Journal of Food Science and Biotechnology
Abstract
[Objective ] This study aims to develop a rapid and accurate method for detecting epidermal growth factor (EGF ).[Method ] 4-Formylphenylboronic acid (4-FPBA ) was covalently anchored to the surface of aminocarbon nanotubes (NH 2-MWCNTs ) to synthesize nanocomposites (NH 2-MWCNTs@ 4-FPBA ),and NH 2-MWCNTs@ 4-FPBA was modified on a glassy carbon electrode.When EGF was present,4-FPBA captured the EGF template through benzaldehyde,and then the electrode was immersed in pyrrole solution to electropolymerize to form a pyrrole molecularly imprinted layer.Finally,after dissociation and removal of the EGF template in an acidic solution,a three-dimensional cavity complementary to the EGF template was formed.[Result ] The sensor had a wide linear range of 0.45~500.00 pg/mL and a low detection limit of 0.45 pg/mL,with excellent selectivity,reproducibility,and stability.[Conclusion ] The measurement using this molecularly imprinted electrochemical sensor showed that the mass concentration of EGF was 3.67 pg/mL in yoghurt and 29.03 pg/mL in ultra-high temperature transiently sterilized cow milk.
Publication Date
11-15-2025
First Page
59
Last Page
69
DOI
10.12441/spyswjs.20240721003
Recommended Citation
ZHANG, Ruyue; WANG, Yiwei; ZENG, Hongxiu; WANG, Bini; ZHANG, Fuxin; HUO, Zhenquan; and LIU, Yufang
(2025)
"Establishment of a Molecularly Imprinted Electrochemical Sensor NH 2-MWCNTs@ 4-FPBA for the Detection of Epidermal Growth Factor in Dairy Products,"
Journal of Food Science and Biotechnology: Vol. 44:
Iss.
11, Article 7.
DOI: 10.12441/spyswjs.20240721003
Available at:
https://spsw.spyswjs.cnjournals.com/journal/vol44/iss11/7
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