Journal of Food Science and Biotechnology
Abstract
Photodynamic antimicrobial technology (PDT ),as a non-thermal physical bactericidal method,has received increasing attention due to its high efficiency,low residue,and lack of resistance induction.Natural pigments,as food-grade photosensitizers exhibit excellent optical responsiveness and biocompatibility.Studies have shown that curcumin,chlorophylls,and porphyrin-based pigments exhibit favorable photodynamic antimicrobial efficacy in various food matrices including fruits,vegetables,meat,and drinking water.The author summarizes the photodynamic antimicrobial mechanisms of representative natural pigments,and analyzes the effects of light parameters,photosensitizer concentrations,and food matrices on photodynamic antimicrobial efficacy.Furthermore,the recent progress of PDT applications in fruit and vegetable surface disinfection,drinking water sterilization,and smart packaging is reviewed,with a focus on the role of nanocarriers and composite films in enhancing photodynamic antimicrobial performance.Finally,the industrial application potential of natural pigment-mediated PDT in food processing is discussed,along with the challenges regarding photosensitizer stability and process standardization.Future research directions are proposed,emphasizing synergistic antimicrobial mechanisms and the development of functional materials for practical implementation.
Publication Date
9-15-2025
First Page
1
Last Page
11
DOI
10.12441/spyswjs.20250401003
Recommended Citation
ZHAO, Lei; JIANG, Wenli; SHEN, Qingshan; KE, Jingxuan; MA, Yanli; and ZHANG, Lifang
(2025)
"Natural Pigment-mediated Photodynamic Antimicrobial Technology : Mechanisms and Food Applications,"
Journal of Food Science and Biotechnology: Vol. 44:
Iss.
9, Article 1.
DOI: 10.12441/spyswjs.20250401003
Available at:
https://spsw.spyswjs.cnjournals.com/journal/vol44/iss9/1
References
[1] SARHAN O M.Tackling biofilm resistance of gram-positive and gram-negative bacteria against levofloxacin via nanotechnology and essential oils [J].Journal of Pharmaceutical Innovation,2024,20(1):5.
[2] HE J,ZHANG S,HU Z,et al.Antibacterial photodynamic inactivation of pH-responsive microspheres based on chitosan/carboxymethyl chitosan with hypericin and its application in food preservation [J].Food Bioscience,2025,71106992 -106992.
[3] 潘海军,王锋,马路凯,等.姜黄素光动力技术在微生物杀菌中的研究进展 [J].保鲜与加工,2025,25(1):130-138.PAN H J,WANG F,MA L K,et al.Progress in curcumin-mediated photodynamic technology for microbial sterilization[J].Storage and Process,2025,25(1):130-138.(in Chinese )
[4] KIM M J,HWA T,SUK B,et al.Antibacterial effect of 405±5 nm light emitting diode illumination against Escherichia coli O157:H7,Listeria monocytogenes,and Salmonella on the surface of fresh-cut mango and its influence on fruit quality [J].International Journal of Food Microbiology,2017,244:82-89.
[5] 王百川.低温预制食品中单核细胞增生李斯特菌生长模型的构建及光动力抑菌的应用 [D].江西:南昌大学,2022.
[6] SRIMAGAL A,RAMESH T,SAHU J K.Effect of light emitting diode treatment on inactivation of Escherichia coli in milk[J].LWT-Food Science and Technology,2016,71:378-385.
[7] DOS SANTOS R F,CAMPOS B S,DE ASSIS REGO FILHO F,et al.Photodynamic inactivation of S.aureus with a water-soluble curcumin salt and an application to cheese decontamination [J].Photochemical & Photobiological Sciences,2019,18(11):2707 -2716.
[8] 檀茜倩,麻冰玉,王丹,等.光动力技术及其在生鲜食品保鲜中的应用研究进展 [J].食品与生物技术学报,2022,41(7):100-110.TAN X Q,MA B Y,WANG D,et al.Research progress of photodynamic technology and its application in fresh food preservation [J].Journal of Food Science and Biotechnology,2022,41(7):100-110.(in Chinese )
[9] ZHANG Y,YAN H J,TANG J W,et al.Dual-mode antibacterial core-shell gold nanorod@mesoporous-silica/curcumin nano complexes for efficient photothermal and photodynamic therapy [J].Journal of Photochemistry and Photobiology A:Chemistry,2022,425:113722.
