Immunomodulatory Effects and Marker Components of Alcohol Extract from Acanthopanax senticosus (Rupr . et Maxim .) Harms- Ganoderma applanatum (Pers.) Pat.

Authors

Lihong WANG, School of Pharmacy , Jiamusi University , Jiamusi 154007 , ChinaFollow
Kairui SHENG, School of Pharmacy , Jiamusi University , Jiamusi 154007 , China
Ming LU, School of Pharmacy , Jiamusi University , Jiamusi 154007 , China
Jin SU, School of Pharmacy , Jiamusi University , Jiamusi 154007 , China
Yang PING, School of Pharmacy , Jiamusi University , Jiamusi 154007 , China
Yu ZHANG, School of Pharmacy , Jiamusi University , Jiamusi 154007 , China
Ji ZHAO, School of Pharmacy , Jiamusi University , Jiamusi 154007 , China
Yixia GONG, School of Pharmacy , Jiamusi University , Jiamusi 154007 , China

Corresponding Author(s)

王丽红（1968—），女，博士，教授，生药（中药）鉴定及品质评价和植物的开发利用。E-mail：wlh_6663@163.com

Abstract

[Objective ] The aim of this study was to analyze the compositional changes of the alcohol extracts of Acanthopanax senticosus (Rupr.et Maxim.) Harms (APS )-Ganoderma applanatum (Pers.) Pat.(GA) before and after compounding at a mass ratio of 5∶1 (mAPS∶mGA=5∶1) and to investigate the immunomodulatory effects and marker components of the extracts.[Method ] The mouse models of compromised immunity and delayed-type hypersensitivity (DTH ) were established with cytoxan (CTX ) and 2,4-dinitrochlorobenzene (DNCB ),respectively,to investigate the effects of the alcohol extracts on the immune system.Ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS ) was used to analyze the changes of the active ingredients before and after compounding.Differential thermal analysis was performed to determine whether new substances were generated after compounding.Single solvent extraction was employed to qualitatively analyze the chemical components.[Result ] The alcohol extract of A.senticosus -G.applanatum compounding at a mass ratio of 5∶1 possessed strong immunomodulatory effects.UHPLC-MS showed that the mass fractions of the active ingred ients in the alcohol extract were directly proportional to the mass of the two drugs.The differential thermal analysis showed that new compounds different from those in A.senticosus and G.applanatum were generated after the compounding of the two drugs,mainly including tannins,oxygen-containing heterocyclic anthraquinones,polysaccharides,volatile oils,and lipids.[Conclusion ] The strong immunomodulatory effects of the alcohol extract of A.senticosus -G.applanatum compounding at a mass ratio of 5∶1 are presumably related to the co-action of new compounds produced by the compounding,and these compounds could be used as maker components for the quality evaluation of A.senticosus -G.applanatum.

Publication Date

11-15-2025

First Page

90

Last Page

99

DOI

10.12441/spyswjs.20240521002

Recommended Citation

WANG, Lihong; SHENG, Kairui; LU, Ming; SU, Jin; PING, Yang; ZHANG, Yu; ZHAO, Ji; and GONG, Yixia (2025) "Immunomodulatory Effects and Marker Components of Alcohol Extract from Acanthopanax senticosus (Rupr . et Maxim .) Harms- Ganoderma applanatum (Pers.) Pat.," Journal of Food Science and Biotechnology: Vol. 44: Iss. 11, Article 10.
DOI: 10.12441/spyswjs.20240521002
Available at: https://spsw.spyswjs.cnjournals.com/journal/vol44/iss11/10

