Effects of α-Amylase Addition on the Characteristics of Infant Rice Flour

Authors

Huihui LIU, Hunan Engnice of Nutrition Foods Co ., Ltd., Changsha 410000 , China
Zhiyong DAI, Hunan Engnice of Nutrition Foods Co ., Ltd., Changsha 410000 , ChinaFollow
Mengyi LI, Hunan Engnice of Nutrition Foods Co ., Ltd., Changsha 410000 , China
Jiao PENG, Hunan Engnice of Nutrition Foods Co ., Ltd., Changsha 410000 , China
Xing MENG, Hunan Engnice of Nutrition Foods Co ., Ltd., Changsha 410000 , China
Luyan LIAO, School of Food Science and Technology , Hunan Agricultural University , Changsha 410128 , China
Rongbin ZHANG, School of Food Science and Engineering , Central South University of Forestry and Technology , Changsha 410004 , China

Corresponding Author(s)

戴志勇（1978—），男，本科，中级工程师，主要从事婴幼儿食品加工研究。E-mail：daizhiyong@engnice.com

Abstract

[Objective ] This study aims to investigate the effects of α-amylase addition on the characteristics of infant rice flour.[Method ] Infant rice flour samples with different α-amylase addition were prepared.The basic composition,relative molecular mass of starch,gelatinization properties,hydration properties,texture properties,in vitro digestion characteristics,and microstructure of infant rice flour were determined.Furthermore,the correlations between the physicochemical characteristics and quality characteristics of infant rice flour were analyzed.[Result ] The weight-averaged molecular weight and the number-averaged molecular weight of starch in infant rice flour decreased with the increase in α-amylase addition.The gelatinization curve of infant rice flour with α-amylase addition was relatively gentle,and the gelatinization properties of infant rice flour without α-amylase addition were higher than those of infant rice flour supplemented with this enzyme (P<0.05).The water absorption index and swelling power decreased with the increase in α-amylase addition.Infant rice flour with α-amylase addition of 1.8 U/L had the highest sensory scores.The texture properties decreased with the increase in α-amylase addition.The infant rice flour with α-amylase addition showed small and numerous starch granules and denser pores on the surface.The mass fraction of ready digestible starch increased and that of resistant starch decreased in the infant rice flour with α-amylase addition.The sensory scores of infant rice flour were negatively correlated with the valley viscosity (P<0.01).The mass fraction of resistant starch,hardness,consistency,cohesion,and adhesion were positively correlated with the relative molecular mass of starch,water absorption index,and gelatinization properties (P<0.01),and they were negatively correlated with water solubility index (P<0.01).[Conclusion ] The characteristics of infant rice flour are significantly improved by α-amylase addition,and the comprehensive quality of infant rice flour with the addition of 1.8 U/L is the best.

Publication Date

11-15-2025

First Page

109

Last Page

118

DOI

10.12441/spyswjs.20240730001

Recommended Citation

LIU, Huihui; DAI, Zhiyong; LI, Mengyi; PENG, Jiao; MENG, Xing; LIAO, Luyan; and ZHANG, Rongbin (2025) "Effects of α-Amylase Addition on the Characteristics of Infant Rice Flour," Journal of Food Science and Biotechnology: Vol. 44: Iss. 11, Article 12.
DOI: 10.12441/spyswjs.20240730001
Available at: https://spsw.spyswjs.cnjournals.com/journal/vol44/iss11/12

