Effects of Ultrasound-assisted Enzymatic Extraction on the Physical and Chemical Properties of Tortoise Shell Collagen

Authors

Xiong HE, School of Food and Biological Engineering , Hefei University of Technology , Hefei 230601 , China
Rongxing LI, School of Food and Biological Engineering , Hefei University of Technology , Hefei 230601 , China
Shaotong JIANG, School of Food and Biological Engineering , Hefei University of Technology , Hefei 230601 , China;Anhui Province Key Laboratory of Agricultural Products Modern Processing , Hefei 230601 , China
Jianfeng LU, School of Food and Biological Engineering , Hefei University of Technology , Hefei 230601 , China;Anhui Province Key Laboratory of Agricultural Products Modern Processing , Hefei 230601 , China;Engineering Research Center of Bio-process , Ministry of Education , Hefei 230601 , China
Lin LIN, School of Food and Biological Engineering , Hefei University of Technology , Hefei 230601 , China;Anhui Province Key Laboratory of Agricultural Products Modern Processing , Hefei 230601 , China;Engineering Research Center of Bio-process , Ministry of Education , Hefei 230601 , ChinaFollow

Corresponding Author(s)

林琳（1978—），女，博士，副教授，硕士研究生导师，主要从事水产品贮藏及加工、水产品加工副产物综合利用研究。E-mail：linlin@hfut.edu.cn

Abstract

[Objective ] The effects of different extraction methods on the yield,purity,structural characteristics,and physicochemical properties of tortoise shell collagen were investigated.[Method ] The ultrasound-assisted enzymatic method,acid method,and enzymatic method were used to extract collagen from tortoise shell powder.[Result ] The yield and purity of tortoise shell collagen extracted by ultrasound-assisted enzymatic method were 2.29% and 85.65%,respectively,significantly higher than those extracted by acid method (0.78% and 73.15%) and enzymatic method (1.77% and 75.63%).Ultraviolet absorption spectroscopy,infrared absorption spectroscopy,circular dichroism spectroscopy,and electrophoresis profiles showed that collagens extracted from tortoise shell by three methods were all type I collagen and retained intact triple helix structures.X-ray diffraction patterns revealed that the molecular structure of tortoise shell collagen treated with ultrasound-assisted enzymatic method was more compact,with a higher degree of order.The particle sizes of tortoise shell collagens extracted by ultrasound-assisted enzymatic method and enzymatic method were both distributed around 340 nm,while tortoise shell collagen extracted by ultrasound-assisted enzymatic method exhibited a lower particle size distribution.The thermal denaturation temperatures (Td) of the tortoise shell collagens extracted by the three methods were all 44 ℃.Scanning electron microscopy results showed that the microstructure of tortoise shell collagen extracted by ultrasound-assisted enzymatic method was more uniform and regular.[Conclusion ] The combination of ultrasound treatment and enzymatic methods provide a new perspective for the extraction of collagen from tortoise shell.Compared with the conventional acid method and enzymatic method,the ultrasound-assisted enzymatic extraction method shows improvements in the yield and purity of tortoise shell collagen,thus demonstrating a broad application prospect.

Publication Date

10-15-2025

First Page

152

Last Page

162

DOI

10.12441/spyswjs.20240911001

Recommended Citation

HE, Xiong; LI, Rongxing; JIANG, Shaotong; LU, Jianfeng; and LIN, Lin (2025) "Effects of Ultrasound-assisted Enzymatic Extraction on the Physical and Chemical Properties of Tortoise Shell Collagen," Journal of Food Science and Biotechnology: Vol. 44: Iss. 10, Article 16.
DOI: 10.12441/spyswjs.20240911001
Available at: https://spsw.spyswjs.cnjournals.com/journal/vol44/iss10/16

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