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Journal of Food Science and Biotechnology

Corresponding Author(s)

罗莲凤（1980—），女，硕士，正高级农艺师，主要从事茶叶精深加工、茶叶技术推广应用。E-mail：260564134@qq.com

Abstract

[Objective ] This study is intended to optimize the preparation process of Jinxuan black tea cream,enhance the content of tea polyphenols,and compare the effects of different processing methods on the volatile components of the tea cream.[Method ] With dried Jinxuan black tea as the raw material,response surface methodology was adopted to examine the impacts of tea-to-water ratio,enzyme dosage,ultrasonic power,and ultrasonic duration on the mass fraction of tea polyphenols in the black tea cream.Furthermore,headspace solid-phase microextraction (HS-SPME ) coupled with gas chromatography-mass spectrometry (GC-MS ) was employed to detect and analyze the volatile components in the black tea cream samples processed via three distinct methods:vacuum freeze concentration (NP),enzyme-assisted vacuum freeze concentration (WB),and rotary evaporation concentration combined with vacuum freeze concentration (B).[Result ] The optimal process conditions for the black tea cream were determined as follows:a tea-to-water ratio of 1 g∶20 mL,an enzyme dosage of 30 mg/g,ultrasonic power of 514 W,and ultrasonic duration of 21 min.Under these optimal conditions,the mass fraction of tea polyphenols in the Jinxuan black tea cream attained 269.34 mg/g.A total of 103,107,and 84 volatile components were identified from NP,WB,and B samples,respectively.Among them,volatile components with floral and fruity aromas,including linalool,benzyl alcohol,3-methylbutanal,benzaldehyde,phenylacetaldehyde,myrcene,and pyrrole,were detected in all the three samples (NP,WB,and B ).After enzymatic hydrolysis,the mass fraction of volatile components in the WB sample was 4 014.39 µg/kg higher than that in the NP sample.The volatile components with floral and fruity aromas,such as 3,7-dimethyl- 1,5,7-octatriene- 3-ol,limonene,and pyrazines,were not detected in sample B.[Conclusion ] Enzymatic hydrolysis combined with vacuum freeze-drying can effectively increase the mass fraction of volatile components in black tea cream.