References

［1］ 王俏，王骁音，Tino Landl，等.全球婴幼儿辅助食品发展状况与趋势 ［J］.中国妇幼健康研究，2021，32（12）：1836 -1842.WANG Q，WANG X Y，LANDL T，et al.The development status and trend of global complementary foods for infant and young children ［J］.Chinese Journal of Woman and Child Health Research，2021，32（12）：1836 -1842.（in Chinese ）
［2］ 刘昊，金忠兴，赵冰熔，等.酶制剂在婴儿营养米粉生产中的应用研究 ［J］.中国乳品工业，2005，33（4）：34-36.LIU H，JIN Z X，ZHAO R B，et al.Studies on amylase application in infant cereal production ［J］.China Dairy Industry，2005，33（4）：34-36.（in Chinese ）
［3］ 马文华，杨帆，钱晓洁，等.淀粉-脂质复合物耐消化性的研究进展：影响因素和形成机制 ［J］.食品工业科技，2025，46（9）：414-423.MA W H，YANG F，QIAN X J，et al.Research progress on the digestibility resistance of starch-lipid complexes：the influence factor and formation mechanism ［J］.Science and Technology of Food Industry，2025，46（9）：414-423.（in Chinese ）
［4］ 张云亮，王子妍，徐晨冉，等.婴幼儿米粉加工技术及品质评价的研究进展 ［J］.中国粮油学报，2021，36（2）：196-202.ZHANG Y L，WANG Z Y，XU C R，et al.Research progress on processing technology and quality evaluation of infant rice flour ［J］.Journal of the Chinese Cereals and Oils Association，2021，36（2）：196-202.（in Chinese ）
［5］ MINEKUS M，ALMINGER M，ALVITO P，et al.A standardised static in vitro digestion method suitable for food：an international consensus ［J］.Food & Function，2014，5（6）：1113 -1124.
［6］ 石红.谷基配方营养米粉酶法工艺关键技术及模拟消化分析［D］.杭州：浙江工商大学，2017.
［7］ 刘颖，王子妍，张煜，等.不同酶解程度对留胚米粉理化性质及消化性的影响研究 ［J］.中国食品添加剂，2024，35（1）：42-50.LIU Y，WANG Z Y，ZHANG Y，et al.Effects of enzymatic hydrolysis on physicochemical properties and digestibility of embryos retained rice flour ［J］.China Food Additives，2024，35（1）：42-50.（in Chinese ）
［8］ 胡居吾，吉雪，赵强，等.限制性酶解与喷雾干燥制备婴幼儿米粉及其体外消化性和流变性研究 ［J］.中国食品学报，2018，18（12）：118-128.HU J W，JI X，ZHAO Q，et al.In vitro digestibility and rheological properties of infant rice powder produced by limited hydrolysis and spray drying ［J］.Journal of Chinese Institute of Food Science and Technology，2018，18（12）：118-128.（in Chinese ）
［9］ SYAHARIZA Z A，LI E P，HASJIM J.Extraction and dissolution of starch from rice and sorghum grains for accurate structural analysis ［J］.Carbohydrate Polymers，2010，82（1）：14-20.
［10］ 郁映涛，肖刘洋，杨晓凡等.超声波结合酶解对玉米淀粉多层级结构及其吸附性能的影响 ［J］.食品科学，2024，45（5）：174-183.YU Y T，XIAO L Y，YANG X F，et al.Effect of ultrasonic treatment combined with enzymatic hydrolysis on the multilevel structure and adsorption properties of maize starch［J］.Food Science，2024，45（5）：174-183.（in Chinese ）
［11］ 刘惠惠.高膳食纤维挤压方便米饭的研制 ［D］.长沙：湖南农业大学，2022.
［12］ 刘芳宏，谭书明，邓泽祥，等.婴幼儿分段米粉辅料配方的研究［J］.食品工业，2018，39（4）：49-53.LIU F H，TAN S M，DENG Z X，et al.Study on auxiliary materials formula of subsection rice powder for the infant ［J］.The Food Industry，2018，39（4）：49-53.（in Chinese ）
［13］ 袁如英，韩飞，黄荣和.滚筒干燥结合酶法生产婴幼儿米粉的工艺研究 ［J］.现代食品，2018，24（11）：187-189.YUAN R Y，HAN F，HUANG R H.Study on the technology of producing infant rice powder by drum drying combined with enzymatic method ［J］.Modern Food，2018，24（11）：187-189.（in Chinese ）
［14］ 孙鹏伟，刘娅妮，李然，等.不同添加量的双孢蘑菇粉对酸奶流变学和质构特性的影响 ［J］.食品工业科技，2024，45（9）：54-61.SUN P W，LIU Y N，LI R，et al.Effects of different addition of Agaricus bisporus powders on rheological and textural properties of yogurt ［J］.Science and Technology of Food Industry，2024，45（9）：54-61.（in Chinese ）
［15］ DENG F，LI Q P，CHEN H，et al.Relationship between chalkiness and the structural and thermal properties of rice starch after shading during grain-filling stage ［J］.Carbohydrate Polymers，2021，252：117212.