[10] LV Y B,LI P Y,CEN L,et al.Gelatin/carboxymethylcellulose composite film combined with photodynamic antibacterial:new prospect for fruit preservation [J].International Journal of Biological Macromolecules,2024,257:128643.
[11] 赵南昕.pH响应型智能介孔二氧化硅载体应用于金黄色葡萄球菌生物膜的光动力杀菌研究 [D].无锡:江南大学,2022.
[12] CHEN H L,TANG C H.Metal polyphenol network-modified chitin nanocrystals reinforce versatile biomass films:active packaging,intelligent visualization and controlled photothermal sterilization [J].Chemical Engineering Journal,2024,500:157114.
[13] 杨宇恒,宋子伟,郑宇航,等.负载姜黄素 -氧化锌纳米颗粒的水凝胶膜的制备及其对冷鲜鸡肉的保鲜效果[J].食品工业科技,2025,46(19):327-335.YANG Y H,SONG Z W,ZHENG Y H,et al.Preparation of hydrogel films loaded with curcumin-zinc oxide nanoparticles and its preservation effect on low-temperature chilled chicken meat [J].Science and Technology of Food Industry,2025,46(19):327-335.(in Chinese )
[14] 王小情,刘忠义,余元善,等.叶绿素镁钠盐对液态食品中Staphylococcus aureus 的光动力杀菌研究 [J].现代食品科技,2013,29(3):463-466,478.WANG X Q,LIU Z Y,YU Y S,et al.Photodynamic sterilization of Staphylococcus aureus in liquid food by Na-chlorophyllin [J].Modern Food Science and Technology,2013,29(3):463-466,478.(in Chinese )
[15] ZHANG Y C,DING Z Y,SHAO C B,et al.Chlorophyllin-based 405 nm light photodynamic improved fresh-cut pakchoi quality at postharvest and inhibited the formation of biofilm [J].Foods,2022,11(16):2541.
[16] 舒丽枝,时苗苗,张牧焓,等.卟啉类化合物和游离铁对鸡胸肉肌原纤维蛋白理化特性的影响 [J].江苏农业学报,2024,40(10):1952 -1961.SHU L Z,SHI M M,ZHANG M H,et al.Effect of porphyrins and free iron on physicochemical properties of myofibrillar protein of chicken breast muscle [J].Jiangsu Journal of Agricultural Sciences,2024,40(10):1952 -1961.(in Chinese )
[17] PALACIOS Y B,SIMONETTI S O,CHAVEZ C H,et al.“Illuminated glycoporphyrins ”:a photodynamic approach for Candida albicans inactivation [J].Journal of Photochemistry and Photobiology B:Biology,2025,264:113105.
[18] LAN M H,ZHAO S J,LIU W M,et al.Photosensitizers for photodynamic therapy [J].Advanced Healthcare Materials,2019,8(13):1900132.
[19] 郑双芝,王晓迪,张蕾,等.光动力杀菌技术及其杀灭食源性有害微生物的研究进展 [J].食品与发酵工业,2023,49(21):321-328.ZHENG S Z,WANG X D,ZHANG L,et al.Photodynamic anti-microbial technology and its research progress in killing foodborne harmful microorganisms [J].Food and Fermentation Industries,2023,49(21):321-328.(in Chinese )
[20] LU N,WANG Z G,ZHANG X,et al.Effects of curcumin-based photodynamic method on protein degradation of oysters [J].International Journal of Food Science & Technology,2021,56(8):4050 -4061.
[21] ZHANG Z,LONG S R,CAO J F,et al.Revealing the photodynamic stress in situ with a dual-mode two-photon 1O2 fluorescent probe [J].ACS Sensors,2020,5(5):1411 -1418.
[22] ZHOU Z L,CHEN R X,LI P Z,et al.Natural borneol improves cellular uptake of curcumin to enhance its photodynamic bactericidal activity against Escherichia coli ATCC 8739[J].Food Microbiology,2025,127:104686.
