Extraction Process Optimization and Antioxidant and Blood Glucose-lowering Effect Evaluation of Polyphenols from Camellia japonica Flowers

Authors

Long CHEN, School of Food Science and Engineering , Xinyang Agriculture and Forestry University , Xinyang 464399 , China ;Henan Engineering Technology Research Center for Comprehensive Utilization of Characteristic Food Resources in Dabie Mountains , Xinyang 464399 , China
Xiaocong YANG, School of Food Science and Engineering , Xinyang Agriculture and Forestry University , Xinyang 464399 , China ;Henan Engineering Technology Research Center for Comprehensive Utilization of Characteristic Food Resources in Dabie Mountains , Xinyang 464399 , China
Shujie XING, Henan Engineering Technology Research Center for Comprehensive Utilization of Characteristic Food Resources in Dabie Mountains , Xinyang 464399 , China ;Institute of Food Technology and Innovation , Luohe Vocational Technology College , Luohe 462002 , China
Xinyao SHI, School of Food Science and Engineering , Xinyang Agriculture and Forestry University , Xinyang 464399 , China
Xueke ZHENG, School of Food Science and Engineering , Xinyang Agriculture and Forestry University , Xinyang 464399 , China ;Henan Engineering Technology Research Center for Comprehensive Utilization of Characteristic Food Resources in Dabie Mountains , Xinyang 464399 , China
Jing ZHU, School of Food Science and Engineering , Xinyang Agriculture and Forestry University , Xinyang 464399 , China ;Henan Engineering Technology Research Center for Comprehensive Utilization of Characteristic Food Resources in Dabie Mountains , Xinyang 464399 , ChinaFollow

Corresponding Author(s)

朱静（1982—），女，博士，教授，硕士研究生导师，主要从事发酵食品研究。E-mail：zhujingcy@163.com

Abstract

[Objective ] This study optimized the extraction process of polyphenols from Camellia japonica (C.japonica ) flowers and preliminarily explored the antioxidant and blood glucose-lowering effects of the extracted polyphenols,aiming to improve the utilization of C.japonica flowers.[Method ] With the flowers of Xinyang Maojian as raw materials,the ultrasonic-assisted method was adopted to extract polyphenols from C.japonica flowers,and the antioxidant and blood glucose-lowering activities of the extracted polyphenols were analyzed.With the extraction rate of polyphenols from C.japonica flowers as the evaluation index,the response surface methodology was adopted to explore the effects of ethanol volume fraction,solid-liquid ratio,extraction time,and ultrasonic power on the extraction rate of polyphenols from C.japonica flowers.[Result ] Under the conditions of the ethanol volume fraction of 54%,the solid-liquid ratio of 1 g∶33 mL,the extraction time of 49.5 min,and the ultrasonic power of 210 W,the actual extraction rate obtained from verification experiment was 12.60%,with a relative standard deviation (RSD ) of 2.28%,which indicated that the optimization results were reliable.In addition,the polyphenols from C.japonica flowers had strong scavenging abilities against DPPH,hydroxyl,and ABTS+ free radicals.The scavenging abilities were positive correlated with the mass concentration of the extracted polyphenols,and the half-inhibitory mass concentrations (IC 50) were 0.044,0.065,and 0.068 mg/mL,respectively.The extracted polyphenols had good inhibitory effects on the activities of α-glucosidase and α-amylase,with the IC 50 of 0.054 and 0.057 mg/mL,respectively.[Conclusion ] The polyphenols from C.japonica flowers can be used as a good natural antioxidant and blood glucose-lowering agent,demonstrating potential application value.This study provides a theoretical foundation for the refined processing of C.japonica flowers.

Publication Date

8-15-2025

First Page

97

Last Page

106

DOI

10.12441/spyswjs.20241207001

Recommended Citation

CHEN, Long; YANG, Xiaocong; XING, Shujie; SHI, Xinyao; ZHENG, Xueke; and ZHU, Jing (2025) "Extraction Process Optimization and Antioxidant and Blood Glucose-lowering Effect Evaluation of Polyphenols from Camellia japonica Flowers," Journal of Food Science and Biotechnology: Vol. 44: Iss. 8, Article 11.
DOI: 10.12441/spyswjs.20241207001
Available at: https://spsw.spyswjs.cnjournals.com/journal/vol44/iss8/11

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