Journal of Food Science and Biotechnology
Abstract
[Objective ] The research on the synergistic use of lactic acid bacteria and yeast in sourdough is beneficial for meeting the industrial production demand of fermentation agents for baking food.[Method ] This study investigated the acid tolerance,acid production,enzyme production,gas production,and alcohol production abilities of various strains during the fermentation process by measuring the microbial count,pH,stretching area,elongation,hardness,viscosity,storage modulus (G′),loss modulus (G″),etc.of bread dough.The effects of different fer mentation agents on the total titratable acidity (TTA ),stretching characteristics,texture characteristics,and rheological properties of b read dough were investigated.[Result ] Lacticaseibacillus paracasei LG0260,Lactiplantibacillus plantarum LG1034,Lactococcus lactis LG0827,as well as Saccharomyces cerevisiae J2808 and S.cerevisiae J2815,maintained good acid production rates and abilities throughout the fermentation process.Especially,L.paracasei LG0260,Kluyveromyces marxianus J2828,and L.lactis LG0827 exhibited the highest phytase enzyme activity (23.21 mU/mL ),amylase enzyme activity (0.82 U/mL ),and protease enzyme activity (2.51 U/mL ),respectively.In addition,the fermentation agent MY 2L1 prepared by co-culture fermentation of S.cerevisiae J2808 and L.paracasei LG0260 effectively improved the stretching area and elongation of bread dough,with the highest values of 242 cm2 and 181 mm,respectively.[Conclusion ] Liquid strains have greater impacts on the hardness and viscosity of sourdough than commercial Angel yeast.The mixed fermentation with liquid strains enhances the fluid properties of the dough.This study can provide technical reference for mining type Ⅱ sourdough fermentation agents and improving sourdough quality.
Publication Date
8-15-2025
First Page
69
Last Page
78
DOI
10.12441/spyswjs.20250115002
Recommended Citation
CHEN, Yumeng; WANG, Weijun; SHEN, Shuling; LI, Yanhua; MA, Jingjing; HUANG, Menghan; and LIU, Yuhang
(2025)
"Screening of Excellent Lactic Acid Bacteria and Yeast Fermentation Agents and Effects of Mixed Fermentation on the Quality of Bread Dough,"
Journal of Food Science and Biotechnology: Vol. 44:
Iss.
8, Article 8.
DOI: 10.12441/spyswjs.20250115002
Available at:
https://spsw.spyswjs.cnjournals.com/journal/vol44/iss8/8
References
［1］ GOBBETTI M，DE ANGELIS M，DI CAGNO R，et al.Novel insights on the functional/nutritional features of the sourdough fermentation ［J］.International Journal of Food Microbiology，2018，302：103-113.
［2］ ISPIRLI H，DEMIRBAS F，YÜZER M O，et al.Identification of lactic acid bacteria from spontaneous rye sourdough and determination of their functional characteristics［J］.Food Biotechnology，2018，32：222-235.
［3］ LIANG J J，CHAI K M，WANG X Y，et al.From gluten structure to immunogenicity：investigating the effects of lactic acid bacteria and yeast co-fermentation on wheat allergenicity in steamed buns ［J］.Food Chemistry，2025，492（1）：145356.
［4］ 张国华，张纬珍，梁武龙，等.一株高产胞外多糖乳酸菌的鉴定及其特性研究 ［J］.中国食品学报，2020，20（12）：229-238.ZHANG G H，ZHANG W Z，LIANG W L，et al.Studies on identification and characterization of a high-yield exopolysaccharides lactic acid bacteria ［J］.Journal of Chinese Institute of Food Science and Technology，2020，20（12）：229-238.（in Chinese ）
［5］ LIU L，WU J L，YANG J T，et al.Isolation and fermentation characteristics of γ-Aminobutyric acid-producing lactic acid bacteria from Yujiangsuan，a traditional miao ethnic fermented condiment ［J］.Food Science，2021，42（18）：73-79.
［6］ XIANG L P，FAN B Q，YANG Z L，et al.Screening and identification of a strain of Saccharomyces cerevisiae and its characteristics ［J］.Food and Machinery，2021，37（06）：51-55.
［7］ ADOM K K，SORRELLS M E，LIU R H.Phytochemical profiles and antioxidant activity of wheat varieties ［J］.Journal of Agricultural and Food Chemistry，2003，51（26）：7825 -7834.
［8］ 邢君梅，张重权，王有琼，等.混菌固态发酵辣木粕的营养价值和功能特性 ［J］.食品与生物技术学报，2024，43（5）：26-36.XING J M，ZHANG Z Q，WNAG Y Q，et al.Exploring the nutritional value and functional characteristics of moringa seed meal by mixed bacteria solid-state fermentation ［J］.Journal of Food Science and Biotechnology，2024，43（5）：26-36.（in Chinese ）
［9］ YEŞIM A，FURKAN A，TUĞBA B，et al.Molecular characterization and multifunctional evaluation of lactic acid bacteria isolated from traditional sourdough ［J］.International Journal of Food Microbiology，2024，423，110845.
［10］ 中华人民共和国国家标准.GB/T 4789.35—2016，食品微生物学检验 乳酸菌检验 ［S］.北京：中国标准出版社，2016.