[23] ZHANG J W,ZHENG P Y,LI J,et al.Curcumin-mediated sono-photodynamic treatment inactivates Listeria monocytogenes via ROS-induced physical disruption and oxidative damage [J].Foods,2022,11(6):808.
[24] HUANG J M,CHEN B W,LI H H,et al.Enhanced antibacterial and antibiofilm functions of the curcumin-mediated photodynamic inactivation against Listeria monocytogenes [J].Food Control,2020,108:106886.
[25] ZHANG Z Q,QIN J R,WANG Z,et al.Sodium copper chlorophyll mediated photodynamic treatment inactivates Escherichia coli via oxidative damage [J].Food Research International,2022,157:111472.
[26] TAN L J,ZHAO Y,LI Y F,et al.Potent eradication of mixed-species biofilms using photodynamic inactivation coupled with slightly alkaline electrolyzed water [J].LWT-Food Science and Technology,2022,155:112958.
[27] OLIVEIRA H,ARAÚJO P,PEREIRA A R,et al.Photoactivated cell-killing amino-based flavylium compounds[J].Scientific Reports,2021,11:22005.
[28] TEERAKAPONG A,DAMRONGRUNGRUANG T,SATTAYUT S,et al.Efficacy of erythrosine and cyanidin- 3-glucoside mediated photodynamic therapy on Porphyromonas gingivalis biofilms using green light laser[J].Photodiagnosis and Photodynamic Therapy,2017,20:154-158.
[29] PING Y Y,ZHANG L,WANG W Z,et al.Visible light-driven multichannel concerted antibacterial films with photodynamically enhanced self-cascading chemodynamic actions for long-term preservation of perishable food [J].Food Chemistry,2025,471:142787.
[30] RIVAS AIELLO M B,GHILINI F,MARTÍNEZ PORCEL J E,et al.Riboflavin-mediated photooxidation of gold nanoparticles and its effect on the inactivation of bacteria[J].Langmuir,2020,36(28):8272 -8281.
[31] SONG L L,ZHANG F,YU J S,et al.Antifungal effect and possible mechanism of curcumin mediated photodynamic technology against Penicillium expansum [J].Postharvest Biology and Technology,2020,167:111234.
[32] YANG W,WANG Z Y,LI Q,et al.Photodynamic inactivation using natural bioactive compound prevents and disrupts the biofilm produced by Staphylococcus saprophyticus [J].Molecules,2021,26(16):4713.
[33] 于金珅,张芳.姜黄素介导的光动力技术对鲜切马铃薯的杀菌效果 [J].食品工业科技,2021,42(4):259-263,270.YU J S,ZHANG F.Effects of curcumin-mediated photodynamic technology on bactericidal efficacy of fresh-cut potatoes [J].Science and Technology of Food Industry,2021,42(4):259-263,270.(in Chinese )
[34] 何荣强,高土玲,符天晓,等.光动力杀菌技术在鲜切水果保鲜中的研究进展 [J].保鲜与加工,2025,25(2):150-156.HE R Q,GAO T L,FU T X,et al.Research progress of photodynamic antimicrobial technology in fresh-cut fruits preservation [J].Storage and Process,2025,25(2):150-156.(in Chinese )
[35] LIU Y,LI Y J,SHAO C,et al.Curcumin-based residue-free and reusable photodynamic inactivation system for liquid foods and its application in freshly squeezed orange juice[J].Food Chemistry,2024,458:140316.
[36] 闫元元.叶绿素镁钠盐介导的光动力技术对金黄色葡萄球菌的灭活及其生物被膜的清除作用研究 [D].上海:上海海洋大学,2021.
[37] 王倩,薛舟,王涛,等.姜黄素在食品包装中的应用进展[J].现代食品科技,2024,40(7):313-323.WANG Q,XUE Z,WANG T,et al.Application of curcumin in food packaging [J].Modern Food Science & Technology,2024,40(7):313-323.(in Chinese )
[38] 张力,蒋建勤.卟啉类光敏剂的研究进展 [J].云南化工,2018,45(11):1-6.ZHANG L,JIANG J Q.Research progress of porphyrin photosensitizers [J].Yunnan Chemical Technology,2018,45(11):1-6.(in Chinese )
[39] MAISCH T,SPANNBERGER F,REGENSBURGER J,et al.Fast and effective:intense pulse light photodynamic inactivation of bacteria [J].Journal of Industrial Microbiology & Biotechnology,2012,39(7):1013 -1021.