Publication Date

11-15-2025

First Page

159

Last Page

172

DOI

10.12441/spyswjs.20240613001

References

［1］ 亓俊然，张龙雪，陈新颖，等.鲜叶萎凋前期低温处理对金萱白茶品质的影响 ［J］.食品工业科技，2022，43（18）：63-71.QI J R，ZHANG L X，CHEN X Y，et al.Effect of low temperature treatment on the quality of Jinxuan white tea in the early stage of fresh leaves withering ［J］.Science and Technology of Food Industry，2022，43（18）：63-71.（in Chinese）
［2］ 李赛君，雷雨，段继华，等.茶树品种金萱杂交亲和性和配合力研究 ［J］.茶叶通讯，2022，49（1）：18-24.LI S J，LEI Y，DUAN J H，et al.Study on cross compatibility and combining ability of Camellia sinensis cultivar Jinxuan ［J］.Journal of Tea Communication，2022，49（1）：18-24.（in Chinese ）
［3］ KRISETYADI B C，HERMANSYAH H.Effect of outlet temperature and total soluble solid in 2-stage spray dryer on black tea powder extract production ［J］.IOP Conference Series：Earth and Environmental Science，2021，782（3）：032087.
［4］ 谭超，刘华戎，龚加顺，等.不同加工工艺对红茶茶膏品质的影响 ［J］.茶叶科学，2013，33（4）：351-357.TAN C，LIU H R，GONG J S，et al.Effect of different processing technology on the quality of black tea paste ［J］.Journal of Tea Science，2013，33（4）：351-357.（in Chinese ）
［5］ 李官丽，吴秋月，陈锡霞，等.干燥方式对油茶粉品质及挥发性风味物质的影响 ［J］.食品与机械，2024，40（3）：210-216.LI G L，WU Q Y，CHEN X X，et al.Effects of drying methods on the quality and volatile flavor compounds of oil tea powder ［J］.Food & Machinery，2024，40（3）：210-216.（in Chinese ）
［6］ 陈佳星，赵姚姚，余丽娟，等.铁观音茶膏制备工艺及其抗氧化研究 ［J］.食品科技，2021，46（7）：112-115.CHEN J X，ZHAO Y Y，YU L J，et al.Preparation technology and antioxidant activity of Tieguanyin tea cream［J］.Food Science and Technology，2021，46（7）：112-115.（in Chinese ）
［7］ QU F F，ZHU X J，AI Z Y，et al.Effect of different drying methods on the sensory quality and chemical components of black tea ［J］.LWT-Food Science and Technology，2019，99：112-118.
［8］ 潘斐，刘通讯.酶在普洱茶膏加工工艺中的应用 ［J］.食品工业科技，2017，38（19）：79-83，95.PAN F，LIU T X.Application of enzymic treatment on the process of Pu-erh tea cream ［J］.Science and Technology of Food Industry，2017，38（19）：79-83，95.（in Chinese ）
［9］ 缪凤，王俊懿，朱海燕.外源纤维素酶对江华苦茶红茶品质的影响 ［J］.食品工业科技，2021，42（17）：38-46.MIAO F，WANG J Y，ZHU H Y.Effects of exogenous cellulase on the quality of black tea of JiangHua-KuCha ［J］.Science and Technology of Food Industry，2021，42（17）：38-46.（in Chinese ）
［10］ KAZEMI F，MASOUMI S Z，SHAYAN A，et al.Effect of green tea ointment on perineal pain and wound healing after episiotomy：a randomized double-blind clinical trial ［J］.European Journal of Integrative Medicine，2021，41：101258.
［11］ 杨霁虹，周汉琛，刘亚芹，等.基于 HS-SPME-GC-MS和OAV分析黄山地区不同茶树品种红茶香气的差异 ［J］.食品科学，2022，43（16）：235-241.YANG J H，ZHOU H C，LIU Y Q，et al.Differences in aroma components of black tea processed from different tea cultivars in Huangshan by using headspace-solid phase microextraction-gas chromatography-mass spectrometry and odor activity value ［J］.Food Science，2022，43（16）：235-241.（in Chinese ）
［12］ YU P H，HUANG Y J，LI Z Y，et al.Difference in aroma components of black teas processed on different dates in the spring season ［J］.Foods，2023，12（23）：4368.
［13］ HUANG W J，FANG S M，WANG J，et al.Sensomics analysis of the effect of the withering method on the aroma components of Keemun black tea ［J］.Food Chemistry，2022，395：133549.
［14］ HUANG J F，YAN T Y，YANG J F，et al.Aroma components analysis and origin differentiation of black tea based on ATD-GC-MS and E-nose ［J］.Horticulturae，2023，9（8）：885.
［15］ WU Q Y，ZHOU Z W，ZHANG Y N，et al.Identification of key components responsible for the aromatic quality of Jinmudan black tea by means of molecular sensory science ［J］.Foods，2023，12（9）：1794.
［16］ NIU Y W，MA Y W，XIAO Z B，et al.Characterization of the key aroma compounds of three kinds of Chinese representative black tea and elucidation of the perceptual interactions of methyl salicylate and floral odorants ［J］.Molecules，2022，27（5）：1631.
［17］ LIU C，WANG C，ZHENG T T，et al.Characterization of key odor-active compounds in Sun-dried black tea by sensory and instrumental-directed flavor analysis ［J］.Foods，2022，11（12）：1740.
［18］ LIU P P，ZHENG P C，GONG Z M，et al.Comparing characteristic aroma components of bead-shaped green teas from different regions using headspace solid-phase microextraction and gas chromatography-mass spectrometry/olfactometry combined with chemometrics ［J］.European Food Research and Technology，2020，246（9）：1703 -1714.
［19］ 王海斌，陈晓婷，孔祥海，等.速溶红茶茶多酚提取工艺优化及因子效应分析 ［J］.食品研究与开发，2017，38（13）：40-43.WANG H B，CHEN X T，KONG X H，et al.Extraction technology optimum of polyphenols in instant black tea and factor effect analysis ［J］.Food Research and Development，2017，38（13）：40-43.（in Chinese ）
［20］ 谢李玲，薛婉茹，李丹阳，等.外源酶对陈年武夷岩茶香气品质的改善作用 ［J］.食品研究与开发，2023，44（5）：155-164.XIE L L，XUE W R，LI D Y，et al.Exogenous enzymes improve the aroma quality of Wuyi rock tea ［J］.Food Research and Development，2023，44（5）：155-164.（in Chinese）