［11］ 中华人民共和国国家卫生和计划生育委员会.GB 4789.15—2016，食品安全国家标准 食品微生物学检验 霉菌和酵母计数 ［S］.北京：中国标准出版社，2017.
［12］ 迪娜热尔·迪力达西，刘璐，加勒哈斯别克·塞力克，等.新疆传统发酵乳品中乳酸菌与酵母菌的益生特性［J］.中国微生态学杂志，2018，30（1）：5-9.DILIDAXI D，LIU L，JIALEHASI BIEKE S，et al.Probiotic characteristics of the lactic acid bacteria and yeasts in Xinjiang traditional fermented dairy products ［J］.Chinese Journal of Microecology 2018，30（1）：5-9.（in Chinese）
［13］ 韩岳峰，焦明，马慧君，等.鼠李糖乳杆菌细菌素 WO-A/2的表达及其抗菌特性 ［J］.食品与生物技术学报，2024，43（05）：19-25.HAN Y F，JIAO M，MA H J，et al.Expression and antibacterial properties of bacteriocin WO-A/ 2 from Lactobacillus rhamnosus ［J］.Journal of Food Science and Biotechnology，2024，43（05）：19-25.（in Chinese ）
［14］ CHEVALLIER S，ZÚÑIGA R，LE-BAIL A.Assessment of bread dough expansion during fermentation［J］.Food & Bioprocess Technology，2012，5（2）：609-617.
［15］ 李岭卓，李锐，熊小毛，等.发酵液中乙醇含量的测定方法［J］.酿酒科技，2018（4）：126-128.LI L Z，LI R，XIONG X M，et al.Determination of ethanol content in fermenting liquid ［J］.Liquor-Making Science & Technology，2018（4）：126-128.（in Chinese ）
［16］ NUOBARIENE L，HANSEN A S，JESPERSEN L，et al.Phytase-active yeasts from grain-based food and beer ［J］.Journal of Applied Microbiology，2011，110（6）：1370 -1380.
［17］ XIAO Z Z，STORMS R，TSANG A.A quantitative starch-iodine method for measuring alpha-amylase and glucoamylase activities［J］.Analytical Biochemistry，2006，351（1）：146-148.
［18］ CUPP-ENYARD C.Sigma’s non-specific protease activity assay：casein as a substrate ［J］.Journal of Visualized Experiments，2008，19：899.
［19］ 方鲁平.优良乳酸菌和酵母菌发酵液体酸面团的品质和蛋白组分变化研究 ［D］.杭州：浙江工商大学，2022.
［20］ 周瑶，李娟，贾凤霞，等.单一及混合乳酸菌发酵对番木瓜汁品质的影响 ［J］.食品与机械，2024，40（1）：175-182.ZHOU Y，LI J，JIA F X，et al.Lactic acid fermentation of papaya juice using single and mixed lactic acid bacteria ［J］.Food & Machinery，2024，40（1）：175-182.（in Chinese ）
［21］ SU X Q，WU F F，ZHANG Y Q，et al.Effect of organic acids on bread quality improvement ［J］.Food Chemistry，2019，278：267-275.
［22］ MOHAMMADI-KOUCHESFAHANI M，HAMIDI-ESFAHANI Z，AZIZI M H.Isolation and identification of lactic acid bacteria with phytase activity from sourdough ［J］.Food Science & Nutrition，2019，7（11）：3700 -3708.
［23］ KEVSER K，OSMAN S，M.ZEKI D.Use of phytase active yeasts and lactic acid bacteria isolated from sourdough in the production of whole wheat bread ［J］.LWT-Food Science and Technology，2018，91：557-567.
［24］ AMAPU T Y，AMEH J B，ADO S A，et al.Amylolytic potential of lactic acid bacteria isolated from wet milled cereals，cassava flour and fruits ［J］.Microbiology Research Journal International，2016，13（2）：1-8.
［25］ 陈洁，李璞，王稳新，等.马铃薯生全粉对小麦粉面团特性的影响 ［J］.粮食与油脂，2018，31（7）：41-44.CHEN J，LI P，WANG W X，et al.Effect of potato raw powder on the characteristics of wheat flour dough ［J］.Cereals & Oils，2018，31（7）：41-44.
［26］ MCCANN T H，GALL M L，LI D.Extensional dough rheology：impact of flour composition and extension speed［J］.Journal of Cereal Science，2016，69：228-237.
［27］ SU T C，DU W K，DENG B Y，et al.Effects of sodium carboxymethyl cellulose on storage stability and qualities of different frozen dough ［J］.Heliyon，2023，9（8）：e18545.
［28］ SUN W，ZHANG W，XU Y，et al.Dough rheology，pasting property，and steamed bread quality of wheat flour as affected by the addition of sprouted wheat flour［J］.International Journal of Food Properties，2023，26（1）：2160 -2170.
［29］ BÁRBARA B，MARIANA M，NOOSHIN V，et al.Dough rheology and internal structure of bread produced with wheat flour partially substituted by buckwheat flour：a step towards enhancing nutritional value ［J］.Food Structure，2024，39，100364.
［30］ DOBRASZCZYK B J，SALMANOWICZ B P.Comparison of predictions of baking volume using large deformation rheological properties ［J］.Journal of Cereal Science，2008，47（2）：292-301.