References

［1］ 彭颖.冠突散囊菌与茶多酚复合物对免疫抑制小鼠的保护作用［J］.食品与机械，2023，39（8）：160-165.PENG Y.Protective effect of Eurotium cristatum 　fermentation broth mixed with tea polyphenols on immunosuppressive mice ［J］.Food & Machinery，2023，39（8）：160-165.（in Chinese ）
［2］ 陈巍文，朱建津，黄建平，等.γ-谷维素和半胱胺对应激小鼠氧化还原状态及免疫功能的影响 ［J］.食品与生物技术学报，2022，41（4）：1-11.CHEN W W，ZHU J J，HUANG J P，et al.Effects of γ-oryzanol and cysteamine on redox status and immunity of stressed mice ［J］.Journal of Food Science and Biotechnology，2022，41（4）：1-11.（in Chinese ）
［3］ LI C G，LU Y，WANG J，et al.Immunoregulation of bovine lactoferrin together with osteopontin promotes immune system development and maturation ［J］.Food & Function，2024，15（2）：866-880.
［4］ SHANG L L，YAN Y G，LI Z，et al.Hydro-sensitive，in situ ultrafast physical self-gelatinizing，and red blood cells strengthened hemostatic adhesive powder with antibiosis and immunoregulation for wound repair ［J］.Advanced Science，2024，11（4）：2306528.
［5］ XU M Z，JI Y H.Immunoregulation of synovial macrophages for the treatment of osteoarthritis ［J］.Open Life Sciences，2023，18（1）：20220567.
［6］ 于纯淼，廖贤，于苗苗，等.刺五加在中国保健食品中的应用［J］.食品与机械，2023，39（8）：234-240.YU C M，LIAO X，YU M M，et al.Application of Acanthopanax senticosus in functional food in China ［J］.Food & Machinery，2023，39（8）：234-240.（in Chinese ）
［7］ HE Y，WANG Y M，ZHANG X，et al.Chemical characterization of small-molecule inhibitors of monoamine oxidase B synthesized from the Acanthopanax senticosus root with affinity ultrafiltration mass spectrometry ［J］.Rapid Communications in Mass Spectrometry，2020，34（8）：e8694.
［8］ HU H B，LI H M，HAN M H，et al.Chemical modification and antioxidant activity of the polysaccharide from Acanthopanax leucorrhizus ［J］.Carbohydrate Researc h，2020，487：107890.
［9］ LAU K M，YUE G G，CHAN Y Y，et al.A review on the immunomodulatory activity of Acanthopanax senticosus and its active components ［J］.Chinese Medicine，2019，14：25.
［10］ LI T，FERNS K，YAN Z Q，et al.Acanthopanax senticosus：photochemistry and anticancer potential ［J］.The American Journal of Chinese Medicine，2016，44（8）：1543 -1558.
［11］ 徐锦堂.中国药用真菌学 ［M］.北京：北京医科大学、中国协和医科大学联合出版社，1997：805.
［12］ 国家中医药管理局 《中华本草 》编委会.中华本草：1卷［M］.上海：上海科学技术出版社，1999：533-534.
［13］ 朱有昌.东北药用植物 ［M］.哈尔滨：黑龙江科学技术出版社，1989：1240.［14］ 赵继鼎.中国灵芝新编 ［M］.北京：科学出版社，1989：11-35.
［15］ LIU X Y，LIU S L，WEI H W，et al.Preliminary species diversity and community phylogenetics of wood-inhabiting basidiomycetous fungi in the Dabie Mountains，Central China reveal unexpected richness ［J］.IMA Fungus，2023，14（1）：23.
［16］ 梁耕源.FIP-gap 基因影响树舌灵芝菌丝体生长及转录组分析［D］.沈阳：沈阳农业大学，2023.
［17］ 魏婷，何先元，朱潇宏，等.九制黄精及其配伍对 CUMS抑郁模型小鼠物质能量代谢的影响 ［J］.食品与机械，2022，38（7）：180-185，207.WEI T，HE X Y，ZHU X H，et al.Comparative study of processed Polygonatum cyrtonema and its compatibility affecting on material and energy metabolism in CUMS depressed model mice ［J］.Food & Machinery，2022，38（7）：180-185，207.（in Chinese ）
［18］ GAO Y，WU X X，ZHAO N，et al.Scientific connotation of the compatibility of traditional Chinese medicine from the perspective of the intestinal flora ［J］.Frontiers in Pharmacology，2023，14：1152858.
［19］ 王志昊，周文静，费程浩，等.中药复方及组分配伍优化策略与实践 ［J］.中国实验方剂学杂志，2025，31（1）：299-310.WANG Z H，ZHOU W J，FEI C H，et al.Optimization strategy and practice of traditional Chinese medicine compound and its component compatibility ［J］.Chinese Journal of Experimental Traditional Medical Formulae，2025，31（1）：299-310.（in Chinese ）