[40] KREITNER M,WAGNER K H,ALTH G,et al.Haematoporphyrin- and sodium chlorophyllin-induced phototoxicity towards bacteria and yeasts:a new approach for safe foods [J].Food Control,2001,12(8):529-533.
[41] 施源,李璟,胡嘉淼,等.基于 Citespace 的光动力杀菌技术研究进展可视化分析 [J].食品工业科技,2024,45(10):335-352.SHI Y,LI J,HU J M,et al.Bibliometric analysis of the research progressing in photodynamic antimicrobial technology based on Citespace [J].Science and Technology of Food Industry,2024,45(10):335-352.(in Chinese)
[42] 黄璐瑶,杜艳瑜,陆晓丹,等.大黄鱼鱼卵磷脂乳化姜黄素工艺的研究 [J].食品与发酵工业,2020,46(13):196-202.HUANG L Y,DU Y Y,LU X D,et al.Study on the technology of emulsifying curcumin in phospholipids oflarge yellow croaker roe [J].Food and Fermentation Industries,2020,46(13):196-202.(in Chinese )
[43] 赵若晴.蔬菜汁中金黄色葡萄球菌生长预测模型的构建及光动力杀菌技术研究 [D].长春:吉林大学,2020.
[44] ZHENG S Z,YAN H,DENG P W,et al.Enhancement of curcumin-mediated photodynamic inactivation against Pseudomonas fluorescens on fish fillets combined with ε-polylysine hydrochloride [J].LWT-Food Science and Technology,2025,217:117344.
[45] GUFFEY J S,PAYNE W C,MOTTS S D,et al.Inactivation of Salmonella on tainted foods:using blue light to disinfect cucumbers and processed meat products[J].Food Science & Nutrition,2016,4(6):878-887.
[46] ZHANG X,LI X,JIANG Y.Interaction between chlorophyll derivatives and proteins in dairy products under light exposure [J].Journal of Dairy Science,2021,104(5):5123 -5132.
[47] HYUN J E,LEE S Y.Antibacterial effect and mechanisms of action of 460~470 nm light-emitting diode against Listeria monocytogenes and Pseudomonas fluorescens on the surface of packaged sliced cheese [J].Food Microbiology,2020,86:103314.
[48] CHAI Z Y,ZHANG F,LIU B J,et al.Antibacterial mechanism and preservation effect of curcumin-based photodynamic extends the shelf life of fresh-cut pears [J].LWT-Food Science and Technology,2021,142:110941.
[49] DE OLIVEIRA E F,TOSATI J V,TIKEKAR R V,et al.Antimicrobial activity of curcumin in combination with light against Escherichia coli O157:H7 and Listeria innocua:applications for fresh produce sanitation [J].Postharvest Biology and Technology,2018,137:86-94.
[50] GAO J W,MATTHEWS K R.Effects of the photosensitizer curcumin in inactivating foodborne pathogens on chicken skin [J].Food Control,2020,109:106959.
[51] LUO C S,XIE F,CHEN Q Q,et al.Starch-nanoencapsulated polyphenol-induced polysaccharide gel coatings with efficient preservation capability [J].Food Chemistry,2025,467:142203.
[52] KINGSLEY D H,PEREZ-PEREZ R E,BOYD G,et al.Evaluation of 405 nm monochromatic light for inactivation of Tulane virus on blueberry surfaces [J].Journal of Applied Microbiology,2018,124(4):1017 -1022.
[53] CORRÊA T Q,BLANCO K C,GARCIA É B,et al.Effects of ultraviolet light and curcumin-mediated photodynamic inactivation on microbiological food safety:a study in meat and fruit [J].Photodiagnosis and Photodynamic Therapy,2020,30:101678.