［21］ 马小雨，罗彩萍，刘悦.响应面优化微波辅助绿茶茶多酚提取工艺 ［J］.药学实践与服务，2023，41（2）：119-124，134.MA X Y，LUO C P，LIU Y.Optimization of microwave-assisted extraction of green tea polyphenols by response surface methodology ［J］.Journal of Pharmaceutical Practice and Service，2023，41（2）：119-124，134.（in Chinese）
［22］ 罗兰心，姜青，李翔，等.生物酶法提取铁观音茶梗中茶多酚工艺技术研究 ［J］.食品研究与开发，2020，41（24）：79-85.LUO L X，JIANG Q，LI X，et al.Study on technique of compound enzyme extraction of tea polyphenols from Tieguanyin tea stalks ［J］.Food Research and Development，2020，41（24）：79-85.（in Chinese ）
［23］ 端木传宇，孙洋，张瑜，等.黑莓多糖酶法提取工艺优化及体外降血糖活性研究 ［J］.食品工业科技，2024，45（22）：200-207.DUANMU C Y，SUN Y，ZHANG Y，et al.Optimization of enzymatic extraction process and in vitro hypoglycemic activity of blackberry polysaccharides ［J］.Science and Technology of Food Industry，2024，45（22）：200-207.（in Chinese ）
［24］ 李培睿，王丽娜.超声波辅助提取绿茶中茶多酚的工艺研究［J］.许昌学院学报，2021，40（5）：71-74.LI P R，WANG L N.Extraction process of TP from green tea assisted by ultrasonic wave ［J］.Journal of Xuchang University，2021，40（5）：71-74.（in Chinese ）
［25］ 徐如飞，金哲晨，许良，等.超声波提取安吉白茶茶多酚 工 艺 的 优 化 ［J］.贵 州 农 业 科 学，2020，48（6）：115-119.XU R F，JIN Z C，XU L，et al.Optimization of ultrasonic extraction technology for extracting polyphenols from Anji white tea ［J］.Guizhou Agricultural Sciences，2020，48（6）：115-119.（in Chinese ）
［26］ ZHANG S W，SUN L L，WEN S，et al.Analysis of aroma quality changes of large-leaf black tea in different storage years based on HS-SPME and GC-MS ［J］.Food Chemistry：X，2023，20：100991.
［27］ 杨沅思，坤吉瑞，代洪苇，等.晒青绿茶加工过程香气物质及其部分前体变化分析 ［J］.食品科学，2024，45（1）：133-142.YANG Y S，KUN J R，DAI H W，et al.Changes in aroma compounds and selected precursors of Sun-dried green tea during processing ［J］.Food Science，2024，45（1）：133-142.（in Chinese ）
［28］ ALASALVAR C，TOPAL B，SERPEN A，et al.Flavor characteristics of seven grades of black tea produced in Turkey［J］.Journal of Agricultural and Food Chemistry，2012，60（25）：6323 -6332.
［29］ 牛淼，李雄宇，杨洪焱，等.传统滇红工夫红茶与野生滇红工夫红茶香气特征分析 ［J］.食品工业科技，2024，45（23）：229-239.NIU M，LI X Y，YANG H Y，et al.Analysis of aroma characteristics of traditional and wild Dianhong Congou black tea［J］.Science and Technology of Food Industry，2024，45（23）：229-239.（in Chinese ）
［30］ WANG B Y，CHEN H M，QU F F，et al.Identification of aroma-active components in black teas produced by six Chinese tea cultivars in high-latitude region by GC-MS and GC-O analysis ［J］.European Food Research and Technology，2022，248（3）：647-657.
［31］ 梁轶琳，张灵枝，戴浩民，等.HS-SPME-GC-MS 分析武夷岩茶呈香挥发性物质 ［J］.食品与生物技术学报，2024，43（5）：110-121.LIANG Y L，ZHANG L Z，DAI H M，et al.Analysis of aroma volatile compounds in Wuyi rock tea based on HS-SPME-GC-MS ［J］.Journal of Food Science and Biotechnology，2024，43（5）：110-121.（in Chinese ）
［32］ 魏昊，蓝天梦，缪伊雯，等.基于感官组学分析不同足火方式对金牡丹工夫红茶香气的影响 ［J］.茶叶科学，2023，43（1）：109-123.WEI H，LAN T M，MIAO Y W，et al.Analysis of the effect of different full firing methods on the aroma of Jinmudan Congou black tea based on sensomics characterization ［J］.Journal of Tea Science，2023，43（1）：109-123.（in Chinese ）
［33］ MAO S H，LU C Q，LI M F，et al.Identification of key aromatic compounds in Congou black tea by partial least-square regression with variable importance of projection scores and gas chromatography-mass spectrometry/gas chromatography-olfactometry ［J］.Journal of the Science of Food and Agriculture，2018，98（14）：5278 -5286.
［34］ 艾安涛，陈曦，李燕丽，等.不同品种 “遵义红”红茶香气成分分析 ［J］.食品与生物技术学报，2023，42（4）：103-111.AI A T，CHEN X，LI Y L，et al.Analysis of aroma components in different varieties of “Zunyi red ” black tea［J］.Journal of Food Science and Biotechnology，2023，42（4）：103-111.（in Chinese ）
［35］ WANG J T，ZHU Y，SHI J，et al.Discrimination and identification of aroma profiles and characterized odorants in citrus blend black tea with different citrus species ［J］.Molecules，2020，25（18）：4208.
［36］ 周佳，苏丹，王志霞，等.基于 HS-SPME-GC-MS 和多元统计分析三种红茶特征挥发性成分 ［J］.食品工业科技，2023，44（17）：342-350.ZHOU J，SU D，WANG Z X，et al.Analysis of characteristic volatile components of three kinds of black tea based on HS-SPME-GC-MS and multivariate statistics ［J］.Science and Technology of Food Industry，2023，44（17）：342-350.（in Chinese ）
［37］ YE F，GUO X B，LI B，et al.Characterization of effects of different tea harvesting seasons on quality components，color and sensory quality of “Yinghong 9” and “Huangyu” large-leaf-variety black tea ［J］.Molecules，2022，27（24）：8720.
［38］ LIU H C，XU Y J，WU J J，et al.GC-IMS and olfactometry analysis on the tea aroma of Yingde black teas harvested in different seasons ［J］.Food Research International，2021，150：110784.
［39］ WANG J J，QU L C，YU Z M，et al.Targeted quantitative metabolomic and flavor objective quantification technique reveal the impact mechanism of shaking on black tea quality and non-volatile metabolites ［J］.Food Chemistry，2024，458：140226.

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