Enhanced Expression of PEPC Promotes the Synthesis of 4-Hydroxyisoleucine by Corynebacterium glutamicum

Authors

Chuhan HU, Key Laboratory of Industrial Biotechnology , Ministry of Education , Jiangnan University , Wuxi 214122 , China
Feng SHI, Key Laboratory of Industrial Biotechnology , Ministry of Education , Jiangnan University , Wuxi 214122 , ChinaFollow

Corresponding Author(s)

史锋（1970—），女，博士，教授，硕士研究生导师，主要从事微生物代谢、合成生物学研究。E-mail：shifeng@jiangnan.edu.cn

Abstract

[Objective ] This study aims to achieve the efficient biosynthesis of 4-hydroxyisoleucine (4-HIL ) in Corynebacterium glutamicum (C.glutamicum ).[Method ] The isoleucine (Ile) inducible promoters (PbrnFEN) of varying strengths were used to dynamically upregulate the expression of the isoleucine dioxygenase (IDO ) gene ido and the Vitreoscilla hemoglobin (VHb ) gene vgb.On this basis,constitutive promoters of different strengths were employed to enhance the expression of the phosphoenolpyruvate carboxylase (PEPC ) gene ppc and the pyruvate carboxylase gene pyc.[Result ] Among the recombinant strains harboring different P brnFEN promoters,strain C.glutamicum SN-I 7V7 (SN-I 7V7) was selected.After 144 h of fermentation with this strain,the concentration of 4-HIL reached (109.14±1.03) mmol/L,representing a 46.69% increase compared with the control strain C.glutamicum SN 02 (SN02).Subsequently,overexpression of ppc with the strong promoter P tacM led to the development of strain C.glutamicum RS-MP (RS-MP ),an elite 4-HIL producer.This strain achieved a 4-HIL concentration of (145.76±6.04) mmol/L,a 33.55% improvement over the control strain SN-I 7V7.[Conclusion ] The screened P brnFEN combinations and the ppc overexpression strategy provide a theoretical basis for the efficient biosynthesis of 4-HIL in C.glutamicum.

Publication Date

9-15-2025

First Page

163

Last Page

172

DOI

10.12441/spyswjs.20240315003

Recommended Citation

HU, Chuhan and SHI, Feng (2025) "Enhanced Expression of PEPC Promotes the Synthesis of 4-Hydroxyisoleucine by Corynebacterium glutamicum," Journal of Food Science and Biotechnology: Vol. 44: Iss. 9, Article 16.
DOI: 10.12441/spyswjs.20240315003
Available at: https://spsw.spyswjs.cnjournals.com/journal/vol44/iss9/16

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