[54] 王洋,赵瑜玲,陈孟涵,等.姜黄素联合乙二胺四乙酸对荧光假单胞菌的光动力灭活作用 [J].食品科学,2022,43(21):8-15.WANG Y,ZHAO Y L,CHEN M H,et al.Photodynamic inactivation effect of curcumin combined with ethylene diamine tetraacetic acid on Pseudomonas fluorescens [J].Food Science,2022,43(21):8-15.(in Chinese )
[55] 曾巧辉,余杏同,区灿盛,等.姜黄素光动力杀灭即食鸡肉中沙门氏菌的研究 [J].佛山科学技术学院学报 (自然科学版 ),2022,40(1):19-25.ZENG Q H,YU X T,OU C S,et al.Study on curcumin-mediated photodynamic inactivation of Salmonella on ready-to-eat chicken [J].Journal of Foshan University (Natural Science Edition ),2022,40(1):19-25.(in Chinese )
[56] 庄迪.姜黄素-虫胶纳米颗粒光敏抑菌明胶膜的制备及保鲜机制 [D].杨凌:西北农林科技大学,2024.
[57] 林以琳,李世洋,赖丹宁,等.姜黄素介导光动力减菌技术 对 缢 蛏 的 保 鲜 效 果 [J].农 业 工 程 学 报,2020,36(16):320-326.LIN Y L,LI S Y,LAI D N,et al.Effects of curcumin-mediated of anti-microbial photodynamic technology on preservation of razor clam [J].Transactions of the Chinese Society of Agricultural Engineering,2020,36(16):320-326.(in Chinese )
[58] JIAO L,LI Y W,TIAN M Y,et al.Novel halogenated curcumin-mediated photodynamic inactivation for the preservation of small yellow croaker (Larimichthys polyactis ) [J].Journal of Agricultural and Food Chemistry,2024,72(33):18720 -18730.
[59] LI H H,TAN L J,CHEN B W,et al.Antibacterial potency of riboflavin-mediated photodynamic inactivation against Salmonella and its influences on tuna quality [J].LWT-Food Science and Technology,2021,146:111462.
[60] LI Q Y,WANG B C,PENG S Y,et al.Photodynamic inactivation of Listeria monocytogenes using a natural aggregation-induced emission photosensitizer and its application in salmon preservation [J].LWT-Food Science and Technology,2024,193:115762.
[61] GONG C,LI Y J,GAO R C,et al.Inactivation of specific spoilage organism (Pseudomonas ) of sturgeon by curcumin-mediated photodynamic inactivation [J].Photodiagnosis and Photodynamic Therapy,2020,31:101827.
[62] KUZNETSOV A N A,YUZHAKOV A O A,STRAKHOVS KAY AM G,et al.New heterogeneous photosensitizers with phthalocyanine molecules covalently linked to aminopropyl silica gel [J].Journal of Porphyrins and Phthalocyanines,2011,15(7n08):718-726.
[63] BONNETT R,KRYSTEVA M A,LALOV I G,et al.Water disinfection using photosensitizers immobilized on chitosan[J].Water Research,2006,40(6):1269 -1275.
[64] GOMES A L R,RIBEIRINHO-SOARES S,MADEIRA L M,et al.Disinfection of secondary urban wastewater using hydrogen peroxide combined with UV/visible radiation:effect of operating conditions and assessment of microorganism competition [J].Water,2025,17(4):596.
[65] 邹林红.青稞麸皮 -花青素 /碳量子点智能双层膜的制备、理化性能及鱼肉保鲜的应用 [D].雅安:四川农业大学,2024.
[66] ZHANG J J,ZHANG J N,ZHANG R J,et al.Preparation of photodynamic-controlled release packaging for pork preservation and its visualization [J].Food Chemistry,2025,473:143005.
[67] 张俊俊.猪肉感控保鲜一体化的智能包装膜研究 [D].镇江:江苏大学,2023.
[68] ABDOU E S,GALHOUM G F,MOHAMED E N.Curcumin loaded nanoemulsions/pectin coatings for refrigerated chicken fillets [J].Food Hydrocolloids,2018,83:445-453.
[69] LUO L P,SU W Q,HASSAN M F,et al.Photothermal functionalized antibacterial packaging film with controllable release capability for fruit preservation [J].Food Research International,2025,206:116079.