［20］ 李晶，魏健.刺五加、枸杞子配伍对糖尿病模型小鼠降血糖作用的研究 ［J］.食品研究与开发，2017，38（9）：179-182.LI J，WEI J.Effect of compatibility of Acanthopanaxs enticosus and Chinese wolfberry fruit on reducing blood sugar in diabetic model mice ［J］.Food Research and Development，2017，38（9）：179-182.（in Chinese ）
［21］ 张翠.黄芪和刺五加配伍组方增强鸡免疫功能的实验研究［D］.长春：吉林大学，2013.
［22］ 郎佳佳.基于免疫调节和氧化应激探究益经汤治疗卵巢储备功能减退的疗效机制 ［D］.济南：山东中医药大学，2023.
［23］ 卢铭，王凤芝，李琪，等.不同配伍比例刺五加和树舌的镇静催眠作用 ［J］.安徽农业科学，2019，47（3）：150-153.LU M，WANG F Z，LI Q，et al.Sedative and hypnotic effects of different compatibility ratios of Acanthopanax and tree tongue ［J］.Journal of Anhui Agricultural Sciences，2019，47（3）：150-153.（in Chinese ）
［24］ 杨青春，庞怡，陈绍红，等.浒苔多糖 EP2对小鼠免疫功能的影响 ［J］.中国海洋药物，2018，37（3）：41-47.YANG Q C，PANG Y，CHEN S H，et al.Effects of EP 2 fraction of Enteromorpha polysaccharidson immune function in mice ［J］.Chinese Journal of Marine Drugs，2018，37（3）：41-47.（in Chinese ）
［25］ 张娜，杨晓丹，刘树民.刺五加多糖对 2、4-二硝基氟苯（DNFB）诱发的超敏反应作用及初步机制研究 ［J］.中医药信息，2015，32（3）：26-28.ZHANG N，YANG X D，LIU S M.Effect of ASP on hypersensitivity induced by DNFB and its mechanism ［J］.Information on Traditional Chinese Medicine，2015，32（3）：26-28.（in Chinese ）
［26］ 张弓，黄剑林，高鹏.绿萝花总多糖对小鼠免疫功能的增强作用 ［J］.中成药，2018，40（8）：1839 -1841.
［27］ ISHIDA M，SASAKI T，NISHI K，et al.Suppressive effect of ethanol extract from mango （Mangifera indica L.） peel on IgE production in vitro and in vivo［J］.Bioscience，Biotechnology，and Biochemistry，2018，82（4）：732-739.（in Chinese ）
［28］ LIU S，WANG K J，LIN S Z，et al.Comparison of the effects between tannins extracted from different natural plants on growth performance，antioxidant capacity，immunity，and intestinal flora of broiler chickens ［J］.Antioxidants，2023，12（2）：441.
［29］ XIN D D，LI H H，ZHOU S Y，et al.Effects of anthraquinones on immune responses and inflammatory diseases［J］.Molecules，2022，27（12）：3831.
［30］ YANG F J，YANG L，WANG W J，et al.Enrichment and purification of syringin，eleutheroside E and isofraxidin from Acanthopanax senticosus by macroporous re sin［J］.International Journal of Molecular Sciences，2012，13（7）：8970 -8986.
［31］ 国家药典委员会编委会.中国药典 2015版第一部 ［M］.北京：化学工业出版社，2015：206.
［32］ LI W F，XING W，WANG S C，et al.An online coupled peritoneal macrophage/cell membrane chromatography and high-performance liquid chromatography/mass spectrometry method to screen for anti-inflammatory components from the Chinese traditional medicine Chloranthus multistachys Pei［J］.Biomedical Chromatography，2013，27（11）：1580 -1586.
［33］ KABATA H，MORO K，KOYASU S.The group 2 innate lymphoid cell （ILC2） regulatory network and its underlying mechanisms.Immunological Reviews，2018，286（1）：37-52.
［34］ SU X Q，LIU B，GONG F T，et al.Isofraxidin attenuates IL- 1β-induced inflammatory response in human nucleus pulposus cells ［J］.Journal of Cellular Biochemistry，2019，120（8）：13302 -13309.
［35］ KHANAL P，PATIL B M，CHAND J，et al.Anthraquinone derivatives as an immune booster and their therapeutic option against COVID- 19［J］.Natural Products and Bioprospecting，2020，10（5）：325-335.
［36］ YING Y，HAO W.Immunomodulatory function and anti-tumor mechanism of natural polysaccharides：a review［J］.Frontiers in Immunology，2023，14：1147641.
［37］ YANG S B，SHAN C L，MA X，et al.Immunomodulatory effect of Acanthopanax senticosus polysaccharide on immunosuppressed chickens ［J］.Poultry Science，2021，100（2）：623-630.
［38］ 林碧霞.金线莲挥发油成分的提取及体外免疫活性研究［D］.福州：福建医科大学，2017.
［39］ SU G Q，WANG L，ZHOU X W，et al.Effects of essential oil on growth performance，digestibility，immunity，and intestinal health in broilers ［J］.Poultry Science，2021，100（8）：101242.