［16］ 程冰，张乐乐，安艳霞，等.马铃薯抗性淀粉结构特征及体外消化特性的研究 ［J］.食品安全质量检测学报，2021，12（17）：6975 -6981.CHENG B，ZHANG L L，AN Y X，et al.Researched on structural characteristics and in vitro digestibility of potato resistant starch ［J］.Journal of Food Safety & Quality，2021，12（17）：6975 -6981.（in Chinese ）
［17］ ENGLYST H N，KINGMAN S M，CUMMINGS J H.Classification and measurement of nutritionally important starch fractions ［J］.European Journal of Clinical Nutrition，1992，46（Suppl 2）：S33-S50.
［18］ 瞿佳丽.加工过程中添加 α-淀粉酶和风味蛋白酶对红小豆沙食用品质的影响 ［D］.无锡：江南大学，2023.
［19］ LI M，LI J X，ZHU C H.Effect of ultrasound pretreatment on enzymolysis and physicochemical properties of corn starch ［J］.International Journal of Biological Macromolecules，2018，111：848-856.
［20］ KEERATIBURANA T，HANSEN A R，SOONTARANON S，et al.Porous high amylose rice starch modified by amyloglucosidase and maltogenic α-amylase ［J］.Carbohydrate Polymers，2020，230：115611.
［21］ 谭瑶瑶，吴亨，古碧，等.不同酶制备木薯抗性淀粉的性质比较［J］.食品工业科技，2013，34（11）：88-91.TAN Y Y，WU H，GU B，et al.Property comparison of cassava resistant starch prepared by different amylases［J］.Science and Technology of Food Industry，2013，34（11）：88-91.（in Chinese ）
［22］ CLAVER I P，ZHANG H H，LI Q，et al.Impact of the soak and the malt on the physicochemical properties of the sorghum starches ［J］.International Journal of Molecular Sciences，2010，11（8）：3002 -3015.
［23］ 戴晓慧，张名位，马永轩等.蒸汽酶解调质 -挤压膨化工艺改善速食米粉冲调分散性和预消化性 ［J］.现代食品科技，2020，36（10）：200-209，156.DAI X H，ZHANG M Wi，MA Y X，et al.Extrusion pretreated by steam conditioning accompanying starch enzymolysis improved the dispersibility and predigestion of instant rice flour ［J］.Modern Food Science and Technology，2020，36（10）：200-209，156.（in Chinese ）
［24］ BENAVENT-GIL Y，RODRIGO D，ROSELL C M.Thermal stabilization of probiotics by adsorption onto porous starches［J］.Carbohydrate Polymers，2018，197：558-564.［25］ 周显青，叶新悦，张玉荣，等.粳糯稻谷贮藏期间糊化特性的变化 ［J］.食品与发酵工业，2022，48（12）：60-67.ZHOU X Q，YE X Y，ZHANG Y R，et al.Study on the changes of gelatinization characteristics of japonica -glutinous rice during storage ［J］.Food and Fermentation Industries，2022，48（12）：60-67.（in Chinese ）
［26］ VAMADEVAN V，BERTOFT E.Impact of different structural types of amylopectin on retrogradation ［J］.Food Hydrocolloids，2018，80：88-96.
［27］ 阮蕴莹，邓媛元，张雁，等.不同葡萄糖当量值预消化大米膨化粉的理化性质和结构特性 ［J］.食品科学，2023，44（14）：29-36.RUAN Y Y，DENG Y Y，ZHANG Y，et al.Physicochemical and structural properties of pre-digested puffed rice flours with different dextrose equivalent values ［J］.Food Science，2023，44（14）：29-36.（in Chinese ）
［28］ 陈悦.酶解和发酵处理对杂粮代餐粉品质特性及微观结构影响的研究 ［D］.沈阳：沈阳师范大学，2022.
［29］ 袁海娜，尤玉如，储小军，等.生物技术应用于婴幼儿谷基配方米粉的工艺研究 ［J］.中国食品学报，2009，9（6）：121-127.YUAN H N，YOU Y R，CHU X J，et al.Studies on production technology of infant formula rice powder by biotechnology ［J］.Journal of Chinese Institute of Food Science and Technology，2009，9（6）：121-127.（in Chinese ）
［30］ 张冬媛，邓媛元，张名位，等.发芽-挤压-淀粉酶协同处理对速食糙米粉品质特性的影响 ［J］.中国农业科学，2015，48（4）：759-768.ZHANG D Y，DENG Y Y，ZHANG M W，et al.Effect of germination-extrusion-amylase assisted processing on quality properties of brown rice powder ［J］.Scientia Agricultura Sinica，2015，48（4）：759-768.（in Chinese ）
［31］ 马丽媛，狄冰倩，贺馨颖，等.复合酶酶解对谷物杂粮粉体 外 消 化 率 的 影 响 ［J］.食 品 工 业，2021，42（10）：123-127.MA L Y，DI B Q，HE X Y，et al.The effect of compound enzyme enzymolysis on the digestibility of cereal and multigrain flour in vitro［J］.The Food Industry，2021，42（10）：123-127.（in Chinese ）