[70] NING H Y,LU L X,ZHANG Y M,et al.Development of novel sodium alginate-based light-responsive controlled-release active packaging film [J].International Journal of Biological Macromolecules,2025,304:140780.
[71] NI Y S,SHI S,LI M,et al.Visible light responsive,self-activated bionanocomposite films with sustained antimicrobial activity for food packaging [J].Food Chemistry,2021,362:130201.
[72] 沈永康,朱峰,冯雪锋,等.壳聚糖基保鲜膜 /涂层的制备及应用 [J].包装工程,2025,46(1):79-88.SHEN Y K,ZHU F,FENG X F,et al.Preparation and application of chitosan-based fresh-keeping films/coatings[J].Packaging Engineering,2025,46(1):79-88.(in Chinese)
[73] WANG F,WANG R H,PAN Y J,et al.Gelatin/chitosan films incorporated with curcumin based on photodynamic inactivation technology for antibacterial food packaging[J].Polymers,2022,14(8):1600.
[74] WANG D F,WANG X,ZHOU S S,et al.Radish residue carbon dots-based novel starch/chitosan film with high antioxidant,biocompatibility,and antibacterial activities for salmon fillets ’ active packaging [J].International Journal of Biological Macromolecules,2024,273:133107.
[75] 张磊.载姜黄素脂质包覆介孔二氧化硅纳米颗粒的制备及性能评价 [D].天津:河北工业大学,2019.
[76] 黄荣.基于界面交联的负载姜黄素乳液体系的构建及其保护效果研究 [D].武汉:华中农业大学,2024.
[77] 曹洪玉.氮杂卟啉和杂原子卟啉电子吸收光谱理论研究[D].大连:大连理工大学,2019.
[78] MEDAGLIA S,OTRI I,BERNARDOS A,et al.Synergistic antimicrobial photodynamic therapy using gated mesoporous silica nanoparticles containing curcumin and polymyxin B [J].International Journal of Pharmaceutics,2024,654:123947.[79] CONTRERAS J,ALCÁZAR-VALLE M,LUGO-CERVANTES E,et al.Color degradation kinetics and antioxidant potential of black bean anthocyanin-rich extracts in commercial beverages [J].Current Developments in Nutrition,2024,8:102671.
[80] SOLEIMANI F,KARIMI A R,HADIZADEH M.Multifunctional riboflavin/chitosan-based dianhydride crosslinked hydrogels:photodynamic therapy,antioxidant,and antibacterial properties [J].Next Research,2025,2(1):100139.
[81] MCCAUGHAN B,ROUANET C,FOWLEY C,et al.Enhanced ROS production and cell death through combined photo- and sono-activation of conventional photosensitising drugs [J].Bioorganic & Medicinal Chemistry Letters,2011,21(19):5750 -5752.
[82] 王德华.CUR-SPDT 对哈维氏弧菌的灭活效果及在虾滑冷藏保鲜中的应用研究 [D].福州:福建农林大学,2022.
[83] BAEK K H,KIM H J,KANG T,et al.Blue light promotes bactericidal action of plasma-activated water against Staphylococcus aureus on stainless steel surfaces[J].Innovative Food Science & Emerging Technologies,2021,69:102663.
[84] 唐雅兰,蒋学金.纳米技术在食品包装的应用研究 [J].中国包装,2024,44(11):37-41.TANG Y L,JIANG X J.Study on the application of nanotechnology in food packaging [J].China Packaging,2024,44(11):37-41.(in Chinese )
[85] NI Y S,LI Y M,WANG M Y,et al.Chitosan-based packaging films with antibacterial-sterilization integrated continuous activity for extending the shelf life of perishable foods [J].International Journal of Biological Macromolecules,2024,275:133351.
[86] ZHU Y B,JIANG T,MAO Z Y,et al.Synergistic bactericidal effects of photodynamic inactivation technology combined with citric acid on Staphylococcus aureus [J].LWT-Food Science and Technology,2025,216:117366
[87] 刘帅言.共载花青素和姜黄素的多荷环糊精自组装递送体系的构建及应用研究 [D].长春:吉林大学,2024.