Efficient Catalytic Synthesis of Allitol in Recombinant Escherichia coli

Authors

Ying CAO, School of Biotechnology , Jiangnan University , Wuxi 214122 , China;Key Laboratory of Industrial Biotechnology , Ministry of Education , Jiangnan University , Wuxi 214122 , China
Peiyu MIAO, School of Biotechnology , Jiangnan University , Wuxi 214122 , China
Yun LIU, School of Biotechnology , Jiangnan University , Wuxi 214122 , China
Zigang ZHANG, School of Biotechnology , Jiangnan University , Wuxi 214122 , China
Xian ZHANG, School of Biotechnology , Jiangnan University , Wuxi 214122 , China
Zhiming RAO, School of Biotechnology , Jiangnan University , Wuxi 214122 , China;Key Laboratory of Industrial Biotechnology , Ministry of Education , Jiangnan University , Wuxi 214122 , China;Institute of Future Food Technology , JITRI , Wuxi 214200 , ChinaFollow

Corresponding Author(s)

饶志明（1975—），男，博士，教授，博士研究生导师，主要从事微生物代谢工程与合成生物学研究。E-mail：rao-zhm@jiangnan.edu.cn

Abstract

[Objective ] This study aims to enhanced the conversion efficiency of allitol through biocatalytic methods.[Methods ] The ribulose dehydrogenase (RDH ) genes rdh from Providencia alcalifaciens,Klebsiella pneumoniae and Klebsiella oxytoca were respectively expressed in Escherichia coli (E.coli ) BL21,designated as Pardh,Kprdh and Kordh respectively to identify the target genes with higher transformation rates.Further,the RDH from Klebsiella oxytoca was coupled with the formate dehydrogenase (FDH ) from Candida boidinii for co-catalysis,and the catalytic conditions and the addition amounts of co-substrates were optimized to achieve efficient enzymatic catalytic synthesis of allitol.[Results ] When using FDH and RDH from Klebsiella pneumoniae with an initial substrate D-piscose of 50 g/L and continuous feeding over 6 h to a total concentration of 100 g/L,in the presence of sodium formate as the auxiliary substrate,the conversion rate of allitol reached 53.3%.[Conclusion ] By coupling the RDH and FDH systems,the conversion rate of allitol can be increased.

Publication Date

8-15-2025

First Page

22

Last Page

30

DOI

10.12441/spyswjs.20240301002

Recommended Citation

CAO, Ying; MIAO, Peiyu; LIU, Yun; ZHANG, Zigang; ZHANG, Xian; and RAO, Zhiming (2025) "Efficient Catalytic Synthesis of Allitol in Recombinant Escherichia coli," Journal of Food Science and Biotechnology: Vol. 44: Iss. 8, Article 3.
DOI: 10.12441/spyswjs.20240301002
Available at: https://spsw.spyswjs.cnjournals.com/journal/vol44/iss8/3

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