Antimicrobial Resistance and Transmission Risk of Foodborne Escherichia coli in Wet Markets

Authors

Sisi LI, School of Food Science and Biotechnology , Zhejiang Gongshang University , Hangzhou 310018 , China
Wei ZHOU, Zhejiang Center of Animal Disease Control , Hangzhou 311199 , China
Zhijin ZHOU, Zhejiang Center of Animal Disease Control , Hangzhou 311199 , China
Bin WANG, Zhejiang Center of Animal Disease Control , Hangzhou 311199 , China
Chengjun QIU, Zhejiang Center of Animal Disease Control , Hangzhou 311199 , China
Zhangsheng ZHANG, School of Food Science and Biotechnology , Zhejiang Gongshang University , Hangzhou 310018 , China
Qi ZHANG, School of Food Science and Biotechnology , Zhejiang Gongshang University , Hangzhou 310018 , China
Yun SUN, School of Food Science and Biotechnology , Zhejiang Gongshang University , Hangzhou 310018 , China
Xu XU, School of Food Science and Biotechnology , Zhejiang Gongshang University , Hangzhou 310018 , China
Tao SHEN, School of Food Science and Biotechnology , Zhejiang Gongshang University , Hangzhou 310018 , China
Jianzhong HAN, School of Food Science and Biotechnology , Zhejiang Gongshang University , Hangzhou 310018 , China
Daofeng QU, School of Food Science and Biotechnology , Zhejiang Gongshang University , Hangzhou 310018 , ChinaFollow

Corresponding Author(s)

曲道峰（1983—），男，博士，教授，博士研究生导师，主要从事食源性微生物快速检测及耐药菌环境适应性研究。E-mail：daofeng@mail.zjgsu.edu.cn

Abstract

[Objective ] To evaluate the antimicrobial resistance and transmission risk of foodborne Escherichia coli in wet markets,this study isolated E.coli from pork,aquatic products,vegetables,and wastewater collected from three wet markets in Hangzhou,Zhejiang.[Method ] The isolates were identified by 16S rRNA sequencing,and their antimicrobial resistance phenotypes were determined via the disk diffusion method.PCR was employed to detect antimicrobial resistance genes and integrons in the isolates.The antimicrobial resistance characteristics of E.coli were analyzed,and conjugation experiments were performed to assess the potential for horizontal transfer of antimicrobial resistance genes.Co-occurrence network analysis was conducted to evaluate the transmission risk of antimicrobial resistance among the isolates.[Result ] A total of 68 E.coli strains were isolated from 180 samples.The Kirby-Bauer (K-B) test revealed that most E.coli isolates were multidrug-resistant (MDR ).The resistance rates to amoxicillin,tetracycline,and sulfisoxazole exceeded 50%.PCR amplification revealed that the tetracycline resistance gene tetA had the highest detection rate,followed by sul1,blaTEM,and tetB.Moreover,E.coli isolates from the wet market B exhibited higher prevalence of antimicrobial resistance genes than those from wet markets A and C.The detection rate of the integrase gene intI1 was the highest at 30.88%,and eight intI1-positive strains carried gene cassettes.Conjugation experiments demonstrated that in three donor strains,tetA could be transferred via plasmid-mediated conjugation into the recipient strain EC600.Co-occurrence network analysis revealed a strong co-transmission relationship between antimicrobial resistance genes and mobile genetic elements among E.coli isolates.[Conclusion ] The author investigates the antimicrobial resistance profiles and dissemination risk of E.coli isolated from three wet markets in Hangzhou,Zhejiang,providing a theoretical basis for ensuring the food safety in wet markets.

Publication Date

11-15-2025

First Page

48

Last Page

58

DOI

10.12441/spyswjs.20240725003

Recommended Citation

LI, Sisi; ZHOU, Wei; ZHOU, Zhijin; WANG, Bin; QIU, Chengjun; ZHANG, Zhangsheng; ZHANG, Qi; SUN, Yun; XU, Xu; SHEN, Tao; HAN, Jianzhong; and QU, Daofeng (2025) "Antimicrobial Resistance and Transmission Risk of Foodborne Escherichia coli in Wet Markets," Journal of Food Science and Biotechnology: Vol. 44: Iss. 11, Article 6.
DOI: 10.12441/spyswjs.20240725003
Available at: https://spsw.spyswjs.cnjournals.com/journal/vol44/iss11/6

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