Recombinant Pichia pastoris Fermentation for Biosynthesis of the UV Resistant Compound Gadusol

Authors

Wenjie ZUO, Science Center for Future Foods , Jiangnan University , Wuxi 214122 , China;School of Biotechnology , Jiangnan University , Wuxi 214122 , China;Jiaxing Institute of Future Food , Jiaxing 314001 , China
Ping ZHANG, Science Center for Future Foods , Jiangnan University , Wuxi 214122 , China;School of Biotechnology , Jiangnan University , Wuxi 214122 , China
Ruirui XU, Science Center for Future Foods , Jiangnan University , Wuxi 214122 , China;School of Biotechnology , Jiangnan University , Wuxi 214122 , China
Leyi ZHANG, Science Center for Future Foods , Jiangnan University , Wuxi 214122 , China;School of Biotechnology , Jiangnan University , Wuxi 214122 , China
Zhen KANG, Science Center for Future Foods , Jiangnan University , Wuxi 214122 , China;School of Biotechnology , Jiangnan University , Wuxi 214122 , China
Jianghua LI, Science Center for Future Foods , Jiangnan University , Wuxi 214122 , China;School of Biotechnology , Jiangnan University , Wuxi 214122 , China;Jiaxing Institute of Future Food , Jiaxing 314001 , ChinaFollow

Corresponding Author(s)

李江华（1966—），男，博士，教授，博士研究生导师，主要从事发酵工程和酶工程等领域研究。E-mail：lijianghua@jiangnan.edu.cn

Abstract

[Objective ] This study aims to employ synthetic biology and metabolic engineering techniques to engineer Pichia pastoris (P.pastoris ) for the efficient heterologous synthesis of the UV resistant compound gadusol.[Method ] On the basis of a bioinformatic analysis and codon optimization of the genes involved in the gadusol biosynthetic pathway in Danio rerio,the gadusol fermentation pathway was constructed in P.pastoris via strategies such as promoter engineering,metabolic engineering,and fermentation optimization.[Result ] A high-purity gadusol standard was prepared through optimized extraction and chromatographic separation.The gadusol yield in the recombinant strain P.pastoris HME reached 105 mg/L.By screening of gene expression promoters and optimization of the fermentation medium components,the engineered strain,P.pastoris GME,achieved a gadusol yield of approximately 175 mg/L.[Conclusion ] This study successfully constructed the strain P.pastoris GME capable of efficiently synthesizing gadusol,providing support and a reference for accelerating the industrial production of gadusol by fermentation and the research on the application of this compound in the food and cosmetic industries.

Publication Date

10-15-2025

First Page

103

Last Page

112

DOI

10.12441/spyswjs.20240321002

Recommended Citation

ZUO, Wenjie; ZHANG, Ping; XU, Ruirui; ZHANG, Leyi; KANG, Zhen; and LI, Jianghua (2025) "Recombinant Pichia pastoris Fermentation for Biosynthesis of the UV Resistant Compound Gadusol," Journal of Food Science and Biotechnology: Vol. 44: Iss. 10, Article 11.
DOI: 10.12441/spyswjs.20240321002
Available at: https://spsw.spyswjs.cnjournals.com/journal/vol44/iss10/11

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