9）：565-584.
［22］ HUI H P，XIN A Y，CUI H Y，et al.Anti-aging effects on Caenorhabditis elegans of a polysaccharide，O-acetyl glucomannan，from roots of Lilium davidii var.unicolor Cotton［J］.International Journal of Biological Macromolecules，2020，155：846-852.
［23］ PINCUS Z，MAZER T C，SLACK F J.Autofluorescence as a measure of senescence in C.elegans：look to red，not blue or green ［J］.Aging，2016，8（5）：889-898.
［24］ JEON H，SEOK C.Anti-aging properties of Ribes fasciculatum in Caenorhabditis elegans ［J］.Chinese Journal of Natural Medicines，2016，14（5）：335-342.
［25］ 剡宁宁.姜叶多酚提取及其对阿尔茨海默症的缓解活性研究［D］.雅安：四川农业大学，2022.
［26］ 李文青，祝孙婷，李圣洁，等.羊栖菜多糖及辅助超声降解对高脂斑马鱼的降血脂作用 ［J］.食品与生物技术学报，2024，43（5）：156-163.LI W Q，ZHU S T，LI S J，et al.Hypolipidemic effect of Sargassum fusiforme polysaccharide and ultrasonic-assisted degradation on hyperlipidemic zebrafish ［J］.Journal of Food Science and Biotechnology，2024，43（5）：156-163.（in Chinese ）
［27］ GUO D，YU K，SUN X Y，et al.Structural characterization and repair mechanism of gracilaria lemaneiformis sulfated polysaccharides of different molecular weights on damaged renal epithelial cells ［J］.Oxidative Medicine and Cellular Longevity，2018，2018：7410389.
［28］ 潘文锋.黄芪多糖降解物的制备及其抗衰老作用研究［D］.广州：广东药科大学，2020.