Bifidobacterium adolescentis FHNFQ 5M4 Modulates Gut Microbiota and Alleviates Colitis in Mice

Authors

Yi WANG, Sinopharm Xingsha Pharmaceutical (Xiamen ) Co., Ltd ., Xiamen 361026 , China;Xiamen Key Laboratory of Maternal and Infant Health and Nutrition Products , Xiamen 361026 , China
Liming HUANG, School of Food Science and Technology , Jiangnan University , Wuxi 214122 , China
Xihua YU, Sinopharm Xingsha Pharmaceutical (Xiamen ) Co., Ltd ., Xiamen 361026 , China;Xiamen Key Laboratory of Maternal and Infant Health and Nutrition Products , Xiamen 361026 , China
Liuruolan LI, Sinopharm Xingsha Pharmaceutical (Xiamen ) Co., Ltd ., Xiamen 361026 , China;Xiamen Key Laboratory of Maternal and Infant Health and Nutrition Products , Xiamen 361026 , China
Wenwei LU, School of Food Science and Technology , Jiangnan University , Wuxi 214122 , China
Hongchao WANG, School of Food Science and Technology , Jiangnan University , Wuxi 214122 , China
Zhijian ZHANG, The Affiliated Wuxi Peopl e’s Hospital of Nanjing Medical University , Wuxi 214023 , China;Wuxi Medical Center of Nanjing Medical University , Wuxi 214023 , ChinaFollow

Corresponding Author(s)

张志坚（1981—），男，博士，主任医师，主要从事肾脏病预防与治疗研究。E-mail：zhangzhijian@njmu.edu.cn

Abstract

[Objective ] This study aims to verify and screen the Bifidobacterium adolescentis strains with vitamin A (VA) metabolic capacity,and investigate their effects on colitis symptoms and gut microbiota in mice.[Method ] The VA metabolic capacity of a panel of Bifidobacterium adolescentis strains was evaluated through in vitro fermentation,and the strain with the highest VA metabolic capacity was ultimately selected.This strain was administered orally to mice with dextran sulfate sodium (DSS )-induced colitis for 6 consecutive days.After the intervention,histopathological changes in the colonic tissue were assessed,and the levels of intestinal injury markers in the serum and cytokines,antioxidant enzymes,and retinoic acid (RA) in the colonic tissue were measured.Additionally,gut microbiota composition was analyzed by metagenomic sequencing.[Result ] Bifidobacterium adolescentis FHNFQ 5M4 which possessed the highest VA metabolic capacity sig nificantly alleviated the damage induced by DSS,modulated the cytokine and RA levels,and mitigated the oxidative stress in mouse colonic tissue.Moreover,Bifidobacterium adolescentis FHNFQ 5M4 markedly altered the gut microbiota composition in colitis mice,leading to enrichment of Butyricimonas and reduced relative abundance of Staphylococcus.[Conclusion ] Bifidobacterium adolescentis FHNFQ 5M4 can effectively modulate the gut microbiota and alleviate DSS-induced colitis symptoms in mice,demonstrating the potential as a probiotic candidate for the treatment of ulcerative colitis (UC).

Publication Date

8-15-2025

First Page

49

Last Page

60

DOI

10.12441/spyswjs.20250601001

Recommended Citation

WANG, Yi; HUANG, Liming; YU, Xihua; LI, Liuruolan; LU, Wenwei; WANG, Hongchao; and ZHANG, Zhijian (2025) "Bifidobacterium adolescentis FHNFQ 5M4 Modulates Gut Microbiota and Alleviates Colitis in Mice," Journal of Food Science and Biotechnology: Vol. 44: Iss. 8, Article 6.
DOI: 10.12441/spyswjs.20250601001
Available at: https://spsw.spyswjs.cnjournals.com/journal/vol44/iss8/6

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