Advances in the Discovery of Active Natural Products by Large Fragment Cloning

Authors

Dandan ZENG, School of Health Science and Engineering , University of Shanghai for Science and Technology , Shanghai 200093 , China
Jiayu CHEN, State Key Laboratory of Bioreactor Engineering , East China University of Science and Technology , Shanghai 200237 , China
Tianlu MO, School of Health Science and Engineering , University of Shanghai for Science and Technology , Shanghai 200093 , ChinaFollow

Corresponding Author(s)

莫天录（1988—），男，博士，副教授，硕士研究生导师，主要从事活性天然产物发现和生物活性研究。E-mail：mo-tianlu@usst.edu.cn

Abstract

Active natural products have diverse structures and activities and can be a major source of small molecule drugs.Given the emergence of multi-drug resistant bacteria and superbacteria,there is an urgent need to develop new active natural products.Large fragment cloning has been an important tool for the discovery of active natural products in recent years,and it has demonstrated obvious advantages in obtaining the biosynthetic gene clusters of active natural products.This approach,combined with efficient cluster-targeted screening methods,has significantly accelerated the discovery of active natural products.This review summarizes a wide variety of active natural products.Furthermore,this paper elaborates on the principles and applications of several large fragment cloning technologies,including artificial chromosome technology,Red/ET recombination system,and TAR technology coupled with the CRISPR-Cas 9 system.This paper compares the advantages,disadvantages,and application scopes of different technologies and envisions their future prospects,aiming to provide theoretical guidance for the research and development of active natural products.

Publication Date

11-15-2025

First Page

1

Last Page

12

DOI

10.12441/spyswjs.20240328003

Recommended Citation

ZENG, Dandan; CHEN, Jiayu; and MO, Tianlu (2025) "Advances in the Discovery of Active Natural Products by Large Fragment Cloning," Journal of Food Science and Biotechnology: Vol. 44: Iss. 11, Article 1.
DOI: 10.12441/spyswjs.20240328003
Available at: https://spsw.spyswjs.cnjournals.com/journal/vol44/iss11/1

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