Journal of Food Science and Biotechnology
Abstract
[Objective ] This study aims to investigate the effects and differences of the natural antioxidant (+)-catechin and its isomer (-)-epicatechin on the antioxidant activity of HEK 293T cells based on the nuclear factor erythroid 2-related factor 2 (Nrf2)-antioxidant response element (ARE ) pathway.[Method ] The effects of (+)-catechin and (-)-epicatechin on the viability of HEK 293T cells were investigated via the methyl thiazolyl tetrazolium (MTT ) assay.Reporter genes were employed to investigate the regulatory effects on the activation of ARE.The effects of (+)-catechin and (-)-epicatechin on the mRNA and proteins levels of the downstream factors [glutamate-cysteine ligase modifier subunit (GCLM ),NAD (P)H quinone dehydrogenase 1 (NQO 1),and heme oxygenase- 1 (HO- 1)] of the Nrf 2-ARE signaling pathway were explored by RT-PCR and Western blot,respectively.[Result ] The expression of reporter genes showed that ARE-mediated transcriptional activation was significantly induced by (+)-catechin and (-)-epicatechin at 20 μmol/L and 10 μmol/L,respectively.RT-PCR and Western blot results showed that (+)-catechin at 40 μmol/L significantly up-regulated the mRNA levels of GCLM,NQO 1,and HO- 1,and (-)-epicatechin at the same concentration significantly up-regulated the mRNA levels of NQO 1 and HO- 1.At 10 μmol/L,(+)-catechin up-regulated the protein levels of GCLM,NQO 1,and HO- 1,and (-)-epicatechin up-regulated the protein levels of GCLM and HO- 1.[Conclusion ] (+)-Catechin and (-)-epicatechin have different minimum effect concentrations on cells.Both of them have the ability to activate the Nrf 2-ARE signaling pathway,and the required concentration of (-)-epicatechin to activate the pathway is lower than that of (+)-catechin.In addition,the two compounds have different effects on the expression of genes and proteins downstream of the Nrf 2-ARE signaling pathway,with (+)-catechin outperforming (-)-epicatechin at the same concentration.
Publication Date
11-15-2025
First Page
100
Last Page
108
DOI
10.12441/spyswjs.20240909001
Recommended Citation
SUN, Chang; XIA, Ning; WANG, Chao; QIN, Fengxian; LYU, Chengyu; LIU, Jian; and LI, Tiezhu
(2025)
"Effects and Differences of (+)-Catechin and (-)-Epicatechin on Antioxidant Activity of HEK 293T Cells,"
Journal of Food Science and Biotechnology: Vol. 44:
Iss.
11, Article 11.
DOI: 10.12441/spyswjs.20240909001
Available at:
https://spsw.spyswjs.cnjournals.com/journal/vol44/iss11/11
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