Co-expression and Application of Enzymes for Preparation of β-Nicotinamide Mononucleotide

Authors

Jiehu LIU, School of Biotechnology , Jiangnan University , Wuxi 214122 , China;Key Laboratory of Industrial Biotechnology , Ministry of Education , Jiangnan University , Wuxi 214122 , China;State Key Laboratory of Food Science and Resources , Jiangnan University , Wuxi 214122 , China
Lingqia SU, School of Biotechnology , Jiangnan University , Wuxi 214122 , China;Key Laboratory of Industrial Biotechnology , Ministry of Education , Jiangnan University , Wuxi 214122 , China;State Key Laboratory of Food Science and Resources , Jiangnan University , Wuxi 214122 , China
Huixian FU, School of Biotechnology , Jiangnan University , Wuxi 214122 , China;Key Laboratory of Industrial Biotechnology , Ministry of Education , Jiangnan University , Wuxi 214122 , China;State Key Laboratory of Food Science and Resources , Jiangnan University , Wuxi 214122 , China
Jing WU, School of Biotechnology , Jiangnan University , Wuxi 214122 , China;Key Laboratory of Industrial Biotechnology , Ministry of Education , Jiangnan University , Wuxi 214122 , China;State Key Laboratory of Food Science and Resources , Jiangnan University , Wuxi 214122 , ChinaFollow

Corresponding Author(s)

吴敬（1969—），女，博士，教授，博士研究生导师，主要从事酶工程和发酵工程研究。Email：jingwu@jiangnan.edu.cn

Abstract

[Objective ] This study aims to reduce the cost and simplify the production process of β-nicotinamide mononucleotide (β-NMN ) by the enzyme catalytic method.[Method ] On the basis of the previously established β-NMN synthesis system with six enzymes,the six enzyme genes were combined in pairs to construct double-enzyme co-expression recombinant strains.Each crude enzyme solution obtained by co-expression was used for multi-enzyme catalytic cascade reaction to synthesize β-NMN.[Result ] The yield of β-NMN was as high as 72.90% when the concentration of the substrate D-ribose was 10 mmol/L.The optimal temperature and IPTG induction concentration for inducing the enzyme expression of the recombinant strains were 25 ℃ and 0 mmol/L,respectively.Finally,the sa lt ions and polyhydroxy compounds that significantly improved the storage stability of 5-phosphate ribose- 1-pyrophosphate synthase (EcPrs) were identified.Mn2+,lactose,and sorbitol basically had no effect on the yield of β-NMN.[Conclusion ] The multi-enzyme catalytic cascade reaction for the preparation of β-NMN under dual-enzyme co-expression system and the optimization for EcPrs storage stability provide a new idea for the enzyme catalytic synthesis of β-NMN.

Publication Date

8-15-2025

First Page

139

Last Page

147

DOI

10.12441/spyswjs.20240103001

Recommended Citation

LIU, Jiehu; SU, Lingqia; FU, Huixian; and WU, Jing (2025) "Co-expression and Application of Enzymes for Preparation of β-Nicotinamide Mononucleotide," Journal of Food Science and Biotechnology: Vol. 44: Iss. 8, Article 15.
DOI: 10.12441/spyswjs.20240103001
Available at: https://spsw.spyswjs.cnjournals.com/journal/vol44/iss8/15

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